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Mycotopia Web Archive Archive Non Cubie Shrooms Wild strain of Paneeolus cyanescens Previous Next

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Strangeland (Waylitjim)
Senior Member
Username: Waylitjim

Post Number: 390
Registered: 06-2004
Posted on Sunday, February 06, 2005 - 03:33 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

This is a wild strain of Panaeolus cyanescens cultivated by Una in the Netherlands.

Substrate is cow dung/straw/vermiculite sterilised in a filter patch bag.
This was spawned with rye spawn and incubated for 10 days at room temp.

A shallow casing layer of peat/limestone/vermic. was applied and after 5 days the top part of the bag was cut away and the bag was placed in a fruiting chamber. Pins appeared 6 days after.

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(Message edited by waylitjim on February 06, 2005)
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Strangeland (Waylitjim)
Senior Member
Username: Waylitjim

Post Number: 391
Registered: 06-2004
Posted on Sunday, February 06, 2005 - 03:49 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

For comparison sake, here is Panaeolis tropicalis by Olgualion:
Millet > pasteurized horse manure.

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Troutlips (Troutlips)
Senior Member
Username: Troutlips

Post Number: 379
Registered: 02-2004
Posted on Sunday, February 06, 2005 - 03:52 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Oh Mama! What a flush!!!

Not a trained professional. Please try this at home.
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 31189
Registered: 02-2001
Posted on Monday, February 07, 2005 - 09:59 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

very nice
archive material
any other details on methods used, etc. ?

Namaste


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neverblink (Dial8)
Senior Member
Username: Dial8

Post Number: 260
Registered: 12-2004
Posted on Monday, February 07, 2005 - 10:02 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

holeeeeeeee shit. How long from inoculation to fruit?
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Strangeland (Waylitjim)
Senior Member
Username: Waylitjim

Post Number: 395
Registered: 06-2004
Posted on Tuesday, February 08, 2005 - 01:37 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Explanation by Olgualion:

"This jar of colonized birdseed was used to spawn
a tray of microwave pasteurized horse poo.

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"The dry manure was broken up and placed in a microwave safe bowl. Next he adds boiling water to the bowl. It doesn't matter if you add too much. this is allowed to sit for a half hour or so. He then puts the bowl in the microwave for like 5 minutes or so on high. removes the bowl and mixes up the dung. Puts it back into the microwave and nukes for a final 8 minutes on high. As soon as it stops he places some AL foil on top of the bowl, but leaves it to cool overnight.

In the morning, he washes out an aluminum pan and begins squeezing the dung to remove much of the water and puts it into the pan. After he has it all squeezed out, he spawns the poo with Panaeolis tropicalis on wild bird seed.

Knowing your strain or isolate is very important. If you have a strain that shows too much variability with multispore, then it is important to make an isolate. Proper maintenance of the tray before initiation is very important. Water content and patching are most important now. Dude keeps his casing often beyond field capacity, but always fans vigorously after a spraying. You want to patch any areas of mycelium that are dense to achieve an even pinset. This tray was left in the fridge for 2 or 3 days due to limited room in the fruiting chamber"

When initiation starts, I believe consistent air exchange is the most important factor. This is why dude leaves his coolmist on 24-7. He doesn't want to worry about the humidity, at the expense of air exchange. So he keeps the air exchange constant and to reduce humidity he randomly places holes around the terrarium. He found that they dont like a high humidity after the pins have formed. They grow more natural looking and tall at 80% RH than at 90 or even more."




(Message edited by waylitjim on February 08, 2005)
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Strangeland (Waylitjim)
Senior Member
Username: Waylitjim

Post Number: 396
Registered: 06-2004
Posted on Tuesday, February 08, 2005 - 02:48 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

This is the Australian Pan cyan by Anno:

90% straw, 10% dung, sterilised, spawned with millet, casing 10 parts peat,
10 parts vermiculite, 10 parts coco coir, 1 part calcium carbonate, water.


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"For me, the sterilized dung contaminates less than the pasteurized.
The downside is that the sterilization and innoculation of big amounts
is technicaly more requiring.

When you pasteurize, you can inoculate the substrate more or less in the open,
since the pasteurized substrate has some resistance to contamination.

If you sterilize the substrate, you HAVE TO inoculate and colonize it
in sterile conditions, ie inoculate in front of a flow hood and colonize in a spawn bag for instance.

If I personally want to do small amounts of substrate, then I sterilize,
if I have bigger amounts, I pasteurize."

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Sharkie Jones (Rainbowfungus)
Senior Member
Username: Rainbowfungus

Post Number: 829
Registered: 12-2002
Posted on Tuesday, February 08, 2005 - 04:04 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

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That's an amazing flush. Congrats.
God bless the Post Office, and tyvek.
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Mushit (Mushit)
Member
Username: Mushit

Post Number: 22
Registered: 03-2004
Posted on Tuesday, February 08, 2005 - 04:47 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

MAN!! I hope when I try them they will flush like that!!!
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El Mas Ingorante (Pedro)
Member
Username: Pedro

Post Number: 21
Registered: 01-2004
Posted on Tuesday, February 08, 2005 - 12:58 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

very nice genetic!

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