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Catattack some Agar 3.0


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#861 CatsAndBats

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Posted 25 November 2019 - 07:58 PM

This guy is about to push the lid off....


What should my nute be for my next round of transfers? I've done LME and BRF. Looking for something spicyyyy, I know potatoe flakes are kind of vanilla but I may try that next.

Also I have active yeast, I'm pretty sure that was in your first post. You cook it before hand right? but wouldn't thePC kill it and turn it into nutritional yeast?

 

Potato flakes are more beginner friendly than yeast.

 

Is that thing airtight?



#862 macgyver

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Posted 25 November 2019 - 08:05 PM

 

This guy is about to push the lid off....


What should my nute be for my next round of transfers? I've done LME and BRF. Looking for something spicyyyy, I know potatoe flakes are kind of vanilla but I may try that next.

Also I have active yeast, I'm pretty sure that was in your first post. You cook it before hand right? but wouldn't thePC kill it and turn it into nutritional yeast?

 

Potato flakes are more beginner friendly than yeast.

 

Is that thing airtight?

 

Yeah I believe so, I have had wayyyyy better luck with those little containers than anything else I have tried including Petris. I usually wrap them with cling wrap but the first few I did without a wrap showed no sign on contamination so I kept doing it. You can get like 100 for 3$. I'd rather toss those than petris for sure

 

I do clean them out before hand with ISO.

 

I'll look into yeast a bit more before testing it out.


Edited by macgyver, 25 November 2019 - 08:05 PM.

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#863 CatsAndBats

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Posted 25 November 2019 - 10:44 PM

 

 

This guy is about to push the lid off....


What should my nute be for my next round of transfers? I've done LME and BRF. Looking for something spicyyyy, I know potatoe flakes are kind of vanilla but I may try that next.

Also I have active yeast, I'm pretty sure that was in your first post. You cook it before hand right? but wouldn't thePC kill it and turn it into nutritional yeast?

 

Potato flakes are more beginner friendly than yeast.

 

Is that thing airtight?

 

Yeah I believe so, I have had wayyyyy better luck with those little containers than anything else I have tried including Petris. I usually wrap them with cling wrap but the first few I did without a wrap showed no sign on contamination so I kept doing it. You can get like 100 for 3$. I'd rather toss those than petris for sure

 

I do clean them out before hand with ISO.

 

I'll look into yeast a bit more before testing it out.

 

 

 

gallery_147940_1513_2684.jpg


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#864 MsBehavin420

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Posted 26 November 2019 - 10:33 AM

Those are a really great
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#865 ElrikEriksson

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Posted 02 December 2019 - 01:10 AM

Question: Just how long do you all wait for spores to germinate before giving up on a set of plates?

I've heard '3 to 30 days' quoted as a germination window a few times. Is there any real possibility of growth after 30 days?



#866 CatsAndBats

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Posted 02 December 2019 - 02:19 AM

Question: Just how long do you all wait for spores to germinate before giving up on a set of plates?

I've heard '3 to 30 days' quoted as a germination window a few times. Is there any real possibility of growth after 30 days?

 

I wait six months.


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#867 ElrikEriksson

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Posted 02 December 2019 - 01:12 PM

Seriously?

Have you actually seen 3 month old plates germinate?


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#868 CatsAndBats

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Posted 02 December 2019 - 01:25 PM

Seriously?

Have you actually seen 3 month old plates germinate?

 

Nope. I think the longest was a month. I got that number from Microbe when I was trying to revive 8+ year old prints.


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#869 happy4nic8r

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Posted 02 December 2019 - 06:07 PM

cat eyes.jpg

 

Did you revive the 8 year old prints? Just curious. I'm about to try to revive the prints I sealed in laminating plastic back when we did the newspaper, Lazlo style grow. I ran low on prints and figured it couldn't hurt to try these.

 

They are from 2013 or 2015, can't read my own scribbling, so either 4 or 6 years old. Done in December so I think that's the perfect timing.

 

 



#870 CatsAndBats

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Posted 03 December 2019 - 09:26 AM

 

 

Did you revive the 8 year old prints? Just curious. I'm about to try to revive the prints I sealed in laminating plastic back when we did the newspaper, Lazlo style grow. I ran low on prints and figured it couldn't hurt to try these.

 

They are from 2013 or 2015, can't read my own scribbling, so either 4 or 6 years old. Done in December so I think that's the perfect timing.

 

 

Negative.



#871 happy4nic8r

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Posted 03 December 2019 - 03:20 PM

Well I'm going to try these out on agar, and after reading most of this posting, I will try a number of mixes, some straight agar with nutes, some with added post agar conditioning blended in and some with ? pass the electrodes Dr Frankenstein. If I can get anything to pop, I'll have my winner for the longest stored spores, and a way to store them in plain sight that is easy and tight.

 

IMG_20191202_1540541.jpg



#872 MsBehavin420

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Posted 04 December 2019 - 10:42 AM

I lost all my agar when my fridge crashed.. Time to make the donuts!

#873 macgyver

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Posted 06 December 2019 - 07:13 PM

Did some transfers from the plate in pic one. Added my future substrate for the first time in this batch - coir, small amount of BRF, egg shells, and kelp meal. Still on LME for the main nutrition, but the myc is loving this batch! Not sure if it is just stronger transfers, or the additives, but the rate of growth is really good. I am loving the red stain, it is easier to view from the bottom of the plate. I did notice that the plates that I poured with more agar had much less condensation, not sure if this is a known phenomenon but I like it. I think I'm going to try out a different recipe next time around without the additives. Just saw the incredibly long list of recipes from earlier in the thread. Decisions, decisions... I think I'm getting closer to a pseudo monoculture.

 

Very exciting stuff :biggrin:

20191125_193004(1).jpg 20191206_134158.jpg 20191206_133859.jpg

EDIT: also these little cups actually can be pressure cooked, but the lids can't :(


Edited by macgyver, 06 December 2019 - 07:19 PM.


#874 MsBehavin420

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Posted 10 December 2019 - 09:00 AM

Pesa
I cant figure out how to send pics thru messages..
Its been over 2 weeks.. Cant get this to fruit....

20191209_212217.jpg

20191209_212245.jpg


20191209_212312.jpg

#875 CatsAndBats

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Posted 01 January 2020 - 09:19 PM

Well I'm going to try these out on agar, and after reading most of this posting, I will try a number of mixes, some straight agar with nutes, some with added post agar conditioning blended in and some with ? pass the electrodes Dr Frankenstein. If I can get anything to pop, I'll have my winner for the longest stored spores, and a way to store them in plain sight that is easy and tight.

 

 

 

Spores def need micronutes to germinate.

 

I lost all my agar when my fridge crashed.. Time to make the donuts!

 

Get at it, Sis.

 

Pesa
I cant figure out how to send pics thru messages..
Its been over 2 weeks.. Cant get this to fruit....

attachicon.gif20191209_212217.jpg

attachicon.gif20191209_212245.jpg


attachicon.gif20191209_212312.jpg

 

Increase o2.
 



#876 MsBehavin420

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Posted 01 January 2020 - 09:41 PM

I posted a new thread about this. Surface started getting weird. Brown discs.

https://mycotopia.ne...s/?fromsearch=1

#877 MsBehavin420

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Posted 01 January 2020 - 09:46 PM

I flipped her over and covered with saran. The bottom wasnt colonized.
i was fanning 3x a day. Moved it near the heat vent bc the room was drafty. My heat vents were disassembled in nov and cleaned and a new heat unit installed that has a UV sterilization on the outgoing air.
Nada. The only thing I can think of is temp.... But its 60(night) -72 (all day and until honey goes to bed at like 3am)

I have a hunk of the agar i tossed on some pasteurized oak(may be some pine mixed in) sawdust from my pallets and egg carton. Its going but sllllooooly

#878 macgyver

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Posted 20 January 2020 - 03:30 PM

Hey in terms of petri dishes, does it matter if they're vented or not?


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#879 RutgerHauer

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Posted 20 January 2020 - 05:02 PM

Not really. On the shroomery RR mentions somewhere that it is important for the mycelium to be able to breathe (duh) and that you really need vented dishes.. I guess the plastic ones are more closed off/tightly fitted than the glass ones, so it might be helpful there. Glass ones are not airtight, the lids fit pretty loosely. I have been getting good results with non vented glass dishes, but if you go polystyrene I would say why not go vented, shouldn't be very much more expensive. Glass ones are hard to find vented.

 

Getting some extra non vented dishes as soon as I can, I am running out.

 

In looking around the fora today I found a post where it is mentioned that 60mm dishes should be more efficient than 100mm dishes in that there is more mycelial growth. I'm not sure of the specifics of it.

I now work with 80mm dishes and find those big enough, but was thinking or going bigger for my new ones. 60mm should be fine too and might be more efficient in the sense that you use less agar as well - of course they will be done quicker too! I think I'll just keep it uniform and stick with 80mm myself.


Edited by RutgerHauer, 20 January 2020 - 05:10 PM.


#880 CatsAndBats

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Posted 20 January 2020 - 05:08 PM

I keep mine sealed.


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