  
Nan (Nanook)
| | Posted on Tuesday, October 23, 2001 - 07:00 pm: | 
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Captain's Oven Technique
OK OK... Here is the real poop: Oven Tek Sux. The flow dynamics theory is wrong. The best place for sterile work is in a Glovebox. You can shoot jars in a clean still room. I left Oven Tek here because the tek is too firmly entrenched to remove (see links below) but none of the staff here uses it nor recommends it. Period. - Nan
Karna's Syringe Tek : Simple Cubie Tek : Lazlo's Syringe Cloning Tek : Spore Print Question : Inoculation Discussion : Psilocyber's Syringe Tek : Dr. Stein's Syringe Tek : Growing Pan Cyans : Inoculating with Filter Disks : Camel's "Advanced" Method : Yeast : Shroom Glossary |
Mr. B (Argonaut)
| | Posted on Tuesday, December 04, 2001 - 05:08 pm: |
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I have invested enough into my current setup so I really don't want to fuss with a glovebox. Can I get opinions on the open oven-door method as opposed to glove box??
:-) |
jared (Jared112)
| | Posted on Tuesday, December 04, 2001 - 05:44 pm: |
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It is suppose to work good enough if you don't want to spend the money. I never tried it, but I know that when it comes time for me to work with spore prints I'm going to make a glove box. Here is a pic of a very nice and cheap glovebox that Lichen made.
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hippie3 (Hippie)
| | Posted on Tuesday, December 04, 2001 - 05:57 pm: |
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here's photo proof the oven method is not
reliable-
note where the smoke goes...
where smoke can go, contams can too.
Build the Cheapest "Glove" Box Possible
Items Required
Cardboard box (13"x15"x10")
Plastic wrap
Duct tape
Dish cloth
Disinfectant spray
Sharp knife or razor blade for cutting cardboard
Rubbing alcohol
No gloves
To raise the odds against contamination, a sterile work area is vital. This is especially true if you want to become independent of outside sources of sterile spore syringes. In a rush I threw together this glove box out of scrounged materials. It has worked so well that I haven't built my dream box yet. First find a clean cardboard box with the above dimensions as a minimum. It doesn't have to be new or pristine, just fairly new with no visible dust or dirt. Cut off the top opening flaps. Cut away the entire front of the box, leaving a 2 inch wide strip of cardboard at the top edge to attach the dish cloth to. Cover the top with plastic wrap and tape down the edges. Using duct tape, attach a dish cloth or towel across the front opening onto that 2 inch strip. The cloth should cover the entire opening of the front of the box like a curtain and you should be able to see into the box through the clear plastic wrap on top. That's it. To use the box, spray down your work surface with the disinfectant spray. Close off all drafts (windows, heater fans, etc). Spray outside of box lightly and saturate the dish cloth. Place bulky items into the box such as grain jars and agar plates. Spray disinfectant into the box until all of the air inside is displaced by the mist. Wash hands with rubbing alcohol and you are ready to work
(Message edited by admin on May 27, 2004) |
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