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Are spawn jar air exchanges required?Ryan WatersRyan Waters3 1 12-20-01  07:27 pm

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mushmasterflex (Mushi)
Posted on Tuesday, November 27, 2001 - 06:39 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

This is my first shot at growing shrooms and I followed the pf tek and used his pf spores. I steam sterilized the jars and inoculated them after cooling down. Its been over 5 days and I see no growth at all. Also, the jars were misplaced on a heating pad overnight and were warm to the touch and removed from the pad. I'd say they reached 90F but had no thermometer to be sure. How much heat can the spores survive thru? I'm not sure where I could have gone wrong in following PF tek except the heating pad incident. I dont see any discoloration or smell of the substrate so I think the jars are still sterile. I really dont know where to go from here... just throw a little knowledge this way please I'd really appreciate it.
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Tripster (Tripster)
Posted on Tuesday, November 27, 2001 - 07:09 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I'd say that you should wait a few more days to see if anything starts to grow, before you do anything else. What temp do you keep the jars at regularly? And how long do you think they were at the supposed 90F? did you pack the substrate down at all? There could be a lot of reasons as to why you haven't seen anything yet. Sounds to me like you should be ok as long as you followed the tek, however this is just my humble opinion. Good Luck.
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mushmasterflex (Mushi)
Posted on Tuesday, November 27, 2001 - 07:17 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

The jars sat for about 6 hours or so at that temp. The ambient temp around here has been on the chilly side, 60-68F. I keep a blanket over the jars to keep them a little warmer. I did pack the substrate down and filled the remaining space with verm. I know I should give it some more time but I am worried that I killed the spores. Thanks for the input Tripster.
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ggg (Ggg)
Posted on Tuesday, November 27, 2001 - 09:33 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

jars

cakes

I think your dead at 106
while incubating try to stay warm not hot!
Keep it clean-
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hippie3 (Hippie)
Posted on Tuesday, November 27, 2001 - 10:30 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

that's too cold.
if you want those spores to germinate,
you'll need to get that temp into the low 80's and hold it there 5-7 days.
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wycrobro (Newman)
Posted on Tuesday, November 27, 2001 - 03:48 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

From what i have read they wont germinate at all at that temp. Try an incubator there are good cheap teks in the arc.
Or try putting the jars in some sort of container and put it on the heating pad. Start low then turn it up as needed? I think this should work.
good luck
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Snoopy (Snoopy)
Posted on Tuesday, November 27, 2001 - 07:37 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Also....... wrapping a blanket around them won't help inless you have a heat source within.. THe blanket will retain heat, but if there is no heat in there in the first place than it isn't doing much good other than keeping the light out...
Food for thought
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hippie3 (Hippie)
Posted on Tuesday, November 27, 2001 - 07:56 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

interestingly enough, the jars do generate some heat of their own [once germinated].
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mushmasterflex (Mushi)
Posted on Tuesday, November 27, 2001 - 08:32 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Thanks for the advice guys. I just got a styrofoam box which I think will work well as long as I get a heat source inside. If anyone could suggest where I might be able to get a thermometer, please do. The local supermarkets and drug stores don't seem to have any.

As for the blanket idea... It was just plain thinking that blankets keep me warm at night. Stupid me.
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mushmasterflex (Mushi)
Posted on Wednesday, November 28, 2001 - 12:05 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Okay, I think I got the incubation problem covered. The styrofoam box plus a set of 100 white christmas lights seems to be a good mix. I still dont have a thermometer to be sure but its warmer than the ambient temp. If anyone sees any faults with my makeshift incubator please do let me know. Also, if any other information is available on how much heat spores can take, please post. I know there is only one way to find out if my spores died but if any other info is out there, I'd be more than happy to know.
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Lichen (Lichen)
Posted on Wednesday, November 28, 2001 - 12:08 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

well, a simple thermometer can cost you as little as $3. Get one. It's the only way to make sure your incubator is right. The problem with incubators is the ambient temp outside the box can affect the temp in your box.

Spores should not be stored any higher than 75F
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jim brown (Shrhobbyist)
Posted on Wednesday, November 28, 2001 - 09:02 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

If you can't find a thermometer you can't be looking too hard. Do you have any '-mart' stores near you?

By any chance is Ggg Greenthumb? If so, why the new name?
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ggg (Ggg)
Posted on Wednesday, November 28, 2001 - 09:24 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

sick of typing that name out.
I used to be able to grow things-
Won't be able to prove that again until spring!!

greenlessthumb
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jim brown (Shrhobbyist)
Posted on Wednesday, November 28, 2001 - 09:34 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

What will you be growing come spring, if you don't mind me asking? And what do you mean by "used to be able to grow?" Is it a problem of location or did you lose the touch. Oh well, welcome to mycotopia Ggg. Thanks for all the links!
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ggg (Ggg)
Posted on Wednesday, November 28, 2001 - 10:32 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Full blown 1/2 acre veggie garden.
Hot peppers out the ass-
I make my own hot sauce and can my veggies so I hate winter. I can't grow shrooms cause my designated grow spot is too dirty.

Thanks for the warm welcome Jim
CU soon
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jim brown (Shrhobbyist)
Posted on Wednesday, November 28, 2001 - 10:50 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Me and my girlfriend grew oregano, parsley, chives, basil, tomatoes, beans and garlic this summer. Enough spices for about 3 years of heavy use. We dried the spices and that made them suck! We froze the rest and that keeps them near perfect. But, damn, we lost a lot, already done with the parsley, which we both love. Oh well, we had fun doing it and will have fun doing it again next summer. My folks have had there gardens featured in a few national magazines. They built a greenhouse 2 years ago to keep stuff going all year long. Man, I could use that greenhouse!
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wycrobro (Newman)
Posted on Wednesday, November 28, 2001 - 03:35 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Well mushmaster. the lights in the incubator might be a problem?
I would suggest the styrofoam tek in the arks Im not sure of the link but im sure someone can post it for you.
As for the thermometer I use one of those floating fish tank ones, I'v cut a hole just big enough(make it small and force it through) the thermometer is round and goes half way in so you can read the temp with out opening the incubator.
GOOD LUCK
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mushmasterflex (Mushi)
Posted on Wednesday, November 28, 2001 - 04:36 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Alright a have a mini update. I got me a cheapo thermometer after a long search and 100 christmas lights are a little too hot for the mycelium. The temp reached about 100F and now I am @ 50 lights @ ~80F. I will continue to tweak it til it reaches about 85F, sounds good? And another possibly good note, I see some white growth in one of the jars in one of the innoculum sites. I hope they pull thru.
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hippie3 (Hippie)
Posted on Wednesday, November 28, 2001 - 04:48 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

sounds like you're on your way...
trip
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wycrobro (Newman)
Posted on Wednesday, November 28, 2001 - 05:42 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

What about the light? unless he is using your neg tek or going for invetro pinning wont this be a problem hippie?
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mushmasterflex (Mushi)
Posted on Wednesday, November 28, 2001 - 05:59 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Wycrobro,

I'm not sure what I might do as to after if the cakes colonize. Invitiro sounds good but a bigger yield for christmas shrooms sounds muy bueno. I'm still contemplating that... or maybe I'm counting eggs before they hatch. As far as my knowledge goes, light won't hurt the mycelium while its colonizing, so I *think* I'm safe.

Hippie,

That is one trippy picture. Might you happen to have one in a bigger format so I could put it on my desktop?

Thanks to everybody for their assistance and I will keep you guys posted.
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Ron (Clubbenguy)
Posted on Wednesday, November 28, 2001 - 07:06 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Good luck, I go to retile shops for my thermometer/humidity gauges. They come in a two pack you can just stick to the inside of the walls of your grow chamber/incubator.
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mushmasterflex (Mushi)
Posted on Wednesday, December 05, 2001 - 11:54 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Hello again, I have a little update for those who might care and another small question. My styrofoam incubator is doing the job but it is doing it rather oddly. There is enough space in the container to fit 8 jars on the bottom and plenty more room vertically. So i stacked the jars 8, 8, 4 which gives a total of 20. The thing is that the bottom 8 have colonized much faster than the other 12. I'm not freaking out or anything but just damned curious. I'd say the bottoms jars are about 70% and the rest are barely starting out @ 20-30%. I got the temp in there @ 86F which seems optimal. The source of the lighting are christmas lights and I have them running in between jars and evenly distributed. I was thinking that heat rises and it would be cooler at the bottom of the incubator. Would that mean I should be lowering the temp? Eh... I'm confused.

Above all, I'm happy my spores didn't die and hopefully I can look forward to X-mas shroomies.
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SYDYSTYK (Addict)
Posted on Wednesday, December 05, 2001 - 11:57 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

rotate the jars then-top to bottom
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hippie3 (Hippie)
Posted on Thursday, December 06, 2001 - 01:12 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

odd.
but it's best not to wonder about somethings too much.
moving the jars around sounds like a good option.
btw, i don't have that pic sized,
you should be able to set it as wallpaper, just right-click on it,i think, but i'm no 'puter xpert.
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mushmasterflex (Mushi)
Posted on Thursday, December 06, 2001 - 10:24 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Another quick question... The bottom jars are reaching full colonization. Should I take the tape off the innoculum holes? Let the mycellia breathe and promote growth? Thanks in advance.
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Nan (Nanook)
Posted on Thursday, December 06, 2001 - 11:28 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

You can remove the tape. Some people flip the jars. If the jars are going good it's personal preferance.
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gnas (Psystep)
Posted on Thursday, February 07, 2002 - 07:51 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Hey guys, if your growing invitro or have a set-up inside your room, which includes a computer, one way to raise the temperature is to always leave your computer moniter on. (with a screensaver ofcourse so you don't burn the screen up)
I found this the best way to warm up my room since I don't have temperature control here and no incubator.
Better yet, set your jars on top of the monitor with some kinda barrier.
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Chronic007 (Chronic007)
Posted on Thursday, February 07, 2002 - 08:06 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

geesh, your monitor puts off that much heat? Dos the rest of the computer take up the entire room? Just playing!
Chronic007