Black Star (Mr_Bug)
|Posted on Wednesday, October 10, 2001 - 05:45 pm:||
what's a glovebox?
Nanook of the North (Nanook)
|Posted on Wednesday, October 10, 2001 - 06:24 pm:||
9er Tek Glovebox, I also like Relic's Box. Both are cheap. Storage containers make great gloveboxes. A recent addition to the list of Teks is the good Dr.'s Pressure Box which I think is outstanding if you have the $$ and want to invest in this hobby.
A glove box is a Sterile transfer chamber where Jars, Petri Dishes, etc. can be manipulated without Contam issues. It is required for Tissue Cloning and Honey/Dextrose/Karo/Malt Liquid Syringe Teks. You should use a box when making up Spore or Mycelial Syringes, Spore Printing, or tissue transfers of any type.
A good comfortable box means much less stress and better results. See OT's Jar Tek to understand the luxury of a Flow Hood...
The 9er and Relic Teks (both exemplary) use a large clear plastic storage container with some holes cut for the arms. The 9er Tek uses a filtered fan: mounted in such a way as to provide clean Positive Pressure. It uses plastic trash bags for the "gloves".
I built one, found the trash bags "gloves" difficult to work with and made some modifications. Read 9er Tek if (when) it ever comes back up, but note the instructions and modifications below... You should be able to duplicate my box from these text instructions.
I purchased a large clear plastic storage container and opaque lid. The container measures aprox. 3 feet by 20 inches, it is a foot tall. I would not get anything smaller.
After reading 9er tek I saw what he missed. You don't need his custom plexi cover if you lay the box on it's side. The lid becomes a rear door, the side walls (now the top) are clear, you see right through them: I cut my arm holes into the bottom of the container (which is now the front of the glove box). It's a little topsy turvy I know, but if you look at a clear storage container on it's side, with the bottom being the front, you should get a clear visualization.
On either end of the box cut some circular holes and mount two power supply fans, just as shown in 9er Tek. Make sure the fans are thoroughly cleaned with disinfectant before mounting, then tape a pair of filter dust masks over each inlet. Two fans (9er uses one) provides enough positive pressure that you don't need the trash bag gloves he tapes over the arm holes. One fan is probably sufficient if you move in and out of the box slowly in a clean room.
My box is open: I put on a pair of cheap grocery store rubber gloves and wash my gloved hands and forearms in a few mils of rubbing alcohol. I enlarged the holes I cut into the bottom of the container and I can move in and out of the glovebox with ease, loading Jars, Syringes, etc. right through the arm holes. If I need to put in something larger I remove the back panel (lid).
Spray the inside of the clean loaded box down with Lysol until all surfaces are wet. Spray the dust filter masks that cover the fan inlets, turn on the fans, and let it sit until most of the Lysol has evaporated. Say 20-30 minutes. This gives the air a chance to exchange a few times, allowing the disinfectant time to work, and clearing out the dangerous (and toxic) fumes as it does so. Lysol fumes will kill the gills of a printing spore cap in just minutes.
This box is almost a hybrid between a true Sealed Glovebox and a Flow Hood... But it works very, very, well on the ultra cheap.
Be careful with flammable disinfectants and flame in a glovebox, they can (and do) explode! Sanitizer fumes are also highly toxic to mycelia. It all could get nasty
Gloveboxes : Shroom Glossary
Nanook of the North (Nanook)
|Posted on Friday, August 17, 2001 - 12:57 pm:||
Harvest 12V power supply fans from old computer equipment. Fire them up with a low voltage supply; I use a battery charger, computer power supply, old DC transformer... Whatever
Spend some quality time with Q-Tips and an alcohol dip. Wash the blades and housing two or three times with disinfectant. You can dip and swish briefly in fuel grade denatured alcohol. Mount the fan, filters, route and terminate your power leads... Let everything dry a few hours, connect your power supply, start working.
It works.... $15 dollars and 3.5 hours of time = killer glovebox
|Posted on Thursday, October 11, 2001 - 02:51 am:||
heres a glovebox hongus and i use... no positive pressure ... no contams in syringe making, spore printing, agar work, innoculations, etc. either. you could just make one with a big cardboard box and rubber gloves too... cardboard's low tek but it works fine whe you spray it down with clorox spray and let the air settle for a while... http://oscill8.homestead.com
Nanook of the North (Nanook)
|Posted on Wednesday, August 15, 2001 - 08:37 am:||
I really like positive pressure, it allows you to keep the front of the box open to the outside... Pretty damned good box for the time and effort: sturdy, clean, convenient, made from lightweight plastic. Most of all it is comfortable with very unrestricted movement.
I duct taped the lid to one of the long sides of the container and made a hinge... The lid, (now back wall of the box), swings up and open from the bottom. Contams are not dropping in there either. There is sufficient positive pressure with two computer power supply fans, one on either end of the box, to maintain pressure as I move in and out of the box. I wear a pair of playtex rubber gloves, I wash my clean gloved hands in a few mils of rubbing alcohol (beware of fire risk), and I can move my hands in and out of a clean box. This makes filling syringes a breeze, and I have yet to have a contam in my box.
Once you get a glove box and a Pressure Cooker you are into Cloning, Dextrose solutions, Syringes... All kinds of good fun stuff... But you need to assemble some decent equipment in order to do decent work. A glove box is mandatory for intermediate and advanced mycology, and your success rate will remain consistantly high if you use one.
9er did a good job working this box out. I am very happy with the changes I made to the original design.
|Posted on Thursday, October 18, 2001 - 09:59 pm:||
Well, I figured that the first question had a yes/no answer. And as for the second, I was thinking along the lines of oscill8's glovebox. I want to build one like your's Nan, but I've still got feelers out on salvaging some computer fans.
I do agree that the best way to follow a tek is to the letter (at least for the early going). That philosophy has worked well for me from the beginning.
(and I would never ask questions without reading and rereading the archived teks. even have hard copies, I'm no dummy)
Thanks for the advice.
|Posted on Thursday, October 18, 2001 - 11:17 pm:||
Hell if the fans are the only thing stopping you from building the 9er tek box, I would put the fans on hold until later... Just get a clear storage container and cut a rectangle piece of the plastic out of the bottom for your arms. Wear rubber gloves washed with a few mils of alcohol before putting your hands in.
For $10.00 and 30 minutes cutting the hole you can decide if it's worth $5.00 or $10 more and a couple more hours, adding a fan or two. Electronic surplus stores and computer repair places will sell you the fans cheap in 12V, used 120 volt fans are around if you keep you eyes out at surplus places.
Yeah for $15 you get a pretty deluxe box. For $10.00 your still doing pretty good. I would not hesitate to work in my box with the fans turned off, still much cleaner than without.
|Posted on Saturday, December 22, 2001 - 06:25 am:||
The only outlet on a positive pressure box should be the holes or opening where you stick your hands in.
As far a flow rates... That depends on the size of the arm openings... The bigger the opening, the more air you need to pump to ensure air is always moving outwards...
An open box will function well with only a slight positive pressure if the arm holes are moderate in size and you do not move quickly when you are withdrawing your arms.
Now a flow hood is another story, you need a HEPA and cage blower to sustain sufficient flow across the entire front of a hood.