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rope-like (Lucid1)
Member
Username: Lucid1

Post Number: 24
Registered: 06-2004
Posted on Saturday, June 26, 2004 - 06:40 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I read in a post at one time about a tek involving a needle and a fruit body. The poster called it coring. I looked and didnt find a writeup on it. Millie used my interpretation of the technique though and it worked wonderfully.

Finding a fruit body that was growing well, Millie plucked it.

Premade karo jars, 1/2 pint, with a small dollop of karo, and polyfill, PCd for 30 mins at 15lbs on medium heat were gathered to the semi sterile area.

I say semi-sterile, if there is a glovebox available there is no need for this technique.

Using a sterilized syringe and needle, and sterilizing all other instruments with isopropyl alcohol, she began. She first cut into the stem of the fruit, and again about 1cm up from the first cut. After that, she drew into the syringe a small amount of sterile H2O, and a bit of air from the sterile H2O jar. -This last is important so the needle is empty for the coring.

After this she ran the sample through with the needle, making sure that fleh from the fruit was sliding into the needle visually. In one swift movement, only pausing for an alcohol swab across the needle, she penetrated the polyfill on the karo jar and deposited the flesh to float.

It has been a substancial amount of time now and all seems very well, the karo jars are so clear you can see very well anything growing in them. There is only a small amount of fuzz coming directly from the flesh.

Again this tek has probably already been written up elsewhere, but I wanted to make sure it was remembered. Many people like the even flushes that come with a clone from a favored fruit, but for whatever reason dont have a glovebox and are scared by agar. This seems to be a great tek for them.



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Imok Urok2 (Imok)
Moderator
Username: Imok

Post Number: 1590
Registered: 07-2002
Posted on Saturday, June 26, 2004 - 12:13 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

The only problem i can see with this adaptation of the
cloning tek is that you didn't macerate (cut up into small bits)
the sample of flesh taken from the inside of the shroom.
But since the sample is so small anyway it shouldn't
be a major problem in it being successful that I can see.

Otherwise, it sounds like a passable method to use when,
like you said, you don't have a flowhood/glove box and
are working in a semi-sterile area.

I don't recall hearing about this method before myself.
In searching this site I only came up with one thread
that wasn't about "coring cakes" and that was only an
allusion to a "coring tek" used in cloning.
http://archives.mycotopia.net/discus/messages/2/146843. html#POST223092
Maybe Hip knows where the original is.

Thanks for posting your version here anyway.
If we don't have it in the archives here already your
version might just make it there.
Hope this helps :-)
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rope-like (Lucid1)
Member
Username: Lucid1

Post Number: 34
Registered: 06-2004
Posted on Sunday, June 27, 2004 - 07:07 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Update on the coring tek, sufficient time has passed that the fruit chunks have started to sprout, well really like fuzz, but if you look real close it looks like roots. Either way, neither of the jars done with this tech have had contaminants!






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rope-like (Lucid1)
Member
Username: Lucid1

Post Number: 35
Registered: 06-2004
Posted on Sunday, June 27, 2004 - 07:11 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


quote:

I don't recall hearing about this method before myself.
In searching this site I only came up with one thread
that wasn't about "coring cakes" and that was only an
allusion to a "coring tek" used in cloning.
http://archives.mycotopia.net/discus/messages/2/146843. html#POST223092
Maybe Hip knows where the original is.




Thats funny, that post mentioning the tech was mine. Funny to me because I have only made a handfull of post.

If there is an original, I would like to be pointed to it, if there is not then... well use the tek in good health!
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Imok Urok2 (Imok)
Moderator
Username: Imok

Post Number: 1595
Registered: 07-2002
Posted on Monday, June 28, 2004 - 04:32 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Well, then I will say it and Hip can check it...
archive material
Hope this helps :-)
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rope-like (Lucid1)
Member
Username: Lucid1

Post Number: 50
Registered: 06-2004
Posted on Monday, June 28, 2004 - 07:23 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

well, I dont know entirely what that means, but this tech does IMO belong in the cloning section of the arkhives, as I couldnt find anything exactly like it there. Of course many of them there involve liquid cultures, and some involve syringes, but I havent found any that use this technique.

Again, I am not claming it as my own, but I think it is very usefull! It is the furute of cloning for me, very fast, and very easy! from the 4 Millie has done so far, 100% success.

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Imok Urok2 (Imok)
Moderator
Username: Imok

Post Number: 1596
Registered: 07-2002
Posted on Tuesday, June 29, 2004 - 01:03 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Moderators use that term to indicate good material, so
when the threads are sifted, in preparing to prune off
the dross, the valuable nuggets of information don't get
lost and thrown out with the bath water (to mix my metaphors).
In other words, the information should go into the archives
if it doesn't already exist there or if it can bring
a new perspective that our members may find of value.
As long as you are up front about it, it doesn't matter
if you didn't create it.
What is important is that you cared enough to share it.
Thanks for helping
Hope this helps :-)