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|Posted on Thursday, October 25, 2001 - 02:55 am:||
EZ Mycelium Sryinge: Birth & Shoot
>> I want to reiterate. I used the tek you posted where you scrape up, squirt water into, and suck up the mycelium from the bottom of the freshly-birthed jars. It's working very quickly, faster than anything else I'm doing, and no signs of contams yet. This is good, because it gives me another chance to save my B+, which is on its way out if I can't be mycologist enough to save it. <<
It's a good Tek. Birth a jar carefully (gloved hands?}, and ease the cake out sideways. We do not want the cake to drop out quickly. Hold the jar sideways and ease the cake out with clean or gloved fingers. Do not turn the jar upright, but tip it at a slight angle, squirt a syringe full of sterile water into the jar and stir with the needle tip. You want to thrash up some of the mycelium stuck to the glass and suck it back up into the syringe for inoculant.
You gotta birth anyway, and this Tek uses a minimum of sterile technique.
at albertsons(grocery store) they sell baster syringes (about59 cc's or 1/4 cup) 4 bucks larger gauge needle than normal, on the baster aisle
(Message edited by admin on January 11, 2004)