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Topic Author Last Poster Posts Pages Last Post
Loosid Gets FMRC's Ion Generatorloosidloosid16 1 09-12-03  03:31 am
Archive through November 07, 2004ohmatic (Ohmatic)ohmatic (Ohmatic)25 1 11-07-04  08:16 pm
Archive through November 09, 2004Rio (Rio)Hippie3 (Admin)25 1 11-09-04  01:04 pm
Archive through November 13, 2004Taoist Shredder (TaoGOLLY (Golly)25 1 11-13-04  02:56 pm
Archive through November 21, 2004ohmatic (Ohmatic)rodger rabbit (Skyyp25 1 11-21-04  05:13 pm
Archive through December 02, 2004ohmatic (Ohmatic)CurryShroomer (Curry25 1 12-02-04  09:12 pm
directions for using ionizerHippie3 (Admin)ohmatic 4 1 12-03-04  02:55 pm
Archive through December 09, 2004Hippie3 (Admin)Golly (Golly)25 1 12-09-04  07:13 pm

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Duke X (Dukex)
Senior Member
Username: Dukex

Post Number: 708
Registered: 01-2004
Posted on Thursday, December 09, 2004 - 08:12 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

"*bump*
noone interested anymore? "


Yes just lurking keep up the good work.

Peace...
"Courtesy Of The Red, White And Blue"
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Hippie3 (Admin)
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Username: Admin

Post Number: 30110
Registered: 02-2001
Posted on Friday, December 10, 2004 - 07:45 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

this is ok
but it bears pointing out that now we have many more variables than just the Ionizer's effectiveness in play here.
at this point failure could be caused by many unrelated issues.


Namaste


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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 206
Registered: 10-2004
Posted on Saturday, December 11, 2004 - 09:45 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

allright so the agar has started to 'fluff up'
pretty much everywhere,
but so far it only grew over about 2-3 grains each,
so i guess i will wait till tomorrow before i shake them.

no signs of any funny colors so far.
peace ohm
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Hippie3 (Admin)
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Username: Admin

Post Number: 30191
Registered: 02-2001
Posted on Sunday, December 12, 2004 - 12:30 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

good.
keep us updated plz.
thx


Namaste


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rodger (Skyypilot)
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Username: Skyypilot

Post Number: 4766
Registered: 02-2003
Posted on Sunday, December 12, 2004 - 04:02 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

It will take 48 hours or so for the agar wedge to recover. Then it will take another 48 hours or so to really get 'jumped off' into the grains. The agar will also colonize on the bottom now that it's in a grain jar. Don't be in a hurry to shake the jar. Wait a week or so. I'm not convinced it really helps anyway. Shaking damages the mycelium, which then takes a few days to recover. I'm sure in many cases the setback from shaking outweighs the benefits of spreading the mycelium out. If you'll use three or four pieces of agar in each jar, they'll usually get spread around enough from the initial mixing, that shaking later can be eliminated from your routine. In fact, I rarely shake jars any more after the initial inoculation.
"Whatever it is, that girl put a spell on me". . .jimi hendrix
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ohmatic (Ohmatic)
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Username: Ohmatic

Post Number: 208
Registered: 10-2004
Posted on Monday, December 13, 2004 - 10:12 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

hmmmmm oh well, nice to know that now ;)
i shaked em yesterday after about 10 grains of each jar were infected, so well.

i think i saw a dash of some green in one jar,
but then i shook them all.
anyway, now theyr being indubated and well see.
peace ohm
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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 211
Registered: 10-2004
Posted on Thursday, December 16, 2004 - 08:25 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

some update.

all of the jars have osme nice agar growing,
no fancy colors in ANY of them jet.

and something funny i just discovered and wanted to share.

http://archives.thenook.org/tek/pics/gbox2.jpg

these are old ass pictures from the nooks archives,
do u recognise the little device in there? ;)

the dude also claims never having a contam since
hes been using this box.

interesting eh?
peace ohm
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Hippie3 (Admin)
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Username: Admin

Post Number: 30361
Registered: 02-2001
Posted on Friday, December 17, 2004 - 11:50 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


quote:

these are old ass pictures from the nooks archives,



actually
they're from mycotopia originally.
the nook's archives are mostly
a word-for-word copy of our archives
but two years out of date.


Namaste


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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 214
Registered: 10-2004
Posted on Saturday, December 18, 2004 - 12:06 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

anyway its about the fact the ion's in that pic!

heres some news all of u will love:

i got growth in ALL jars, mycelium growth and not
a single contam so far.
this goes for both, the h2o2 and the 'normal' jars - aint that sweet?
peace ohm
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ohmatic (Ohmatic)
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Username: Ohmatic

Post Number: 215
Registered: 10-2004
Posted on Saturday, December 18, 2004 - 02:36 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

some shots of the nicest jars :

Upload
Upload
Upload

whatcha say :-)

they have all been shaken once btw and i must add
the tamp. grows increeeedibly slow on the birdseed,
even though the water content is aight and their
being incubated :/
peace ohm

(Message edited by ohmatic on December 18, 2004)
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talkingmonkey (Talkingmonkey)
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Username: Talkingmonkey

Post Number: 10
Registered: 11-2004
Posted on Saturday, December 18, 2004 - 03:44 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

any pics of the metal arc that is being used?
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Golly (Golly)
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Username: Golly

Post Number: 326
Registered: 08-2004
Posted on Saturday, December 18, 2004 - 05:18 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Here tis: just 1/2 inch mesh covered with a plastic leaf bag..The thin wire in top left is connected to the grounded scew of a wall outlet..Just did some agar cloning under it so we'll see...
Upload
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ohmatic (Ohmatic)
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Username: Ohmatic

Post Number: 216
Registered: 10-2004
Posted on Saturday, December 18, 2004 - 06:40 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

btw i dont use that dome, straight open air.
peace ohm
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talkingmonkey (Talkingmonkey)
Junior Member
Username: Talkingmonkey

Post Number: 11
Registered: 11-2004
Posted on Saturday, December 18, 2004 - 06:45 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

so what is your concensous golly
as far as your tests go
using the dome
do you think that the ion is a good substitute for a flowhood, and can readily be used for agar work just fine
or do you believe that it is VERY HELPFUL in keeping contams down (as im sure no one will argue) but all in all a flowhood would be a better option.
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ohmatic (Ohmatic)
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Username: Ohmatic

Post Number: 217
Registered: 10-2004
Posted on Saturday, December 18, 2004 - 07:07 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

its handy in keeping them down,
it is NO substitute.
peace ohm
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rodger (Skyypilot)
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Username: Skyypilot

Post Number: 4892
Registered: 02-2003
Posted on Saturday, December 18, 2004 - 07:17 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


quote:

Just did some agar cloning under it so we'll see..



I hope you wore sterile gloves. It doesn't matter what you use, flow hood or not, always use gloves. More junk falls off your hands than anywhere else, so I doubt the unit would be much help since your fingers are so close to your petri dish.
"Whatever it is, that girl put a spell on me". . .jimi hendrix
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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 218
Registered: 10-2004
Posted on Saturday, December 18, 2004 - 08:04 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

thats interesting since i always wash my hands REAL
proper b4 i do any mush work.
i doubt theres much crap on them since they get some
ipa aswell before i get to work.
peace ohm
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rodger (Skyypilot)
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Username: Skyypilot

Post Number: 4894
Registered: 02-2003
Posted on Saturday, December 18, 2004 - 08:11 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


quote:

i doubt theres much crap on them



Take a good hard look at your hands. Every thing you see right now is DEAD. There are no blood vessels in the outer(epidermis) layer of skin. What you see when you look at a naked human body is dead skin cells. Each cell is loaded with bacteria, and just waiting for a new cell to form below it to push it off the body and into the air. I'd suggest you wear latex gloves, rinsed very well with alcohol before you begin any sterile work.
"Whatever it is, that girl put a spell on me". . .jimi hendrix
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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 219
Registered: 10-2004
Posted on Saturday, December 18, 2004 - 08:20 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

very true what u say,
rubber gloves would do the deal aswell though.
peace ohm
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Golly (Golly)
Senior Member
Username: Golly

Post Number: 327
Registered: 08-2004
Posted on Saturday, December 18, 2004 - 09:10 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Yup i agree that most of the contams occuring under the arch are brought there by me. ..I do use gloves and mask for agar+spray down with bleach solution.
The arch itself is just to reduce air currents in the work area.
The metal grid is to attract ionized particals away from the area.
I have not had any contams yet,,, but then this recent agar transfer is the first super sensitive operation so far....I'll let ya know what happens ...
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talkingmonkey (Talkingmonkey)
Junior Member
Username: Talkingmonkey

Post Number: 12
Registered: 11-2004
Posted on Saturday, December 18, 2004 - 09:15 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

golly
were you doing agar work before the ionizer
if so what was your contam rate
it is pretty wild that you are doing agar from prepping dishes to isolation with no contams
very impressive
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Hippie3 (Admin)
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Username: Admin

Post Number: 30441
Registered: 02-2001
Posted on Saturday, December 18, 2004 - 11:34 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


quote:

most of the contams occuring under the arch are brought there by me



that's always the case,
as rodger sez.
wear gloves, face mask too.
suit up like a surgeon if you can,
it works
that's why surgeons dress like that.

Namaste


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Golly (Golly)
Senior Member
Username: Golly

Post Number: 329
Registered: 08-2004
Posted on Saturday, December 18, 2004 - 11:39 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Don't be too impressed yet. I'm so new at agar i don't even know what a prepping dish is
But this is just the next step in the whole Ion testing thread and my education in cloning.
The significance of this thread is if an average Joe like me can get good results then the Tec will likely work for anyone who is not yet prepared to invest in a flowhood.
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Golly (Golly)
Senior Member
Username: Golly

Post Number: 330
Registered: 08-2004
Posted on Saturday, December 18, 2004 - 11:45 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I just realized what you meant by "prepping dishes" DOH..!!!
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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 222
Registered: 10-2004
Posted on Sunday, December 19, 2004 - 11:21 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

i wonder if i should shake the jars u can
see in the pictures above,
because as i said before,
it really takes the mycelium AGES to grow
and the multiple incoculation spots would speed
things up dramatically i think.

whats ur opinion guys?
peace ohm
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Hippie3 (Admin)
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Username: Admin

Post Number: 30472
Registered: 02-2001
Posted on Sunday, December 19, 2004 - 11:43 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

not yet,
they need to grow a bit more first.
i like to see about 25-30% growth thru-out the jar b4 shaking.

Namaste


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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 223
Registered: 10-2004
Posted on Sunday, December 19, 2004 - 11:49 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

damn that will take me several more days if not a
week till this super laid back myc goes so far,
anyway.

is it normal for tampa to grow so slow or is the
isolate i got simply slow?
btw the petri the agar been cut out has been lyring
around for QUITE some time.
peace ohm
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Hippie3 (Admin)
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Username: Admin

Post Number: 30474
Registered: 02-2001
Posted on Sunday, December 19, 2004 - 11:52 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

you already shook them once yes ?
anyway,
the age of the tissue used is relevant,
older tissue is less vigorous
which may result in slower-than-average growth.
plus the seed looks a bit too wet,
which also slows things considerably.


Namaste


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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 224
Registered: 10-2004
Posted on Sunday, December 19, 2004 - 12:00 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

hmm as u can tell from the multi spots its growing
in one jar i MUST have shaken it already once since
there was only one wedge used per jar.

anyway, the somehow wet look comes from the conensation
inside the jar and the water collecting @ top and
running down, i think i got the content figured out pretty well.

anyway, guess ill just let them go on colonise and
shake the jars that have colonised the fewest
in a couple days and just leave the others to go
on colonising.
peace ohm
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Hippie3 (Admin)
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Username: Admin

Post Number: 30477
Registered: 02-2001
Posted on Sunday, December 19, 2004 - 12:11 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


quote:

hmm as u can tell from the multi spots its growing
in one jar i MUST have shaken it already once since
there was only one wedge used per jar.




a simple 'yes' would suffice,
no need to try to make me look stupid
when i'm trying to help
you.

Namaste


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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 226
Registered: 10-2004
Posted on Sunday, December 19, 2004 - 12:17 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

didnt mean to :-(
u got me wrong just wanted to show that to
others so they know why its been shaken once already.

u really got me wrong on that one hip.
peace ohm
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 30481
Registered: 02-2001
Posted on Sunday, December 19, 2004 - 12:25 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

no prob,
i knew you had already shaken,
that's why i phrased it like i did.
anyway,
repeated shaking at such close intervals is just slowing you down.
one really only needs to shake once,
maybe twice on rare occasions.
every time you shake
you risk failure by massive contam
so try to keep it to a minimum.
wait until it covers about 1/4 to 1/3 of the jar
then just shake once.
after that
just wait it out.

Namaste


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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 227
Registered: 10-2004
Posted on Sunday, December 19, 2004 - 12:30 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

aight :-)
peace ohm
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nomoreusmc (Nomoreusmc)
Senior Member
Username: Nomoreusmc

Post Number: 192
Registered: 05-2003
Posted on Sunday, December 19, 2004 - 08:51 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Stupid question, why don't you just leave the jar alone and let it go like a cake, wouldn't the wbs allow natural gaps for the myc to grow?
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Hippie3 (Admin)
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Username: Admin

Post Number: 30501
Registered: 02-2001
Posted on Sunday, December 19, 2004 - 09:09 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

slower to do that...

Namaste


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nomoreusmc (Nomoreusmc)
Senior Member
Username: Nomoreusmc

Post Number: 194
Registered: 05-2003
Posted on Sunday, December 19, 2004 - 09:54 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

right on
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ohmatic (Ohmatic)
Senior Member
Username: Ohmatic

Post Number: 231
Registered: 10-2004
Posted on Friday, December 31, 2004 - 11:37 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

well i am not there right now but had a glimpse at the
jars about, say, 3days back.

they are more or less ALL 100% without one single
jar having anything but nice, white myc inside.

this goes for the peroxide treated and the non peroxide
treated jars.

once again, all jars were unscrewed, lid removed,
wedge thrown in and lid screwed back on in open air
in front of the ion, so if there was something in the
air it SHOULD have hit the jars.

i must say that this is quite badass, and i am
really surprised, if thats not some good news
then i dunno what.

have a good new year.
peace ohm
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talkingmonkey (Talkingmonkey)
Member
Username: Talkingmonkey

Post Number: 32
Registered: 11-2004
Posted on Friday, December 31, 2004 - 07:55 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

ive read this whole thread
and at some points it seems like the ion is NOT good enough
and at other points it seems it IS good enough
is there a general concensous yet? or is more testing needed?
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Golly (Golly)
Senior Member
Username: Golly

Post Number: 356
Registered: 08-2004
Posted on Friday, December 31, 2004 - 09:46 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

My conclusion is that for the money and effectiveness u cannot beat a plain ole glove box...But it does help improve your odds of reducing contams generally ..
If the cash flow is tight then a homemade G.box is the way to go..
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Admin (Admin)
Board Administrator
Username: Admin

Post Number: 31155
Registered: 02-2001
Posted on Monday, February 07, 2005 - 03:43 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


quote:

it does help improve your odds of reducing contams generally



that's the consensus

Namaste


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