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Nan's Tips & Teks - 1
Clean Karo Syrup Tek - 1

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Timothy Leary (Timothyleary)
Posted on Monday, October 08, 2001 - 03:48 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I was wondering if it is possible to clone in honey water. or if not clone(using actual tissue), then could one possibly take a small portion of mycelium from a cake, drop it in a sterlized honey/h2o solution?

i apologize if this or simliar questions have been ansered before. I have not seen any in the recent posts (last month or so).

Any thoughts are welcome, but simple yes/no answers with no explinations are fine if no one has the time.

Thanks,
TimothyLeary
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Nanook of the North (Nanook)
Posted on Monday, October 08, 2001 - 06:53 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

It's OK. It's a good question.

The way I do it is to take a tissue sample from the stipe and blend it per 9er Tek. Then I shoot jars of liquid substrate, grow it out 3 days at 87*F in an incubator, and use the liquid inoculum to shoot jars. The liquid inoculum can be stored for up to one year in the fridge.

Nan
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Timothy Leary (Timothyleary)
Posted on Monday, October 08, 2001 - 05:09 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Let's think about this:

If you could use a piece of mycelium in honey water, every jar can make a whole bach of honey water without compromising its fruiting at all.

One batch of honey water per jar, ten jars in one syringe, one syringe would last you along time.

I think i should call it the super poor man's tek. Unless there is a tek like it? if not, i am starting experiments.
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Marx2k (Marx2k)
Posted on Wednesday, October 10, 2001 - 07:54 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Also, you can add up to 5ml Hydrogen Peroxide AFTER sterilizing the honey water to ward off any contaminants that may be lurking around.

Shroom Glossary