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Inoculation - 1
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Sanitizing FAQ - 1
UV LightrelicImok Urok214 3 12-06-01  07:39 am

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Admin (Admin)
Posted on Thursday, August 23, 2001 - 12:19 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

GroverzGuide: How to make a cleanroom

Written by: GroverzNutz
email: [email protected]
ICQ: 82915143

Well, we all know that the most important part of success is having clean procedures. Well I've found that I can turn my bathroom into a sterile clean room both during innoculation and when removing any contaminants. By adhering to clean procedures, you should not have any problems with contamination. Here's my tips to making your own clean room:

Ingredients:

Bottle of Lysol antibacterial spray. it's sorta like Windex except it kills a huge shitload of germs.

Spray bottle with 10% bleach and 90% water mixed into it.
Wal mart has bottles for something like 49 cents.

Loads of paper towels

Dust mask for face, along with cap for hair and long sleeve shirt.

To prepare:

Close all airvents to the bathroom and draw curtain on your shower, wait about 10 minutes for dust to settle.

From here on out, any time that you are in the clean room make sure you are wearing long sleeve shirts, a hat on your head and a dust mask across your face.

Wipe down the area around the toilet with lysol, including the toilet itself, sink area, and floor underneath if you have tile.

After letting lysol sit for 30 seconds, wipe off with paper towels and lightly spritz with chlorine and water mixture.

Right before you open the jars, mist the air with the water/bleach mix and let it settle. Also do this anytime you enter the clean room with fresh jars or whatever.

If using a spore print, dip your instrument in rubbing alcohol and flame off before you use it to scrap spores. I also find this helpful for all instruments that touch the cake, including knives used to cut out any contamination, always flame off alcohol.

To store:

Once you've been real sterile innoculating the jars, put lid on real tight and follow whatever Tek procedure you chose.

I've found that even contaminated cakes will sometimes prodouce edible shrooms if you get right on top of the contamination and not let it get to the shrooms. Of course, any shrooms on a bad cake must be taken lightly in case they have mold, but its better than throwing it out.

First, separate the bad cake from the others. I usually put the cake on a tupperware container tall enough to let shrooms grow but narrow enough so that it doesn't take much perlite to fill the bottom. Put about a 1 inch layer of perlite, and damp with a mix of 50% water and 50% Hydrogen Peroxide into the bottom. Keep the lid on loose to allow air exchange but keep humidity.

If done right, this 'hospital' should be able to recover bad cakes that are edible. however i must stress that you should always start out very low with shrooms from a recovered cake. If they're ok you can work your way up just don't take a lot as there is an increased risk of bad shrooms.

[[ PURE CULTURE TECHNIQUE ]]

The most difficult part of psilocybin mushroom cultivation is the observance of the rules of pure culture technique. These are the sanitary code of mushroom cultivation. There are usually many varieties of bacteria and fungal spores in our environment; floating in the air, clinging to our hands and clothing, issuing from our mouths with every exhalation. Extreme measures must therefore be taken to keep these out of our mycelial cultures, which they would rapidly overrun. The following points should be diligently observed. Work in a clean, uncluttered, dust free room. Immediately before work wash the work table and spray the room with disinfectants. Scrub arms, hands, and nails with disinfectant soap. Wear simple clothing. A freshly cleaned short-sleeve T-shirt is ideal. Gargle with antiseptic mouthwash and cover the mouth and nose with a clean cloth or disposable surgery mask. Cover the hair with a surgical cap or shower cap. Allow no drafts in the room. close all doors and stuff all door jambs. Let no flies, animals, or unnecessary people in the room. Let only sterilized equipment touch the medium or inoculum. Don't lean over your work. Avoid all swift movements that may cause a draft. If possible have a hood constructed around the work table or a screen or curtain surrounding it. Be neat and keep all materials within reach. Keep all equipment about three feet away from the work. Do not permit anyone to enter the room while work is in progress. ]]

Related Link: Sanitizing
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Ink drop (Headrastaman)
Posted on Wednesday, November 07, 2001 - 02:37 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

i'm gonna try growing in my closet... should i be worried about molds or anything growing in my closet as a result of growing mushrooms.... thought i'd just check first.. thanx
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Nan (Nanook)
Posted on Wednesday, November 07, 2001 - 02:58 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

If there is a problem with Sanitation you are doing something wrong. You want to turn your closet into a mini-"Clean Room". Pitch any contamed cultures as soon as they are discovered. Be ruthless.
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jared (Jared112)
Posted on Wednesday, November 07, 2001 - 03:48 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

When I preped my closet I wiped down all the walls with bleach and water solution about 10%bleach 90%water. Then I vacumed the carped and after I wiped down every square inch of that closet I put the solution into a spray bottle and sprayed the ceiling and the walls and the floor everything... I did that over a week ago and it still smells like a sterile laboritory inside.
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Lichen (Lichen)
Posted on Wednesday, November 07, 2001 - 04:13 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

sounds good...just keep the humidity down in there. If you have high humidity in the closet itself, you will grow mildew....saw a nice crop of it in someone's pic recently...ruined the whole room
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Nan (Nanook)
Posted on Wednesday, November 07, 2001 - 06:58 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I have found that if you have carpeting and plaster to deal with, tape down some plastic sheet to the floor and walls: it cuts down on both Trich and Cobweb, protects the carpet and plaster from water and Verm Particles, and helps keep the Temperature and Humidity up

Shroom Glossary
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plinkerdink420 (Plinkerdink420)
Posted on Friday, December 21, 2001 - 05:26 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

let's say that someone wanted to convert a linen closet into a terrarium of sorts.... if they were to line the walls and floor with a thin plastic tarp and place a cool mist humidifier in there... would there be any potential problems.... also if this person were to do this, they would install fans in the walls of the closet to ventilate... will the moisture somehow bead through the plastic making the wood rot?...
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Imok Urok2 (Imok)
Posted on Friday, December 21, 2001 - 07:25 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

If you put on 4 or 5 coats of polyurethane
(the kind they use on ships)
you wouldn't have to worry about
putting down plastic or
the wood roting
(caulk first)
You could have up to 100% humidity
with no problems that way :)
You could even install a attic vent
bathroom fan for total stealth.
Just be sure to put down a lip
at the door to keep any moisture
in the room and from leaking out
under the door.
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plinkerdink420 (Plinkerdink420)
Posted on Friday, December 21, 2001 - 01:49 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

thank you very much for the thorough response... i thought about the paint thing... i just thought that the plastic would be better, but never actually tried the paint... i just figured if it was easier, then, with my luck... it wouldn't work

* Plink, you can use plastic as well just fine. Use good quality duct tape to seal things up. Make sure the floor is covered, and the plastic on the floor should be a couple of layers thick, and tape it to the wall at some point above the molding. This will contain water spills and leakage, and seal out contams. Clean everything well _before_ cutting and taping plastic.