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Dr_fungus (Dr_fungus)
Senior Member
Username: Dr_fungus

Post Number: 102
Registered: 01-2004
Posted on Sunday, March 07, 2004 - 04:56 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Morning fellow topians,
I have a glovebox made of cardboard and wanted to make a few syringes for microscopy use. I have previously closed her up and sprayed the inside with lysol, let set for 1 hr and began work. I also wipe down the gloves and shotglass with 70% alcohol, before i begin work. I was wondering if I really need to spray the inside or would it be fine without, since there's no airflow inside the box? Any help would be appreciated
"Freedom is not something that anybody can be given; freedom is something people take and people are as free as they want to be."
-James Baldwin
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 15061
Registered: 02-2001
Posted on Sunday, March 07, 2004 - 05:05 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

as long as it's stayed sealed
you haven't added anything
it should still be good
of course it never hurts to be careful
but too much disinfectant inside can
be problematic.
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Taoist Shredder (Taoistshredder)
Senior Member
Username: Taoistshredder

Post Number: 186
Registered: 01-2004
Posted on Sunday, March 07, 2004 - 05:06 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I use a tupperware glove box (c/o Triple Helix), and I Lysol it, close it up, turn the fan on, and go to work 15 minutes later. It gets to the point where, if you let a previously "Lysol-sterilized" area chill for an hour, the Lysol loses its effectiveness. Realistically, it loses it's effectiveness as soon as it starts to dry. However, if there really is NO airflow whatsoever in your box (my box has a fan inside that takes external air through a filter and pushes excess air out of the boxes seams), it should remain sterile for a long time after you spray it. If it's cardboard, I've got to believe it breathes, though. I also use 91% alcohol, but I don't think it matters a whole lot.
In Tyler we trust.
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 15063
Registered: 02-2001
Posted on Sunday, March 07, 2004 - 05:12 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

be sure not to light a spark or flame
in a sealed box filled with lysol fumes...

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jimjim (Jimjim)
Senior Member
Username: Jimjim

Post Number: 151
Registered: 06-2003
Posted on Sunday, March 07, 2004 - 05:12 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I previously used a cardboard glovebox that I lined 100% w/foil (spray adhesive and elec.tape). I continually bombarded it with bleach/H2O, so as if to err, it was on the side of caution. I had(have) a VERY disadvantageous environment with regards to general sterility (carpets, indoor pets, dirty laundry ), so I try to eliminate/control any potential sources of contamination whenever I can........jimjim
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Dr_fungus (Dr_fungus)
Senior Member
Username: Dr_fungus

Post Number: 103
Registered: 01-2004
Posted on Sunday, March 07, 2004 - 05:14 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Well Realistically cardboard does breath, but what i mean is that it should'nt let contams in once I closed it right, the cardboard would be more like a filter wouldn't it? I was also just wondering if the lysol would settle out in an hour, I wouldn't want it airborn when I open my print and begin work.
"Freedom is not something that anybody can be given; freedom is something people take and people are as free as they want to be."
-James Baldwin
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 15066
Registered: 02-2001
Posted on Sunday, March 07, 2004 - 05:18 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

an hour is adequate.
Oust is better than lysol.
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Dr_fungus (Dr_fungus)
Senior Member
Username: Dr_fungus

Post Number: 104
Registered: 01-2004
Posted on Sunday, March 07, 2004 - 05:21 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Thank's for the advice
"Freedom is not something that anybody can be given; freedom is something people take and people are as free as they want to be."
-James Baldwin
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NegativeZer0 (Negativezer0)
Senior Member
Username: Negativezer0

Post Number: 215
Registered: 12-2003
Posted on Sunday, March 07, 2004 - 10:00 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

ive always heard dilute bleach; wont warp the plstic of your using plastic; wont catch on fire like lysol....?
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 15081
Registered: 02-2001
Posted on Sunday, March 07, 2004 - 10:04 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

correct.
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NegativeZer0 (Negativezer0)
Senior Member
Username: Negativezer0

Post Number: 222
Registered: 12-2003
Posted on Monday, March 08, 2004 - 12:50 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

what ratio?
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faht (Fahtphish)
Senior Member
Username: Fahtphish

Post Number: 913
Registered: 01-2003
Posted on Monday, March 08, 2004 - 12:59 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

probably about the same as you would use on your kitchen counter tops... maybe a little more for good measure. too much can be harmful for your skin tho. maybe a 100/1 water bleach ratio. double what you would use for bleach dips. :-)

fahtster
says: hello :-)
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NegativeZer0 (Negativezer0)
Senior Member
Username: Negativezer0

Post Number: 223
Registered: 12-2003
Posted on Monday, March 08, 2004 - 01:10 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

thanks faht; i think sometime there should be a fahts cake tips along with scotsmans...do you clone much?
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faht (Fahtphish)
Senior Member
Username: Fahtphish

Post Number: 915
Registered: 01-2003
Posted on Monday, March 08, 2004 - 01:18 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

nah, haven't tried it yet. lol i don't need no stinking section. hehe im always here. besides scotsman got it pretty much covered. but thanx for the thought. i really do take it to heart. :-)

much love
fahtty

says: hello :-)
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Realschmeal (Mindovermycelia)
Member
Username: Mindovermycelia

Post Number: 24
Registered: 02-2004
Posted on Monday, March 08, 2004 - 03:05 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I posted about this in another thread, but I will bring it up here: If you put an ozonator near the intake of a glove box like Triple sells wouldn't that do as much to sterilize as lysol or do the hepa filters somehow stop the ozone from entering?I know they are a bit expensive, but they are great for the home as well as the hobby. I love my Eco-Quest.
I'm feeling trapped in my own body - Alice Donut
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NegativeZer0 (Negativezer0)
Senior Member
Username: Negativezer0

Post Number: 224
Registered: 12-2003
Posted on Monday, March 08, 2004 - 05:05 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

when i saw scotsmans' cakes i almost cried; and when i saw your FOAF's i did so far faht; i think your FOAF is the best at cakes; you should be proud of him!
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Realschmeal (Mindovermycelia)
Member
Username: Mindovermycelia

Post Number: 28
Registered: 02-2004
Posted on Monday, March 08, 2004 - 03:50 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I did more research here:
http://www.epa.gov/iaq/pubs/ozonegen.html#how%20is %20ozone%20harmful
and found that what I have read in some posts here about ozone being a good air sanitizer for mycological purposes may not be true not to mention the manufacturer's claims. Other plants are known to be adversely affected by ozone. Sanitizing a glove box like this with a ventilation unit would be easy without ozone. I answer my own question: An ozonator probably would sanatize as well for glove boxes however I would refrain from using near the intake of a terrarium humidifier for sterilization purposes.
I'm feeling trapped in my own body - Alice Donut
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 15189
Registered: 02-2001
Posted on Tuesday, March 09, 2004 - 02:28 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

good link/reading there,
thx
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 15190
Registered: 02-2001
Posted on Tuesday, March 09, 2004 - 02:29 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

archive material