  
drunker then u (Drunk)
| Posted on Wednesday, October 30, 2002 - 06:16 am: |
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this is a glovebox that i made it was cheap and easy i jus wanted to share here it is cheers all i had was a big fish tank and garbage bags i cleaned the fish tank very well then sat it on its side covered the top of it tightly with a garbage bag then i put 2 tiny holes that my hands would soon force open then i got a garbage bag that once i cut a small hole in it would go from my rist to my elbow with a little bit extra left and the same for another i put on gloves and forced my hands throw each of the holes and taped the gloves to the bags then force my hands throw the little holes i cut earlyer in the top of the fish tank it worked great cheers to beers |
  
mf (Fishbone)
| Posted on Wednesday, October 30, 2002 - 07:52 am: |
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that sounds good and when it's not in use the aquarium is just an aquarium |
  
drunker then u (Drunk)
| Posted on Wednesday, October 30, 2002 - 08:11 am: |
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no i clean it a tape atother garbage bag over the top but i guess u can use as a fish tank but i dont think your fish would be very happy with u wrecking there lives for your own satisfaction but thats just my opiniyon i just cant spell but i guess u only need it for 10 min to about a hour maby if u had a big bowl it wouldint matter but if u dont why not just buy another fish tank for that purpus well i guess u have to change there watter all the time thats a pretty good thing to bring up i guess when u need to change there water why not clean it well have clones and clean tank thats awsome never thought of it that way cheers
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Hippie3 (Admin)
| Posted on Wednesday, October 30, 2002 - 03:57 pm: |
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you just just get one of those aquarium back-drops with the fish and plants and stuff on it
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drunker then u (Drunk)
| Posted on Wednesday, October 30, 2002 - 08:18 pm: |
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great idea cheers |
  
fungusmaximus (Fungaloid)
| Posted on Thursday, October 31, 2002 - 12:27 am: |
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No fans, or air intake/outlet? |
  
drunker then u (Drunk)
| Posted on Thursday, October 31, 2002 - 05:58 am: |
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no its just to clone it worked for me if some one else where to use it and done clones befor im sure it would work for them since this was my first time my understanding was for clones u dont need that cheers |
  
Oldtimer (Oldtimer)
| Posted on Thursday, October 31, 2002 - 01:27 pm: |
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BUILDING A STERILE TRANSFER CHAMBER In order to achieve a good success rate when making transfers, you must first construct a transfer chamber or “glove box.” This is simply an enclosed area that can be cleaned with disinfectants to keep out contaminants. Such a chamber can be designed in many ways and need not be elaborate to work well. Basically what you are striving for is an enclosure which provides a sterile, draft-free environment to work in while performing sterile culture work. A good, inexpensive glove box can be constructed from a cardboard box, some aluminum foil and a sheet of plexiglass. You will need the following materials: Clean, sturdy cardboard box. Razor knife or box cutter Can of spray adhesive. Roll of heavy duty aluminum foil. Roll of duct tape. A piece of plexiglass cut to approximately 10” x 14”. Clean dish towel.
Step I Obtain a good, clean, sturdy cardboard box with a removable lid. The kind paper is shipped in is ideal and can be found at any printing shop or office supply company. Use a small dish or bowl as a template and draw two circles about 5” in diameter on the front of the box. Cut out the two circular holes in the front side of the box (to allow you to insert your arms while making transfers). Also cut a square hole in the lid of the box slightly smaller than the sheet of plexiglass (approx. 9” x 13”). This hole will be covered with the sheet of plexiglass, effectively forming a “window” in the top of the box. A razor knife or box cutter makes an ideal tool for cutting these holes in the box. Once you have the holes cut you can proceed to step II and prepare to apply the foil.
Step II Wipe the inside of the box with alcohol and allow it to dry. After the alcohol dries liberally spray the entire inner surface of the box (sides, bottom and top) with the supplied Lysol aerosol. This kills any spores that are lying dormant on the surface of the cardboard. Once the Lysol has evaporated, spray a layer of adhesive in the bottom of the box and apply a sheet of aluminum foil to cover. Do not worry if the foil has a few wrinkles or creases. You just want to have a nonporous surface that is easy to clean and sterilize as well as being waterproof. Repeat this process on all sides of the inside of the box.
Step III Fit the lid onto the box. Place the sheet of plexiglass over the hole in the lid of the box and tape each side to the box with duct tape. Be sure to seal each edge as you tape the plexiglass down to the box. This forms a nice, airtight “window” in the top of the box which will allow you see into the cabinet with no risk of contamination from airborne spores and bacteria. Leave the lid of the box removable (do not tape) to allow easy access for placing your materials inside prior to performing sterile transfer work.
STEP IV Take a small, clean dishtowel and drape it across the top of the box so that it hangs down over the two hand holes in the front. Run a strip of duct tape along the edge of the towel taping it to the lid of the box along the edge of the plexiglass. The towel serves as a barrier to prevent drafts from entering the cabinet while you are working, effectively keeping contaminants out. Do not tape the lid down. Leave the lid removable so it will be easier later when you need to load materials into and out of the sterile cabinet.
Congratulations! You now have a completed, fully functional sterile transfer cabinet that only cost a few bucks.... Spray inside of chamber with Lysol before each use but....ALLOW ALCOHOL FROM LYSOL to EVAPORATE COMPLETELY!!! before lighting any burners or lighters inside of box... unless you like the "hairless" look and don't mind your arms stinging like hell for a day or two! OT |
  
Hippie3 (Admin)
| Posted on Thursday, October 31, 2002 - 02:26 pm: |
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great post, as always, oldtimer. definite archive material there.
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drunker then u (Drunk)
| Posted on Thursday, October 31, 2002 - 03:57 pm: |
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tats really good lookin box cheers |
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