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Admin (Admin)
Posted on Thursday, August 23, 2001 - 11:34 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

3M's "No Shake" Easy Grain Tek

What you are going to need


-1litre autoclavable bags available at any mycology supply store. You can also use instrument sterilization bags from a science supply store


-wheat


-Pressure Cooker


-Polyfil


-electrical tape


-cotton balls and 95% alcohol


Preparing The Bags


Step 1: Cut the Polyfil into 1"x18" strips and set aside for later.
Step 2: Roll the bags down like a sock and place 250 ml of wheat and 175 ml water in the bags.
Step 3: Unroll the bags and take a strip of Polyfil. Roll the strip into a roll approx 2" in diameter and place inside the neck of the bag. Use electrical tape to close the bag. Make it tight enough to reduce the polyfil to approx 1"
Step 4: Pressure cook for 2 hours at 250 degrees F or 15 pounds


Innoculating The Bags After They Have Cooled


Step 1: Cut strips of electrical tape approx 1" in length.
Step 2: Select an area approx 1" below the polyfil plug and swab it down well with a cotton ball soaked with alcohol.
Step 3: Flame and quench a needle, with alcohol soaked cotton ball, and insert it in the center of the swabbed area being careful to ensure that you do not puncture the backside of the bag. Squirt 1 cc of spore solution into the bag.
Step 4: Withdraw the needle and place the precut tape over the hole. Try to make sure that the area of the tape that is actually covering the hole has not touched your finger.


Incubating


Step 1: Place the innoculated bags into a cupboard, box or some other dark place. Try to maintain a temp of approx 80 degrees F or 27 degrees C.
Step 2: When colonization reaches 25% (approx 4 or 5 days after spores germinate) manipulate the grain making sure to break up the colonized grain. Allow mycellium to recover and finish colonizing


*Note: be sure to allow airspace beween the bags of grain. A condition known as thermogenesis will cause the temp of the grain to be higher then the ambient air. If the bags are too close they can exceed the thermal death temp and your cultivation efforts will be in vain
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Krash (Krash)
Posted on Wednesday, October 31, 2001 - 01:47 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

whats up everyone. My job gives me access to a unlimited amount of wheat. I was wondering if I could use this to my advantage. Does wheat grain work better instead of BR, or not?
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Nan (Nanook)
Posted on Wednesday, October 31, 2001 - 01:55 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Wheat will work, but it is high in a protien called gluten which makes it sticky. I would not use wheat flour in the place of Brown Rice Flour (BRF).

Whole wheat berries could be used, but again it's a bitch to work with because the gluten makes it a sticky clinging mess. You have to soak the wheat, cook for 30-40 minutes in a pot of water on the stove, then fluff it up before filling jars a-la 9er Tek... But it does not work well because it clumps and sticks. You have to pressure sterilize just like rye. Mycelia does not colonize it as readily as say whole grain rice or rye which fluff up much nicer, providing a loose porus substrate.

Grain is cheap. I would use something better, wheat is a mess to work with.
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Krash (Krash)
Posted on Wednesday, October 31, 2001 - 03:34 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Thanks for the advice. I think I'll stick with the good old Brown Rice. Thanks for your input.
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relic (Relic)
Posted on Wednesday, October 31, 2001 - 05:07 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

i've never used wheat, but, had heard from karl brooks that he liked it better than rye. i'd never heard of the sticky clingy stuff. if you've worked with it nan, then you should know. perhaps he used that stuff you mix in to keep the grain shakeable, forget the name now. in case anyone has some and wants to use it whole, he said that you don't need to sterilize as long as rye, 60 min. should work. and you can get that shakable stuff at home brewery suppliers if you can remember the name
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Nan (Nanook)
Posted on Wednesday, October 31, 2001 - 05:31 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Gypsum, and it would help. Wheat works as long as you can keep it from clumping up, mine clumped and I did not know about gypsum at the time I was messing with it.

That was also before I learned about phone books and beating and shaking jars while they are hot to keep the grain from forming a solid mass... As long as the grain substrate is porus and you are using enough liquid inoculum to percolate through it, it is OK if things are a bit clumpy. Also you can pre-cook... As in 9er Tek Substrate.

I think if I was messing with wheat again I could get it to work.

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