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Nan's Cloning Tips & Teks - 1

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Jose Cruz (Vato)
Posted on Saturday, December 29, 2001 - 09:18 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

I have been reading alot of info lately, and wondering how people come across clones of monsters. Do most people clone big'ns from own flushes and then grow out liquid cultures for inoc. How do you know what to clone or if its worth the effort..... I know qite alot about being a greenthumb, and its cloning process. However I have many things to learn about being a
whitethumb.....Any takers?
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Nan (Nanook)
Posted on Saturday, December 29, 2001 - 10:10 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

You grow some shrooms from spores, then you keep your eye's open for an outstanding shroom. I like to pick a very large shroom from a cluster... While your waiting for your special shroom to pop up... You get your Tek ready to run... Glovebox, sterilize some liquid culture jars, get your blender and/or blades ready... Then you clone.

When you clone into liquid, you simply thrash or macerate the tissue, draw it up, and shoot jars.
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Brad (Raze)
Posted on Sunday, December 30, 2001 - 01:13 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Could you simply peel the outside of a mushroom off (to get to the sterile inside) then take a cross section it and drop it in a jar over an oven rack? I tried to clone using H2O2 but it never germinated. I think I let it sizzle too long, or the substrate was bad (used my own ground rice rather than preground stuff)
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onediadem (Onediadem)
Posted on Sunday, December 30, 2001 - 02:15 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Good question Brad. Is a blender necessary? could one just mince the peice up with sterile razor and drop that into the sterilized liquid?
pj
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Cragith Kilbonith (Kilborn)
Posted on Sunday, December 30, 2001 - 02:40 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

From what I heard you can just chop it up into small pieces then put it your karo/dextrose jars. Like I always say correct me if im wrong.
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Fishy1 (Fishy1)
Posted on Sunday, December 30, 2001 - 03:50 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Yes, that can work. Did it with Pan Cyan, and let it grow out like a multispore liquid jar....it worked great in 2 or 3 jars.
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Nan (Nanook)
Posted on Sunday, December 30, 2001 - 07:56 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Yeah a lot of this is detailed in my Tips & Teks... And you can combine/mix teks a bit to meet your situation.

You can peel the surface tissue from any section of clean fresh mushroom, remove a sample from under the surface, place it on a sterile plate, and macerate it into pulp with a sterile blade (exacto works beautifully). Scoop up a tiny blob (a match head sized lump is more than enough) and drop it into a liquid culture jar. The jars are ready to shoot in 3-5 days.

Tip: A good way to transfer macerated tissue off of a plate and into jars is to use a sterile eye dropper as part of your tek... Drip a drop or two of water on the shroom tissue, macerate with a blade, add more water if needed, then mix with the dropper tip while sucking the watery tissue up. Use one drop per 100 cc of liquid culture tek.
This works great in the depression of a sterile ceramic saucer.

You don't need a blender, you do have to be exceedingly sterile.

Avoid the use of H2O2, the only time H2O2 is required is to clean up a much needed contamed culture, or to cover sloppy Tek... H2O2 liquid solution quickly kills mycelia, especially macerated mycelia, and so must be used with caution.
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Mr. Tambourine Man (Tambourine_Man)
Posted on Thursday, January 03, 2002 - 07:30 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Would it work to clone with a small chunk of a shroom without macerating it in a blender first?
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Hatcher (Hatcher)
Posted on Thursday, January 03, 2002 - 09:27 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Clone dry mushroom Nan? Very interesting...
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Nan (Nanook)
Posted on Thursday, January 03, 2002 - 10:13 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Fixed... Thanks for pointing that out
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plinkerdink420 (Plinkerdink420)
Posted on Thursday, January 03, 2002 - 01:53 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

H2O2 Cloning Tek i think is what you are looking for Mr. Tambourine Man


i think i am understanding your question differently now... someone suggested slicing it up with a razor blade as an alternative
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hippie3 (Hippie)
Posted on Thursday, January 03, 2002 - 04:37 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

it definitly needs to be cut up finely, else it'll just sit there forever.