myco domesticus (Mycophil)
|Posted on Thursday, May 15, 2003 - 11:46 pm:||
with thanks to sixtango at the shroomery
6Ts Grain to Grain Transfers (G2G)
Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.
The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.
Doing G2G in a Glove box and/or in front of a flow hood is preferable. But, you can still manage under aseptic conditions, without them, if you are very CAREFULL.
Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you dont have one. You can usually manage without it
Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.
Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about ¼ of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting it’s filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.
Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3 recuperate from that shock. There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.
Colonizing G2G jars
Colonizing G2G bags
EDIT......OOP's, last pic is a grain transfer to a bag of compost, not grain.
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
and a replie from poke smot :
6T might I add, you should shake the jar you are using (colonized) 12 to 24 hours before doing the transfers. This gives time for the mycelium to re-establish itself and colonize the surface area of the grain.
When you are ready to do the transfer, shake the jar lightly to break it up, without disturbing it too much.
IMO if your workspace is clean enough, you don't need to take extreme precautionary measures. I know of people who don't use lysol, alcohol, etc when doing g2g, and he does fine using those to spawn straw or whatever shit he has.
If you're into mycology, get some petris and some MEA or PDA, and pour your own dishes. Then work from multispore from your favorite 6" sporeprint, and isolate a virile strain. Once this pure strain is isolated, a wedge of agar can be transferred to a single quart of grain. This takes a little while to colonize, and a FOAF usually does batches of 12 pints, each using different isolates. Whichever grow in first are used; some have colonized in a week, shaking every 2 or 3 days.
Since there's less rye that small colonized agar slice will colonize the rest of the rye fairly quickly. From there you could spawn 6 quarts with 1 pint of your favorite isolate; here is where you transfer a small sample of colonized grain to an agar slant if you didn't do that off the original isolate.
Those 6 quarts can spawn more via. g2g. For fast colonization, I would go with 1 quart to 10 quarts. As such you could go to 60 jars, or use each quart to spawn a large mycobag consisting of 10 quart's worth of rye grain.
Rye is an OK fruiting medium, but it is also very good to use to spawn straw. Having individual colonized grains allows many inoculation points, if you're spawning straw or shit or wood.
Hope that run-down was informational and accurate
|Posted on Thursday, May 15, 2003 - 11:53 pm:||
very nice, thanks.
myco domesticus (Mycophil)
|Posted on Friday, May 16, 2003 - 12:20 am:||
when i saw it , i figured it could be something for the new archives , i don't remember many postings on g2g's in the archives ,personally i just shake from jar to bag or bag to bag lately in front of a flowhood , no contams yet but shiitakes seem a rather agressive specie , even with a lot of flux in the temps , they still continue to grow in the growbags but i wouldn't recommend my way to someone ;)
|Posted on Friday, May 16, 2003 - 12:52 pm:||
there's a bit in the archives but this is certainly a great addition, well illustrated.
6T is very good.