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Inoculation Discussion Tips & Teks - 1

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jared (Jared112)
Posted on Thursday, November 15, 2001 - 06:24 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

First take one of your jars that has been cooled down after steaming. I used white electrical tape on mine.
jar

Then you will want to lift the tape up on all 4 holes so that you can safely stick your needle in the holes.
tape up

The next thing that you have to do is flame the syringe for a few seconds in an alcohol flame until the tip turns red. I was using 91% rubbing alcohol, which left a tiny bit of black resin on the needle, but that's ok. I usually take a paper towel that has alcohol on it and wipe the needle down before flaming.
flaming

Next you will want to squirt a few drops of the solution out to cool down the needle, and to get all of the dead spores out that were killed during the flaming. This pic shows a lot of solution being squirted out, but don't worry it was just filled with water so I didn't have to waste spore solution.
squirt

Then you stick the needle into the substrate jar as far as it will go with the tip against the glass.
needle in substrate

Then squirt .25cc into each hole (1cc per jar) It is only a few drops, but that is all you need, there are hundreds or even thousands of spores in each drop. Be careful, because it's easy to squirt more solution in than needed thus wasting your solution.
spore solution

Then all you have to do is put the tape back over the holes, and wipe the needle down with the alcohol damp paper towel and put the cap back on and save it in a cool dark place until you need to use it again.

See: Clean Rooms : Incubation : Simple Cubie Tek : Pressure Cookers : Foil Covers : Syringes : Shroom Glossary : More About Inoculating
EDITORIAL NOTE: BE SURE TO USE AIR-PERMEABLE SURGICAL TAPE OR ELSE YOU MUST REMOVE TAPE BY 50% COLONIZATION.