I feel so weird (Phishgrower)
|Posted on Friday, October 19, 2001 - 07:42 pm:||
Ok, I understand everything about this tek, except for how to keep the jar lids during the process. Do you just leave the hole in the lid open after you innoculate or do you cover with tape? Leaving it open would introduce bacteria, would it not? please help, thanks.
|Posted on Friday, October 19, 2001 - 07:52 pm:||
just put some tape back on.
|Posted on Friday, October 19, 2001 - 07:58 pm:||
I put the jar in the Glovebox and just take the lid off. No holes at all. After I shoot a jar I put a clean, dry, sterile, solid dome lid with no holes, seal side down, over the mouth, screw the band down tight, and replace the foil cap. I presterilize some solid dome lids wrapped in foil when I sterilize the jars. That's a mouthfull...
People use Polyfil, but I will tell you what, it does not work well for liquid culture. Sugar solution inevitably gets into the polyfil, and if a contam starts here it's all over... It will grow through the polyfil in many cases and drop spores inside the jar. Cobweb is especally good at this and it's hard to see, even in dextrose (very tiny black sporebodies)... Ever shoot 30 jars of cobweb?... What a mess... You could easily end up shooting yeast as well without knowing about. Polyfill works great for shooting Grain Tek and 9er Tek. Not good for liquid culture.
In liquid culture you should use solid lids: no holes, no tape, Incubate and store with the Foil Covers on. Keep em clean. Experience talking. Follow the Tek until you are comfortable with honey/dextrose.
|Posted on Thursday, December 13, 2001 - 06:35 pm:||
Ok, I've tried my dextrose jars in many things. My first batch was in an extra 250mL jar (which come only "decorated" around here, making them hard to see through) and it was a pain because I couldnt nuke the jar w/ its lid on, and saran wrap didnt hold while shaking the water about.
My second attempt was in an Absolut Vodka bottle, a 200mL one, worked ok, but the stuff hardly grew for some reason.
My next attempt was in ziplock 1/2 pint containers, they worked well, at first, but they are all warped now from heating to sterilize them (never take the lid off a pc to cool when using these, they shrivel like a frightened turtle) plus the seal wasnt the greatest and my last two were contamed somehow.
My latest attempt was in baby food jars, but that resulted in some sediment, which I now believe was rust off the lid.
So now I need to make up more, since my jars are all almost ready to birth. Any ideas of what I can use for containers?
|Posted on Thursday, December 13, 2001 - 06:44 pm:||
Brad, i just use a pint size mason jar with an inoculation hole. works great.
|Posted on Thursday, December 13, 2001 - 06:49 pm:||
They would be decorated too
|Posted on Thursday, December 13, 2001 - 09:11 pm:||
fungi perfecti has plastic autoclavable lids for your jars, perfect for microwaving dextrose water.
just search their catalog for 'plastic lids'.