|Posted on Tuesday, June 24, 2003 - 12:45 pm:||
as to taming a wild spore print.
this is nothing new.
the steps are quite basic and you're only going to run into problems if you stray too far off the beaten path.
first, you assume the spore sample is contam'd.
of course you make every effort to get a clean a print as possible, but under field coinditions it's pretty safe to assume some contams will get in.
so the first thing you must do is germinate it on a solid media.
folks use agar for a very good reason, namely it's not very appetizing to micro-organisms, and they are much slower growing on agar than otherwise.
one can use half pint jars as an acceptable substitute for petris but there is no good substitute for agar.
once the spores germinate on the agar, all work being done in a sterile environment from this point, one snags a tiny bit of mycellia from the cleanest best looking part of the petri and transfers that to some antibiotic/peroxidated agar.
the antibiotic/peroxide will help to further clean up the colony as it grows out.
then, you repeat the process, again snagging a good looking piece of rhizo mycellia for transfer, you want it as far away from any contams that might be present on the petri as possible.
the process of growing it out, and transfering, is repeated until you get some that grow out 100% clean.
yopu now have your basic culture from which you can procede to domesticate the strain.
to further select for better fruiting/growing substrains, begin growing out your new cultivar on various substrates and select the best examples to clone and propagate further until you get where you like what you see.