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Hippie3 (Admin)
Posted on Wednesday, November 13, 2002 - 01:23 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

from una of mushmush at shroomery.org
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Grass seed (2 liter) and water (1 liter) combined in a polyproylene bag.
1

Bag is pulled thorugh a polypropylene ring (cut from a big syringe)
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Bag is folded back...
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...and secured with two rubber bands
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The opening is stuffed with polyfill.
5

And this is covered with aluminium foil to prevent it from getting wet in the pressure cooker.
6

Bags after autoclaving (2 1/2 hours)
7

These bags should be inoculated with liquid mycelium since they cannot be shaken.
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Hippie3 (Admin)
Posted on Wednesday, November 13, 2002 - 01:30 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

now, as is, his method is aimed at producing sclerotia, hence the grass seed.
but this could easily be adapted to other grains, not filling the bags so much so they can be shake, etc. improvise upon these good ideas.
more ideas-
Rather than using polyfill, if you can find a large Tyvek mailing envelope? Simply, cut it up into squares or circles & use it as a gas permeable filter in place of the polyfill, held in place with rubber bands. Tyvek will withstand autoclave or PC temp's & works well as a filter.

Also, at most hardware stores, one can find high temp plastic hot water plumbing PVC pipe in diameters from 1/2 to 2 inch. They come in 8 foot lengths & are very inexpensive. You can simply hacksaw small lenghts to use as a ring neck for the bag top. That way you can make as many rings as you wish & bigger size than a syringe, for very little $$.


Ordinary oven bags will work. Be careful the side of the bag doesn't touch the pressure cooker or the bag may still melt. If your PC is small, you can use cotten (no nylon!) string to tie the bag into shape. test each bag for leaks beforehand by filling with water.
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Hippie3 (Admin)
Posted on Wednesday, November 13, 2002 - 01:32 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

more from una.

Quote:


Instead of using PVC pipe i used special cardboard rings that withstand autoclaving.
Inoculation was done with 50 ml of mycelial suspension injected with a syringe through the tyvek filter. The hole was taped.



8

Quote:

Here's a picture of an (almost) fully grown bag, two weeks after inoculation. Some small scattered sclerotia are already starting to form.



9
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Hippie3 (Admin)
Posted on Wednesday, November 13, 2002 - 01:33 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

archive material.

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