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blue tint to mycelium. contaminant or not?


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#1 orangutan

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Posted 08 February 2017 - 12:53 AM

I started some spores on agar and got beautiful growth.  In the center, densest part, I noticed a blue tint to the mycelium, down in the bottom.

 

It doesn't seem to be spreading.  Could it be some kind of mold or is it acutally the mycelium?

 

Screen Shot 2017-02-07 at 9.40.43 PM.png Screen Shot 2017-02-07 at 9.41.53 PM.png

 

I'm not taking any risks, so I transferred a few clean edges of mycelium to a new plate.  Here you can see where one or two circles of mycelium are doing the same thing.

 

Screen Shot 2017-02-07 at 9.42.53 PM.png

 

I am going to keep transferring until I get something pure white.  But I am curious... is this even contamination?  It's not spreading or taking over.  It doesn't seem to be competing.  And it looks like it is coming from deep inside. 

 

What do yall think?

 

 


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#2 fahtster

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Posted 08 February 2017 - 01:36 AM

Just looks like bruising to me, but it's weird that it's just coming from the center. Or maybe it isn't.. I haven't done any agar. My vote is bruising... is it really dry in the jar?

Faht

Edited by fahtster, 08 February 2017 - 01:37 AM.

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#3 MLBjammer

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Posted 08 February 2017 - 04:56 AM

It looks like bruising to me as well.  Mold on agar will get nasty pretty fast most of the time.


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#4 Arathu

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Posted 08 February 2017 - 07:57 AM

I'm gonna have to third that..........it looks like bruising to me as well............but now my inquiring mind wants to know why? (I usually get in all kinds interesting situations after I ask why?) :biggrin:

 

 

A


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#5 orangutan

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Posted 08 February 2017 - 11:39 AM

I don't know why!  It is not dry.  The agar actually had a little liquid water on the surface from condensation when I first put the spores on it.

 

Maybe the spores were so dense that they produced way too many hyphae that all squeezed together so much that they bruised?

 

I sacrificed one of those places in the name of science.  I scratched the area where I saw blue and there was no mold.  The mycelium aggregated into itself and turned even more blue.  



#6 MLBjammer

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Posted 08 February 2017 - 12:27 PM

I have had that happen to me. My thoughts were along the same line. Maybe one of the agar masters will chime in.
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#7 themickster

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Posted 09 February 2017 - 02:40 AM

I just had the same thing happen. Although my plate was contaminated.
When the mycelium ran up against the contam, it started bruising.
In the photo you can see where I took a small wedge early on in its growth. I may have contaminated it then. Always neat to see how these things work out. I've been known to keep a sealed up plate that's contaminated just to see it progress.

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#8 Arathu

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Posted 10 February 2017 - 06:56 PM

Fungal and other life forms like this are amazing to observe IMHO..........it looks like they are doing war at the front! Probably because they are.......... :biggrin:


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#9 coorsmikey

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Posted 10 February 2017 - 07:06 PM

Bluing while on agar is usually associated with certain strains. Just so happens that most of those certain strains are arguably more potent. Do you mind me asking what strain you have there?


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#10 orangutan

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Posted 10 February 2017 - 10:09 PM

funny you should ask, @coorsmikey.  That is a Redboy that I got from a very kind fellow 'topiate!!

 

Screen Shot 2017-02-10 at 7.08.24 PM.png


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#11 coorsmikey

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Posted 10 February 2017 - 10:41 PM

Small world it is! You will enjoy those!

Edited by coorsmikey, 11 February 2017 - 08:33 AM.

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#12 themickster

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Posted 10 February 2017 - 11:57 PM

The one that bruised on me is Orissa India, which totally kicked my ass to blackout on 3.5 carefully weighed grams. They were bottle grown with a super long consolidation time and produced very dense fruits.
I may have crossed to the other side on that trip..... :)

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#13 fahtster

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Posted 11 February 2017 - 12:06 AM

Hard to argue with that. :)

Faht
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#14 peacefrog

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Posted 11 February 2017 - 05:44 AM

I commonly see blueing on older agar dishes stored in the ref. due to drying out. IMO you have just bruising, which could be like mikey said, just strain tendencies, you bruised it while transferring, something's not quite perfect for the mycelium, or it is fighting a contam.
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#15 orangutan

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Posted 11 February 2017 - 04:14 PM

Thanks everyone..

 

Whatever it is, they mycelium is on to "browner" pastures and just got transferred to birdseed and no problems yet  :biggrin:

 

My agar mix was

 

10g agar,

10g malt extract,

1g tryptone peptone

 

The "tryptone peptone" came from a very old bottle of bacteriological supplies I had access to.

 

Anyway, as long as I get some growth on agar I'm not really stressing about what is in it.  I just wanted to get spores to germinate and get them moved to grain and beyond.  The real "beef" is in the fruiting substrate, not the agar.

 

thanks for reading and commenting


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#16 MLBjammer

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Posted 12 February 2017 - 06:23 AM

This is a good thread for agar portion of the New Vaults.


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