|Posted on Friday, October 26, 2001 - 04:23 am:||
Liquid Growth Medium
Huge thanks to The Walrus!
Mycelium can be cultured in a liquid (as opposed to a solid like vermiculite/brown rice flour) which you can then use to easily inoculate substrate and start mycelium growth much faster than if you were using spores.
You should first go to your local homebrew supply store and buy the lightest colored powdered malt extract (DME) they have. Mix 4% (by weight)[ed.note: this comes out to 1 tablespoon malt per cup of water] _dry_ malt extract powder with distilled water (boil it, and then mix in the powder. No need to let it cool to room temperature first). Pour this medium into any large jar with a metal lid, and make one small hole in the lid with a nail. Cover the top tightly with aluminum foil, and either boil the jar in water for an hour (be sure to put the jar on a washcloth and not directly on the bottom of the pot) or use a pressure cooker set at 15psi for 20 minutes. Let the jar cool overnight.
The next day take your spore syringe, stick the needle through the hole in the lid, and squeeze in the solution. Cover the top of the jar with aluminum foil again, only not too tightly since you'll want to allow gas exchange. Keep the jar somewhere warm and dark, and agitate it every day. You should notice the solution becoming milky, and towards the end of the incubation you'll see fluffy pieces of mycelium floating around in it. At this point you can keep it in the fridge until you're ready to use it. When you want to start a mushroom culture somewhere, flame-sterilize the needle of a 10cc syringe, let it cool, stick it through the hole in the top of the lid, and pull in as much solution as you need. You can then inject this into your substrate and it will start growing immediately. Any time you run low on mycelium solution, go through the same process with another jar only instead of injecting spores inject the last of your mycelium solution. Be on the lookout for any contamination!
Nan's Tips & Teks : Karo Tek : Liquid Culture Lids : Shroom Glossary
Tip: DME is hygroscopic (see desiccant), _dry_ weight is aprox 3.6 grams per level teaspoon... Nan
Neil Cooper (Phake_Ld)
|Posted on Tuesday, December 11, 2001 - 08:46 am:||
If my turtle is using a liquid substrate to grow mycelium (Malt Extract with distilled water), would he be able to suck the water itself into a spore syringe to innoculate a jar and promote colinization? or does there have to be chunks of mycelium in the spore syringe to promote colinization?
|Posted on Tuesday, December 11, 2001 - 11:56 pm:||
I wouldn't say chunks but the more strands of mycelium floating around the better. Stir it around gently with the needle and brake up the chunk, then suck it in.
|Posted on Wednesday, December 12, 2001 - 02:40 am:||
I thrash the liquid culture with the needle tip vigorously for a few seconds to fragment, then draw up the liquid into the syringe.
If it is too clumpy and the needle gets clogged, eject the liquid, plunk the needle back in and pull way back on the plunger and eject it back into the jar a couple of times... This usually breaks up all the clumps.