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sprout roots dao (Sproutlocs)
Posted on Friday, August 22, 2003 - 08:26 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I got a bag of equ. strain spawn from a friend and it was in a poly bag with a gas exchange patch and a syernge patch. i put the spwan into the incubator and it produced three or for mushies befor the green mold appered. I continualy knocked down the mold and it grew about ten more and i made six prints.
The prob is that four out of six grew mold in the jars Ive moved to a defernet place and tryed yet another set of jars and the mold still grew and all ways wait to see if the pc prosses worked I also sacrifice one or two jar to check moister and oder. the mold in the jars is not green but i'm sure that it is the same fromm the grow box.

WHAT CAN I DO TO SAVE THE OTHER SPORE PRINTS?
MAYBE AGAR PURE CULTURE WILL WORK AND WHAT ARE THE CHANCES OF GETTING ANY GOOD CLEAN SPAWN FROM THIS?
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Hippie3 (Admin)
Posted on Friday, August 22, 2003 - 08:51 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

ever heard of peroxidated agar ?
a thick streak of spores on p.agar and you could clean up those prints.
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Miraclet (Miraclet)
Posted on Friday, August 22, 2003 - 09:37 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

"Many people seem a bit confused as to what peroxide can and can not do. Hydrogen peroxide will kill bacteria and fungal spores and inhibit the growth of yeasts. Unfortunately, mushroom spores fall under the category of fungal spores, so they will not germinate on peroxidated agar because they are killed on contact."

(From the archives)


I know yer smokin the good shyt now Hip...hehehe...But I know what ya meant bro..


"sprout roots dao (Sproutlocs), start yer spores on MEA-Then transfer to perox. agar culture plates....Thats what Hip ment..hehe..Pass it my way...PLEASE!!!!!
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Hippie3 (Admin)
Posted on Friday, August 22, 2003 - 10:08 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

actually i've recently become aware of the fact that you CAN sprout spores on peroxidated agar if you put a heavy enough streak of spores down.
the peroxide kills quite a few but in the process is itself consumed so spores that survive can germinate in the now peroxide-free zone and then the mycellia can grow out across the rest with no contams.
try it.
a nice thick spore deposit in the middle.
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sprout roots dao (Sproutlocs)
Posted on Saturday, August 23, 2003 - 12:07 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

OK but what is this MEA- and what is the perox formula
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sprout roots dao (Sproutlocs)
Posted on Saturday, August 23, 2003 - 12:11 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

oh ya and souldyou wait any time for the H2O2 te evaperate any before you inoculate?
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sprout roots dao (Sproutlocs)
Posted on Saturday, August 23, 2003 - 12:16 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

also do you know if there is a minimum amout of H2O2 that can kill the meany greenies yet not hharm the spores
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Miraclet (Miraclet)
Posted on Saturday, August 23, 2003 - 12:26 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Things that make ya go Hmmmmmmmm..Interesting indeed Hip..Skipping the waiting period through MEA would certainely have it's advantages...

This method would be a wise choice with spores such as "sprout roots dao (Sproutlocs)" may be working with, or even say wild/ or very questionable prints...Ya live, ya learn...But pass the pipe anywhoo
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sprout roots dao (Sproutlocs)
Posted on Saturday, August 23, 2003 - 05:24 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I mean I already have many PDY agar recipes but how do you apply the H2O2?
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Hippie3 (Admin)
Posted on Saturday, August 23, 2003 - 11:36 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


Quote:

Quote:
"First you need to get a hold of some light malt extract powder and some agar agar. Malt extract can be found at your local wine and beer brewing store, and agar agar can be bought from Fungi Perfecti, among other places. You also need some petri dishes, also available from FP, or you can pour your agar into empty jars instead of plates if you prefer. Next, mix three grams of malt extract and three grams of agar (or, if you bought them pre-mixed, six grams of the mix) with 149 ml of water (you should use 149 ml instead of 150 because you want to account for an extra ml of peroxide that you will be adding later). I measure out my water with an empty syringe, keeping in mind that one cc is the same as one ml. I mix and sterilize this solution in a half pint jar, because the amount of agar solution is right for that size jar and because the size of the jar fits well in the pot that I use for sterilizing it. It works for me, but it is not ideal for pouring and I'm sure a better substitute could be found. Anyway, close up your container of agar and sterilize for 45 minutes. I sterilize mine by letting it sit in boiling water in a covered pot, but you could use a pressure cooker if you wanted to. While it is sterilizing, thoroughly clean your plates if you haven't already done so, and stack them on a tabletop. As soon as is possible without burning yourself, move the jar of molten agar to the tabletop, open the lid, and insert a clean thermometer into the agar. Since hydrogen peroxide decomposes at 140 degrees fahrenheit, you need to let the temperature of the agar drop below that before you add the peroxide. Otherwise it will decompose into water and oxygen and be effectively useless. This is why the peroxide is added after sterilization. I have found that my agar begins to solidify at around 120 degrees fahrenheit, so fill a syringe with peroxide and when the temperature falls to 130 degrees, add one ml to the molten agar and stir it in with the thermometer. Then remove the thermometer and pour your plates, stacking them as you go. I can usually pour nine or ten plates with this amount of agar. If you want to make a larger or smaller amount, simply adjust the amounts of water, agar, malt, and peroxide, keeping all the ratios the same."


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sprout roots dao (Sproutlocs)
Posted on Saturday, August 23, 2003 - 03:48 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

thax for the info about the MEA tek and could you tell me how use h2o2 with the pdy agar medium.
and can pdy w/ h2o2 be used in place of the mea
or should i say what is the signifigance of using mea first?
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Hippie3 (Admin)
Posted on Saturday, August 23, 2003 - 03:52 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

the amount of peroxide would remain constant ,
roughly 1-7 ml per 150 ml total volume.
i don't think it matters much if you use pdy instead of mea.
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sprout roots dao (Sproutlocs)
Posted on Saturday, August 23, 2003 - 04:02 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

cool thaz ya berry much loox like i gotz some work to do expielly if spore magic don't come through considering they got my money and if i get burnt this is my only opion


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Hippie3 (Admin)
Posted on Sunday, August 24, 2003 - 05:36 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

good luck.

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