Post Number: 19546
|Posted on Friday, May 21, 2004 - 02:26 am:||
I plan on letting the 1/2 pint cakes fully colonize to make sure there are no contams. Then I will make a Myc syringe out of them and innoculate the quart jars from that.
I've been doing a REALLY easy tek with making myc. syringes and isolating a substrain at the same time. First, you take a 1/2 pint jar and fill just the bottom of the jar with a grain (i use popcorn), so like a 1/2 inch of grain.. not much at all. then cover with two layers of tyvek and PC like normal. then when it's done and cool i poke a hole in the center of the tyvek and put two drops of spore solution in middle of the grain. Now you DON'T want to disturb this at all.. NO shaking, nothing... you want the spores to germinate as close together at you can so that one substrain (the dominate one) takes over the rest. Now i let this colonize the small amount of grain in the bottom of the 1/2... you can even let it go as far as pinning or small mushies to see what kind of fruiting strain you have, it doesn't matter, as it's not going to be exposed to the outside air at all. Now once your substrain has colonized the grain to your satisfaction, you can go ahead and break up the grain by shaking.. this will break off bits of myc. Now poke thru the tyvek with your syringe of sterilized plain water, shoot it in the jar, swish it around and suck the water back up. you should now have a syringe full of mycelium laden solution.. use this to innoc your qt jars. This is basically a g2g without opening any jars or any use of gloveboxes or flowhoods and minus the agar. the best part about this is you can also use fully colonized grain jars to get your innoc from THEN you can lay out that jar and fruit it!! so you don't even lose any spawn grain.
Post Number: 1134
Posted on Thursday, May 20, 2004 - 10:55 pm:
one more thing.. if you plan on using qt colonized jars to get your innoc from... plan on losing some water solution in the grain bout a quarter of the origional syringe solution... thats why i use 1/2 pints... less surface area to lose water to.