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Honey Tek - 1
Contams in Liquid Culture - 1

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Mr. Tambourine Man (Tambourine_Man)
Posted on Wednesday, December 12, 2001 - 04:17 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Hi, a FOAF has a honey jar about 3 weeks old that has a HUGE MASS of white/yellow "stuff" in it. He's not sure if it's mycellium or some kind of contaminant. He has pictures but no place to have them hosted. The mass of mycellium was attached to the bottom of the jar but has since detached and the area that was attached is a bronze color. There is also some of the same material growing from the surface of the water down into the liquid in much smaller quantity. What do you guys think?
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Imok Urok2 (Imok)
Posted on Wednesday, December 12, 2001 - 04:35 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Attach your pics to your post. (check formating on the sidebar)
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plinkerdink420 (Plinkerdink420)
Posted on Wednesday, December 12, 2001 - 04:35 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

doesn't sound good man... never saw any stuff like that......
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Nan (Nanook)
Posted on Wednesday, December 12, 2001 - 05:04 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

If it were me I would pitch it. Your alternative is to test a single PF jar... But I don't open anything funky in my Glovebox.

Liquid Teks are cheap, costing pennies per jar (if that) in materials and energy... Unless you shoot commerical Spores, then your cost rises to a buck or two.

For this reason I am very selective about which cultures I will load into syringes and use to Inoculate jars. I make up a several liquid culture jars and only use the best ones.

I am a big fan of clear liquid culture tek because after running 2-3 jars in crystal clear liquid media your eye becomes trained and will tell conclusively which jars are running hot and true. It is much easier to spot carmelization...

I am thinking about shopping around for some smooth wall jars to make it even easier to see inside
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Mr. Tambourine Man (Tambourine_Man)
Posted on Wednesday, December 12, 2001 - 05:09 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

c: c:mush1.jpgmush1.jpg
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Nan (Nanook)
Posted on Wednesday, December 12, 2001 - 05:12 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Nasty Dude!
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Mr. Tambourine Man (Tambourine_Man)
Posted on Wednesday, December 12, 2001 - 05:13 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

The above pic is of the mass rolled onto its side in the honey water. The dark area is where it was attached.
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Mr. Tambourine Man (Tambourine_Man)
Posted on Wednesday, December 12, 2001 - 05:23 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

2
3
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Nan (Nanook)
Posted on Wednesday, December 12, 2001 - 05:29 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

If you are still in doubt, thrash the mass with a needle, draw up a cc or two and shoot a jar.
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hippie3 (Hippie)
Posted on Wednesday, December 12, 2001 - 02:08 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

looks like a mycellia-filled jar of honey water to me. do as nan suggests, test it on a few jars.
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property of Friedrich Nietzsche (Economist)
Posted on Wednesday, December 12, 2001 - 08:21 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

can you just pop a piece of shroom/mycelium tissue in a PC'd jar of karo and water under sterile conditions and have it grow? or do you need to do something to it first?
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property of Friedrich Nietzsche (Economist)
Posted on Wednesday, December 12, 2001 - 08:25 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

and how much mycelium do you need in a jar? I followed the above procedure, and I have 1/2 inch balls of fuzz growing off the pieces of mycelium I popped in a little over a week ago. is this good?
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Nan (Nanook)
Posted on Wednesday, December 12, 2001 - 11:46 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

You are describing cloning. Yes you can clone as you describe. There are sterile procedures that should be observed in obtaining a piece of sterile mushroom tissue, and it should be macerated for the best results. It does not take much to get a clone jar started, just a few clumps of sterile cells swirled around in there for a few days.

Sounds like your is doing what it is supposed to, though like I said it really helps to macerate the tissue somehow.

Read about the Cloning Teks
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Mr. Tambourine Man (Tambourine_Man)
Posted on Thursday, December 13, 2001 - 06:52 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

1

Notice the things floating on the surface, they kind of appear to have bluish dots of them too. Anyone ever see mycelium grow from the surface like this? I've seen a lot of contams in honey jars, with blue and green all over and this isn't the same thing. The drapery from the surface spots LOOKS like mycelium. What do you think?
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Nan (Nanook)
Posted on Thursday, December 13, 2001 - 07:04 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I have yet to have floating pads of mycelium in liquid culture unless it was contammed.
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Canadian Mushroom Grower (Canadian)
Posted on Thursday, December 13, 2001 - 03:09 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Could someone post a pic of a good honey/water/mycelium jar that is ready for innoculating or syringes? Thanks.
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Canadian Mushroom Grower (Canadian)
Posted on Thursday, December 13, 2001 - 03:19 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Oh yeah, and one day after taking some syringes of mycelium, I decided to smell the solution(since I no longer had a need for it) to check for any unseen contaminates. To my surprise the solution smelled acidic kind of like vinegar, and the honey water stuff looked OK to me. Has anyone else noticed this?
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hippie3 (Hippie)
Posted on Thursday, December 13, 2001 - 04:54 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

a vinegar smell is not good. hopefully it contam'd after you drew your syringes.
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Mr. B (Argonaut)
Posted on Thursday, December 13, 2001 - 10:43 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

trash that orange one dude. Can't you hear that one barkin' from inside the closet at night??

Now them two fat ones white things look interesting alrighty. If that's what is looks like, it's gotta be a record fuzzball!!!