The results are in. As far as metabolic fluid killing bacteria its negative. I even did a wet mount using metabolic fluid collected from reishi with no fatalities to living bacillus. I did take pictures to make this experiment more visual.
Check out all of the metabolites that have collected in the bottom of this bag.
Has anybody successfully used this to clean up or fight contams on agar plates or used in tubs before?
I read about this being done before I just wanted to know if anyone here has tried this.
Jar of grain spawn that metabolic fluid was collected from
Plate of cultured bacillus
Gram stain of bacteria showing pink rod shaped Gram negative bacillus
Different enzymes have different metabolic functions. If a culture is exposed to something repeatedly, it will develop a enzyme that will either break down and consume as energy or control or eliminate. Most fruit bearing fungi already pocess natural bactericides but as the bacteria evolves so must the fungi and what may take millions of years for organisms such as ourselves, fungi can evolve over a single generation or even as quickly as it can colonize a agar plate. Fungi evolve at the blink of a eye relatively speaking.
I speculated that it was eliminated from my tubs and maybe it hadnt been, mayne it still exists. As lactobacillus is considered a competitor for carbohydrates and in fact i believe it would be considered a hog, at some point i would think the mycelium would want to eliminate it from the substrate.
On the contrary if the mycelium colony is spending significantly less amount of energy on cellular respiration because of the lactobacillus is doing half the work, maybe the mycelium will continue this symbiotic relationship.
Forget all that, but if you repeatedly exposed your culture to that culture of bacteria, it will become resistant to it and or develop a emzyme to control or destroy it. Maybe destroy is inaccurate and i should say disrupt it, temporarily weakening it long enough to take the substrate isolating the bacteria colonies where they are limiting its food source and or its ability to take anymore market share of the substrate lol
So the metabolic fluid was extracted from the grain jar. IMO it is possible that the mycelium did not spend energy creating the enzyme to fight off the bacteria. Mycelium is very in tune with its enviroment while chemical signals send information throughout the entire colony. If no where does any part of the colony come in contact with a competitor organism, it probably wont flip that switch to produce the enzyme.
I think a good experiment would be to take metabolic fluid from a contaminated grain jar and then a clean grain jar. I believe that they will have many of the same enzymes but the contaminated one will have 1 or more enzyme not present in the clean jar.
How could we as citizen scientists comduct this test?
To sum all this babble up,
Primary and secondary metabolites can be influenced by the enviroment from carbon and nitrogen sources, temperature, ph, other organisms, and etc we cannot conclusively say that metabolic fluid secreted by mycelium does not destroy bacteria. Perhaps the metabolite you harvested was produced simply for digesting the grain.
Regardless great experiment and we need more of this around here. This info and info alike may not be practical to everyone as we all have our own expectations and goals from growing for medicinal value, growing for profit (edibles), some are here for the social networking with Awesome People , and so on but for information alone is why we need to see this.
Edited by Microbe, 02 February 2017 - 01:44 PM.