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Mycotopia Web Archive Archive Liquid Culture: Karo/Honey/Dextrose Q&A Honeytek Easy No PC Needed HONEY TEK Previous Next

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Topic Author Last Poster Posts Pages Last Post
Liquid Culture - 1
EZ HoneytekNanNan1 2 10-26-01  03:16 am
No luck with Honey!BobbyBobby10 2 10-24-01  02:45 am

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Nan (Nanook)
Posted on Tuesday, October 23, 2001 - 06:24 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Posted by Hippie3

Materials

1/2 pint wide mouth jar
bottled water
1 tsp honey
saran wrap
rubber band
masking tape
microwave oven
2 cup microwave safe measuring cup
Sterile spore syringe
Disinfectant such as Lysol or bleach water in a spray bottle
Pot holder or oven mitt

Procedure

Have all materials at hand so you can work quickly.
Put about 1 1/8 cups water in the measuring cup.
Add about 1 tsp honey to the water and stir until dissolved.
Place the cup and the half-pint jar in the microwave.
Fry on high for five minutes
Open the microwave do not remove the cup and jar.
Working quickly within the microwave and using the potholder to protect your hand, pour water into the jar to about the bottom of the threads. Stretch saran wrap over the top of the jar and secure with a rubber band.
Let cool to room temperature.
Clean a working surface such as a table with a disinfectant such as Lysol or bleach water.
Mist the surrounding air with the disinfectant.
Working quickly inoculate the jar through the saran wrap with a sterile spore syringe using about 2 cc and cover hole with masking tape.
Incubate at 80 degrees Fahrenheit.
Once the mycelium has grown, shake to distribute the mycelium, and poke thru the saran wrap with a sterile syringe and suck up the white mycelium, cover the hole with tape and save for further use.

p.s. one might substitute tyvek for saran wrap.
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Brad (Raze)
Posted on Saturday, November 03, 2001 - 07:56 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Hey, I'm doing your honey tek for the second time now. The first had black dots in it, so I decided not to risk using it. After about 15 days or so it stalled anyways.
The first time I slapped saran wrap on, then my lid ontop (using 250mL canning jars) but this time I'm doing it exactly to your tek.
Right now its cooling, and I've noticed what I think could be a tragic flaw (but then again, it could be fine). The jar is cooling, and so is the hot gas inside, which compresses as it cools, now that's caused a suction, the saran wrap is being sucked down quite a bit, looks like an inverted bubble. But if I listen carefully, I can hear a noise, and I'm 90% sure its air being sucked in from outside. I have it held on tight with an elastic, but air is a sneaky bitch, and she'll find a way in.
Is this normal with your tek? Or did I do something wrong? Because having unclean air sucked in sounds to me like a major contam issue.

Thanks
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Nan (Nanook)
Posted on Saturday, November 03, 2001 - 11:56 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

There is material down there which orginiated from the archives of the old board which I have never tested. I presume you are referring to the No PC Honey Tek?

What do you think guys... Should I pull the Tek?

My liquid tek is posted: Nanook's Karo Tek

The lids in my Tek equalized properly in the PC

Lichen, how has your experience with the No PC Honey Tek been?
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Lichen (Lichen)
Posted on Sunday, November 04, 2001 - 12:41 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

good question. It has not developed beyondan 1/8 inch cloudy substance in the bottom of the jar. I innoculated several jars with it a few days ago, and cannot report any growth yet. Time will tell; if in a few days it doesn't grow, I will have to say I'm dissapointed. BUT-- there aren't any black specs in it, just cloudy, creamy-looking stuff. Doesn't look like it has contammed yet, Nan. I wouldn't pull that tek just yet, let it go a few days and we'll see. The innoculum is only in its sixth day of incubation.

Nan, I want to reiterate. I used the tek you posted where you scrape up, squirt water into, and suck up the mycelium from the bottom of the freshly-birthed jars. It's working very quickly, faster than anything else I'm doing, and no signs of contams yet. This is good, because it gives me another chance to save my B+, which is on its way out if I can't be mycologist enough to save it.
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Nan (Nanook)
Posted on Sunday, November 04, 2001 - 01:03 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I agree those syringes are about the bestest
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Lichen (Lichen)
Posted on Sunday, November 04, 2001 - 01:09 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

So instead of starting a new thread on the subject, I will post it right here and hope for the best.

Fanaticus has posted that he has had to drop the Albino race because the spores are so scarce. Yet I intend to print this race. So a question: can I get albino spores from the brown-capped shrooms that grow up with the white ones? I am assuming the pure white shrooms do not have any spores...fanaticus said they are sterile. Can anyone help me on this?
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Nan (Nanook)
Posted on Sunday, November 04, 2001 - 01:39 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Yep that is the only way to print them. The pure albinos don't print. This is a strain where you probably want to clone a non-albino shroom that you know will print just as a backup in case you have to get spores.
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hippie3 (Hippie)
Posted on Sunday, November 04, 2001 - 02:55 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

as to the no pc honey tek,
you might be better served by using a big wad of polyfil instead of, or in addition to, the saran wrap...so any air entering would be filtered...
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Brad (Raze)
Posted on Sunday, November 04, 2001 - 03:36 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Well, I"m gonna let this sit for a few days w/o innoculating it, just to make sure they honey is fine.
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Brad (Raze)
Posted on Monday, November 05, 2001 - 01:42 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Hmm... two days and mine already looks like my last one at two weeks... except I havent innoculated this one....
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Nugdumper (Nugdumper)
Posted on Monday, November 19, 2001 - 03:41 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Quick question regarding Hip's Microwave Honey Tek, found at:

http://archives.mycotopia.net/discus/messages/5/3035.html?1005204811

1. How much h202 would one need to add to this solution, and when? At the first sign of growth??

2. How long can you keep a honey/water culture before its no good to use?
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Lichen (Lichen)
Posted on Monday, November 19, 2001 - 03:47 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

where did you get H2o2 in the equasion?
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Martaxus (Martaxus)
Posted on Monday, November 19, 2001 - 06:48 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

H202 is to be injected roughly 30 min before drawing up syringes. I think it's just a few ml's, exactly how much I don't know.

Preservation of grown inoculum is helped by refridgeration(NO freezing!). Over a month anyway...anyone know?
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Nan (Nanook)
Posted on Monday, November 19, 2001 - 07:22 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Six months for refrigerated liquid inoculum here and it still shoots like fresh.
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Nugdumper (Nugdumper)
Posted on Tuesday, November 20, 2001 - 05:51 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I thought you added h202 2-3 days into growth?
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Nan (Nanook)
Posted on Tuesday, November 20, 2001 - 06:08 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Depends on the Tek. H202 is lethal to spores, but you can shoot a few cc's H2O2 per 100 cc of liquid inoculum any time after the spores germinate.

Really, if everything is clean you do not need H202
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Nugdumper (Nugdumper)
Posted on Tuesday, November 20, 2001 - 08:49 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Thx Nan. I'll be doing two half-pint cultures. Think I'll be able to shoot 40 jars in a week?
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Nan (Nanook)
Posted on Tuesday, November 20, 2001 - 03:59 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Follow the Tek, spore germination plus enough time for the culture to grow out to the point where you can shoot jars is typically 7-14 days.