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Mysterious Stranger (Supersonic_slug)
Junior Member
Username: Supersonic_slug

Post Number: 19
Registered: 01-2004
Posted on Tuesday, January 13, 2004 - 05:30 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Hi all!

Starting up my invitro grow project, and i have a few questions.

1) What sort of timeline am I looking at? Yes, i know, that's very dependent on growing conditions, etc, but how about a typical, ballpark estimate? How long to colonize (a month or so, I'm figuring), how long for first flush, and how long between flushes?

2) How important is humidity? The tape is off my injection holes from day one (this is correct, right?) so I assume some air exchange is taking place. I live in a northern clime with central heating, so indoor humidity is very low at this time of year. Are my cakes likely to dry out? Should I put some form of humidification in the closet my jars are stored in?

3) Anyone ever tried hip's neglect tek with tyvek on the jar lids? Would the advantage of increased air circulation be counter-acted by problems with the cake drying out?

4) my thoughts are that Hippie3's neglect tek is probably the single easiest tek for beginners. My plan is to get a whack of invitro jars going, and then start trying out casing, terrariums, etc. That way, even if (when) I screw up, I'll probably still have a backup supply of mushrooms from invitro to keep me from getting too discouraged. Thoughts?

(Message edited by supersonic_slug on January 13, 2004)
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Soliver (Soliver)
Senior Member
Username: Soliver

Post Number: 515
Registered: 03-2003
Posted on Tuesday, January 13, 2004 - 09:40 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

1) Hard call - you've done your homework so you
know the standard answer... anywhere from 3.5 weeks to
two months IME

2) I'd get some tape over those holes - masking tape
is my personal favourite, but breatheable bandaging
is OK too (even if the adhesive does make a mess)
Otherwise, humidity isn't a big issue. The whole
idea is to have a minimalistic setup, so no humidification is
required or needed.

3) A post I made last week on a similar thread:

I prefer invitro, as it suits my lifestyle best.
In my fridge I have 12 or so invitro jars that were
full of shrooms back in August '03.
The shrooms are a deep blue color and VERY potent
after sitting in the jar so long - I don't know
why, but I'd guess 2x as strong as "normal" shrooms.

If I need a larger flush for an upcoming occasion,
I pull an invitro jar from the fridge, pick off the
blue shrooms (and save for my personal use)
then I dunk the cake and fruit it in a pint jar
or small terrarium.

This way, I can mothball my hobby and resurrect it
whenever I want without having to go through the
hassle of innoculating, waiting, waiting, waiting...

When I DO innoculate jars, I'll do 30-40 of them
at a time. I keep them in a warm spot until the
fruits are as big as they'll get (1st flush) which
is more than you'd expect...

Usually the fruits will start to split and bruise
a bit - that's when they go into the fridge. I have
2 refrigerators, so I have an entire drawer in the
crisper section full of 1/2 pint jars laid on their
sides for space considerations.

I jam the jars into the drawer in such a way that the
casual observer is unable to open the "jammed" drawer -
sometimes the best way to hide things is
to not hide them at all.

One weekend my Mom was visiting. She did me a "favour"
and got the stuck drawer open. I told
her the jars were part of my upcoming / ongoing
worm farm that I had mothballed for the winter season
and that the worms laid in stasis this way.
People believe what they want to, and besides -
only a shroomer would EVER guess what was really in
those jars ;)

FYI- once an invitro cake is fruited in a larger jar,
they don't like to be returned to invitro stasis or
growing invitro. Mine contam at least 90% of the time if I try
to return them to a closed growing environment.
Dunno why - once they get to stretch their legs out,
they don't seem to like going back to the box...

hope this is of use to those contemplating invitro
growing. For the small-scale, personal use grow,
especially if you're a "professional" (i.e. work more than you play)
it's an ideal way to stay sane while cranking the
handles of the machine...

BTW - I always keep some invitro jars going, no
matter what else I'm messing with. It's $ in the
bank (well, in karmic dollars, that is)


Soliver
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Nilla (Nilla)
Senior Member
Username: Nilla

Post Number: 531
Registered: 05-2003
Posted on Tuesday, January 13, 2004 - 10:00 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Thats a great idea Soliver.When you put the fruited jars in the fridge how long can you leave the fruits on till you decide to pick?
And after picking/dunking,how are the remaining flushes like?
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Soliver (Soliver)
Senior Member
Username: Soliver

Post Number: 518
Registered: 03-2003
Posted on Tuesday, January 13, 2004 - 11:08 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

So far I'm up to six months -
These are PR and HAW - I know the PR's aren't
"supposed" to grow invitro, but mine sure as hell
do! I fruited a cake three weeks ago after a 48 hour
dunk with satisfactory results. I've never had
shrooms grow from cakes like some pics you see here,
(phat's old setups, etc.), but it's enough for me.
I got a nice 2nd flush out of the cake for a friend's
b-day party, dunked again and put it back into the
terrarium but then it contam'd... more due to my
inability to take care of it than anything, IMO.
Sometimes my cakes go up to two days with no
maintenance, so you can imagine the disatrous averages
I have with 3rd and 4th flushes...

Like I said, I'm in this for personal use only -
it's easier for me to knock up 30 invitro jars and
get 2 flushes from them than to maintain a multi-
flush setup, from a time perspective, and I'm still
floating in shrooms...

The only thing I'm fastidious about is spore printing -
I'll keep one or two cakes from each new strain in
super-clean isolation (well, for me at least) until I get fifteen + good
prints and have them stashed for safe keeping.
Have print, will travel - that's my motto!

Soliver
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Soliver (Soliver)
Senior Member
Username: Soliver

Post Number: 519
Registered: 03-2003
Posted on Tuesday, January 13, 2004 - 11:36 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

OK - looks like I lied. These aren't from August.
I moved in August. These were innoculated on May 5 of 2003.
These are both PR clones I was messing with at the
time - note that the fruits are dessicated, but if
you were sitting here with me all you could smell
would be the sweet smell of an invitro cubie crop.

I'd guess the first invitro flush here to be a few
grams dry - nothing to write home about, but then
again, they're eight months old and still fresh!
(crop in last pic, on the plate, left side of pic)
If I had picked 'em when freshest, they would look
larger, but dessication occurs (shit happens)...
the shrooms are going from grey to blue as I type..

I'm dunking this cake as we speak - I'll toss it
in a plastic shoebox terrarium and post results as things pop up.

A few pics (note the date tag on the foil):

Upload
Upload
Upload
Upload

Invitro rocks.
Keep in mind I 'nocd these jars almost a year ago,
and I just cracked one now for shits and giggles...
show me a casing that has such ease of maintenance
and consistent performance for the home-doser!

:-)

Soliver

(Message edited by soliver on January 13, 2004)
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 10723
Registered: 02-2001
Posted on Tuesday, January 13, 2004 - 11:50 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

great details there.
archive material
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Nilla (Nilla)
Senior Member
Username: Nilla

Post Number: 537
Registered: 05-2003
Posted on Wednesday, January 14, 2004 - 12:20 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Yes, very cool stuff thanx for sharing:-)
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Soliver (Soliver)
Senior Member
Username: Soliver

Post Number: 520
Registered: 03-2003
Posted on Wednesday, January 14, 2004 - 01:38 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)



My cup runneth over!

:-)

Thanks,

Sol
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big slick (Baddaboom)
Senior Member
Username: Baddaboom

Post Number: 111
Registered: 10-2002
Posted on Wednesday, January 14, 2004 - 12:16 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

If I am to understand you correctly Soliver, you believe your invitro shrooms increase in potency while being stored in the fridge.
Everything I write is a total work of fiction

Never give up never surrender
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Imok Urok2 (Imok)
Moderator
Username: Imok

Post Number: 739
Registered: 07-2002
Posted on Wednesday, January 14, 2004 - 10:21 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

That very effect of cold storage has been reported
anecdotally before here.
This would be an ideal way to provide a controlled
environment for this type of experiment.
Although, unless you have the needed lab equipment,
the results would only be subjective instead of objective.
Hope this helps :-)
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Soliver (Soliver)
Senior Member
Username: Soliver

Post Number: 522
Registered: 03-2003
Posted on Thursday, January 15, 2004 - 11:31 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

"If I am to understand you correctly Soliver..."

Yep, that's my take on it - of course, as IMOK
suggests, there's no way for me to actually test
this, but I've actually been scared from eating
1gram of dried invitro fridge-fruit. Trips come
on strong and hard, more like eating 2-3 grams IME.

The wife won't touch 'em for the same reason -
dosage is difficut in fractions of a gram (no digis here)
so she sticks to the "normal" variety.

:-)

Soliver
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Babylon Jasmine (Pergamos)
Intermediate Member
Username: Pergamos

Post Number: 53
Registered: 12-2003
Posted on Saturday, January 17, 2004 - 05:28 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

ok, after doing a cake invitro it can be transferred to chronic or a terrarium, how about regrowing a second invitro flush? should the cake be dunked? is it a bad idea over all? any comments?
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Quark (Quark)
Member
Username: Quark

Post Number: 23
Registered: 10-2003
Posted on Saturday, January 17, 2004 - 05:39 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

it should be fine for a second flush. you probably want to let the mycellum repair itself if you broke it up to get the shrooms out, and then just fill the jar the rest of the way up with water, put it in the fridge 12-48 hours, and you'll be all ready for a second flush, probably better than the first.
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Smerd (Smerd)
Senior Member
Username: Smerd

Post Number: 334
Registered: 05-2003
Posted on Saturday, January 17, 2004 - 06:29 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Sure. Dunk it and go again. You can go for three or even four dunks. After four, the cake gets sad-looking and mushy (the bad mushy, not the good mushie).
Initiative comes to those who wait.
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Hippie3 (Admin)
Board Administrator
Username: Admin

Post Number: 11043
Registered: 02-2001
Posted on Sunday, January 18, 2004 - 03:27 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Babylon Jasmine ,
pick it clean,
dunk it 24 hours
then return to fruiting conditions for next flush.
4 flushes is pretty easy.

(Message edited by admin on January 18, 2004)

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