Making a spore syringe Return To Archives | Search

Please Visit Our New Forums at Mycotopia
Please visit our Sponsors

Mycotopia Web Archive Archive Spores, Prints & Syringes Making a spore syringe Previous Next

ClosedClosed: New threads not accepted on this page
Topic Author Last Poster Posts Pages Last Post

Top of pageBottom of pageLink to this message

Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 21083
Registered: 02-2001
Posted on Friday, June 11, 2004 - 01:45 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

from our sponsor
http://www.mushmush.nl

To make a spore syringe you will need the following items:

Inoculation loop/scalpel
(something to pick up the spores)

Spore print

Syringe and needle (sterile)

Small amount of sterilized water in a bottle (capped with tinfoil)

Clean flame source (gas- or alcohol burner)

Latex gloves (optional)

Upload

The most important thing in the whole process is to work clean. If possible perform the following procedure in a glovebox or HEPA filtered environment. If you do not have access to one of these you can perform it in the open air, but chances of success are smaller. If available wear gloves that have been disinfected with alcohol 70% or Lysol.
CAUTION: Most disinfectants are flammable, use extreme caution when using these in combination with an open flame!.

Before you start clean the working surface with alcohol or Lysol. Allow it to dry before proceeding!

Upload

The inoculation loop or scalpel is flamed until red hot, and left to cool. Alternatively you can use a pre-sterilized inoculation loop.

Upload

Peal back the tinfoil of the flask so that it can be removed easily but leave it covering the flask. Open the baggie with the spore print and scrape some of the spores of the paper. If the loop is too hot the spores will be killed or the plastic will melt to it.:

Upload

Deposit the spores into the water and quickly replace the tinfoil. Repeat if not enough spores have been transferred. If the spores are visible in the water there should be enough.

Upload

The syringe is assembled. Most syringes can be reused by sterilizing them in a pressure cooker. Just wrap them in tinfoil and sterilize for 25-30 minutes.

Upload

Some spore suspension is sucked up in the syringe. Pull the plunger back and forth a few times to disperse the spores in the water. Syringes can then be filled with spore suspension.

Upload

The needle cap is replaced and the syringes are put in the fridge until ready to use. Don't forget to label them with strain and date. Syringes can be kept like this for several months after which they become noticeably less viable.

Upload

archive material

www.mycrotopia.com