Paradox
©
Fisana

Jump to content


Photo
* * * * * 12 votes

Catattack some Agar 3.0


  • Please log in to reply
1065 replies to this topic

#881 macgyver

macgyver

    Mycotopiate

  • VIP
  • 450 posts

Awards Bar:

Posted 20 January 2020 - 05:10 PM

Not really. On the shroomery RR mentions somewhere that it is important for the mycelium to be able to breathe (duh) and that you really need vented dishes.. I guess the plastic ones are closed off more than the glass ones, so it might be helpful there. Glass ones are not airtight, the lids for pretty loosely. I have been getting good results with non vented glass dishes, but if you go polystyrene I would say why not go vented, shouldn't be very much more expensive. Glass ones are hard to find vented.

 

Getting some extra non vented dishes as soon as I can, I am running out.

 

In looking around the fora today I found a post where it is mentioned that 60mm dishes should be more efficient than 100 mm dishes in that there is more mycelial growth. I'm not sure of the specifics of it.

I now work with 80mm dishes and find those big enough. 60mm should be fine too - of course they will be done quicker too! I think I'll just keep it uniform and stick with 80mm myself.

Ok, cool. I just thought it didn't matter because you will parafilm/saran wrap the sides anyway so there wouldn't be airflow.

 

I ordered like 50 off of a website and accidentally got 60mm, I don't mind them! A little more efficient, especially for cleaning a culture up. I think I would want 90mm or a 100mm to truly see if it is clean/to isolate though.


  • RutgerHauer likes this

#882 RutgerHauer

RutgerHauer

    Knows Nothing

  • Free Member
  • 1,009 posts

Awards Bar:

Posted 20 January 2020 - 05:12 PM

Parafilm breathes. That's why you use parafilm. I'm not sure if saran wrap does. I have 50 right now but I'll settle for a 100 more.


  • CatsAndBats likes this

#883 macgyver

macgyver

    Mycotopiate

  • VIP
  • 450 posts

Awards Bar:

Posted 20 January 2020 - 07:50 PM

Ah I didn't know that! I've been using saran wrap this entire time, without any serious issues, but maybe that's why my older plates are bruising...



#884 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 12,188 posts

Awards Bar:

Posted 20 January 2020 - 11:06 PM

Ah I didn't know that! I've been using saran wrap this entire time, without any serious issues, but maybe that's why my older plates are bruising...

Saran Wrap breathes too as I understand it.

 

Saran (plastic) used to be PVDC (polyvinylidene chloride) which is the least permeable of all plastic film materials by a large factor (see end of paragraph 3 in the section titled History in the link above). However, it is now made of LDPE (low density polyethylene) which has a great deal higher gas permeability. Too bad, the old product really worked and had occasional value in the lab or shop as well.

 

https://www.quora.co...-than-the-other


  • macgyver likes this

#885 macgyver

macgyver

    Mycotopiate

  • VIP
  • 450 posts

Awards Bar:

Posted 20 January 2020 - 11:12 PM

 

Ah I didn't know that! I've been using saran wrap this entire time, without any serious issues, but maybe that's why my older plates are bruising...

Saran Wrap breathes too as I understand it.

 

Saran (plastic) used to be PVDC (polyvinylidene chloride) which is the least permeable of all plastic film materials by a large factor (see end of paragraph 3 in the section titled History in the link above). However, it is now made of LDPE (low density polyethylene) which has a great deal higher gas permeability. Too bad, the old product really worked and had occasional value in the lab or shop as well.

 

https://www.quora.co...-than-the-other

 

I had to read that a few times, so Saran wrap now has LESS gas permeability than it's predecessor. So high gas permeability is a good thing for agar then?



#886 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 12,188 posts

Awards Bar:

Posted 21 January 2020 - 12:00 AM

 

 

Ah I didn't know that! I've been using saran wrap this entire time, without any serious issues, but maybe that's why my older plates are bruising...

Saran Wrap breathes too as I understand it.

 

Saran (plastic) used to be PVDC (polyvinylidene chloride) which is the least permeable of all plastic film materials by a large factor (see end of paragraph 3 in the section titled History in the link above). However, it is now made of LDPE (low density polyethylene) which has a great deal higher gas permeability. Too bad, the old product really worked and had occasional value in the lab or shop as well.

 

https://www.quora.co...-than-the-other

 

I had to read that a few times, so Saran wrap now has LESS gas permeability than it's predecessor. So high gas permeability is a good thing for agar then?

 

Saran wrap does allow for gas exchange, even more now since they changed the formula, which in theory, is good for us and bad for people concerned about food safety/quality. Is how I read that.

 

The greater point being, I don't allow for GE in my no-pours, the myc grows fast enough that I don't worry about o2, as long as I'm transferring to new 'plates' in a timely fashion.


  • bezevo likes this

#887 bezevo

bezevo

    Mycotopiate

  • Gold VIP
  • 895 posts

Awards Bar:

Posted 21 January 2020 - 10:37 AM

glad wrap ......................i was TOLD  .......................by an INTERNET Expert breaths better than Saran .................hummm.........

 

 

any comment EXPERTS


  • MsBehavin420 and itok335 like this

#888 MsBehavin420

MsBehavin420

    Mycotopiate

  • VIP
  • 1,390 posts

Posted 23 January 2020 - 08:39 AM

Parafilm breathes. That's why you use parafilm. I'm not sure if saran wrap does. I have 50 right now but I'll settle for a 100 more.

I believe that its Glad brand that breathes, not Saran. I would look further into this (simply bc i dont remember exactly) and will when i go to the store.
  • itok335 likes this

#889 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 12,188 posts

Awards Bar:

Posted 14 April 2020 - 01:26 PM

post-147940-0-98945000-1586887416.jpg

 

post-147940-0-11729600-1586887422.jpg

 

post-147940-0-37874100-1586887427.jpg

 

Just as a reminder, kids, when you make agar, or LC and plan to germinate spores (germinating spores in LC is not good technique, but I know some of y'all gon do it anyway), always add a "brown" element. Spores need the micronutrients to germinate.

 

This LC is just sucrose and a touch of molasses so y'all can see what it looks like.

 

Standard LC is 4% nutes, so this is 500ml of h2o, so that's 20g sucrose, and a drop or two of molasses. I also added a marble to make it easier to stir.

 

The two other shots are to show y'all that I still use agar. :tongue:

Attached Thumbnails

  • 20200414_132834.jpg
  • 20200414_132828.jpg
  • 20200414_132825.jpg

Edited by CatsAndBats, 14 April 2020 - 01:30 PM.

  • Arathu likes this

#890 Arathu

Arathu

    Dirtmaker

  • OG VIP
  • 6,302 posts

Awards Bar:

Posted 15 April 2020 - 05:53 AM

CatAtives are a fine example of the spice of life......Man I have to get supplies soon......what an excellent use for stimulus checks....IMHO

 

If you're gonna rob the future might as well help make sure that fungal diversity is promoted......among other things..

 

A


  • CatsAndBats and YoshiTrainer like this

#891 CatsAndBats

CatsAndBats

    this motherfucker

  • OG VIP
  • 12,188 posts

Awards Bar:

Posted 17 April 2020 - 12:50 PM

Agar porn, and the value of growing PF cakes.

 

 

post-147940-0-92065800-1587144671.jpg

 

My partner, helping organize. PF classic and Mikey's stable ARX cross.

 

post-147940-0-44973100-1587144677.jpg

 

I love pulling rhizo tissue from the tops of these strands that run up the jar. The stretch informs me that the myc is "determined" to find a new food source and to colonize aggressively, IMHO.

 

post-147940-0-20711200-1587144682.jpgI was having bad luck with a Chitwan syringe that I had ordered by accident, ie inconsistent flushes, slow to colonize, very weak germination rates, etc.

 

So, PF Cakes to the rescue! I made up a bunch of cakes, innoced them, and we have a winner! One cake had a contamination, three never germinated and this one is killing it! I'll fruit it after 100%, grab tissue and/or a print from any clusters! Bam, two (potential) ways to narrow down to good genetics from what began as a shitty syringe. 

 

That's why I preach about the value of cakes!

 

post-147940-0-57666300-1587144692.jpg

 

Same cake a couple of days before.

 

post-147940-0-38712000-1587144687.jpg

 

 

Close up of my very handsome lab assistant!

 

 

Attached Thumbnails

  • 20200415_225008.jpg
  • 20200415_224706.jpg
  • 20200415_224452.jpg
  • 20200416_121723.jpg
  • 20200414_135755.jpg

  • Arathu, ItBeBasidia and YoshiTrainer like this

#892 FLASHINGROOSTER

FLASHINGROOSTER

    Semi-Pro Taco Robot

  • Black VIP
  • 1,805 posts

Awards Bar:

Posted 17 April 2020 - 02:10 PM

I figured out why the say a black cat is bad luck.

 

You can't see the little mofo's when you turn the light off. My poor kitty has taken an accidental kick or two


  • CatsAndBats and Oneyedraven like this

#893 Arathu

Arathu

    Dirtmaker

  • OG VIP
  • 6,302 posts

Awards Bar:

Posted 17 April 2020 - 06:06 PM

Excellent stuff there Cats,nice work.....there's just something about dancing with a rhizome.

 

I like your thinking as to the behavioral characteristics of a mycelium.

 

I have a species of reishi that grew through the filter patch of it's jar and is attempting to jump to a log outside. It's aggressive to say the least. 

 

I do believe we are communicating with them and them with us.

 

Symbiosis for sure.....


  • CatsAndBats likes this

#894 YoshiTrainer

YoshiTrainer

    Onion tied to belt

  • Gold VIP
  • 644 posts

Awards Bar:

Posted 17 April 2020 - 07:08 PM

Great pics and handsome fellow too!

It seems like spores start faster and more consistently on cakes than agar. Maybe it is the soak in the syringe water or the way the spore is surrounded on all sides by the cake? Maybe it is just luck? :)
  • CatsAndBats likes this

#895 FLASHINGROOSTER

FLASHINGROOSTER

    Semi-Pro Taco Robot

  • Black VIP
  • 1,805 posts

Awards Bar:

Posted 18 April 2020 - 08:26 AM

Cool

 

I want some rhizo like that in my jars



#896 Arathu

Arathu

    Dirtmaker

  • OG VIP
  • 6,302 posts

Awards Bar:

Posted 18 April 2020 - 08:33 AM

Cool

 

I want some rhizo like that in my jars

And you can definitely do it to. Try taking transfers from very small area's in front of mycelium on a plate or in a jar that show you the characteristics you are interested in exploring. They definitely can be propagated in that way and one can absolutely spend months and months working on and documenting such experiments. (note YEARS is a more likely unit to use) It's absolutely fascinating to do and explore and it's not possible to run out of avenues for that kind of exploration....IME. 


  • YoshiTrainer likes this

#897 FLASHINGROOSTER

FLASHINGROOSTER

    Semi-Pro Taco Robot

  • Black VIP
  • 1,805 posts

Awards Bar:

Posted 18 April 2020 - 09:36 AM

Yeah I think I have the syringe thing figured out (fingers crossed) with BRF jars so it's my next logical step.  So the idea is to try to transfer the aggressive looking sections to keep propagating those strong genetics? And those being the one's at the edge, looking for food?

 

After much failure I am just enjoying watching my cakes grow, although my first cake flush was a little lacking in comparison to some of the awesome cakes I have seen out there. I mean who wouldn't want one of those bad boys


  • Arathu likes this

#898 Arathu

Arathu

    Dirtmaker

  • OG VIP
  • 6,302 posts

Awards Bar:

Posted 18 April 2020 - 11:09 AM

Yeah I think I have the syringe thing figured out (fingers crossed) with BRF jars so it's my next logical step.  So the idea is to try to transfer the aggressive looking sections to keep propagating those strong genetics? And those being the one's at the edge, looking for food?

 

After much failure I am just enjoying watching my cakes grow, although my first cake flush was a little lacking in comparison to some of the awesome cakes I have seen out there. I mean who wouldn't want one of those bad boys

Yes, we begin to either recognize or just simply want to explore some part of the mycelium that demonstrates a set of characteristics we "think" will lead to fruiting....aggressive rhizome is known to produce fruits.  


  • FLASHINGROOSTER likes this

#899 Dabluebonic

Dabluebonic

    Mycotopiate

  • Free Member
  • 314 posts

Posted 18 April 2020 - 01:29 PM

Dumb question, but taking prints from nice clusters helps narrow down genetics? I assumed all MS was random.
  • CatsAndBats likes this

#900 Arathu

Arathu

    Dirtmaker

  • OG VIP
  • 6,302 posts

Awards Bar:

Posted 18 April 2020 - 01:47 PM

I am definitely speaking about agar work and isolation of mycelium that "I HOPE" have great genetics based on the characteristics and behavior on agar. Isolation of specific genetics like clustering I do with a clone from one of the fruits....yes MS is a wild west shootout. 

 

NOT a dumb question at all....

 


  • CatsAndBats likes this




Like Mycotopia? Become a member today!