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My first PF Tek grow- P. cubensis GT


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#1 tailsmcsnails

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Posted 13 February 2017 - 08:00 PM

I hope this is in the right place, sorry if it is not!

 

14/2/17

My P. cubensis Golden Teacher spores arrived (syinges) yesterday so I got to make up my jars and sterilise them in the PC.

 

IMG20170213205104.jpg

 

After work today I will inoculate and then the fun (trouble?) will start!  I wish I had some agar so I could test the syringe first but I don't so we shall see what happens.

 

As a complete noob I am planning to follow the venerable PF tek more or less to the letter, hoping for smooth sailing.

 

 


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#2 Arathu

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Posted 13 February 2017 - 08:26 PM

Yes...and a very wise choice of strain and path to follow forwards IMHO.........PF rules............. patience and your myco family are great allies in your development.

 

Sending good healthy growth vibes to you and yours..........looking forward to your success..........and PORN....did I mention we LOVE SHROOMY PORN

 

A


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#3 tailsmcsnails

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Posted 13 February 2017 - 08:28 PM

Yes...and a very wise choice of strain and path to follow forwards IMHO.........PF rules............. patience and your myco family are great allies in your development.

Sending good healthy growth vibes to you and yours..........looking forward to your success..........and PORN....did I mention we LOVE SHROOMY PORN

A

You guys are the best, always make me smile. Porn ahoy!
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#4 tailsmcsnails

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Posted 14 February 2017 - 04:09 AM

I cleaned myself and my room and got all set up in my rudimentary glove box to inject my spores.  Talk about being all thumbs!! Haha I guess if it was easy, everyone would be doing it.  I hate to think how many inadvertently un-aseptic things I did!  Anyway my jars are inoculated, hopefully only with GT spores!  

 

The jars are in a plastic bin, and it's summer here so I haven't done any kind of heating.  I'm going to read the tek through and make sure I haven't missed anything.

 

We shall seeeee.


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#5 MLBjammer

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Posted 14 February 2017 - 04:29 AM

I bet your jars are just fine.  And room temps are all you need.

 

Good luck, and keep us posted.


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#6 CatsAndBats

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Posted 14 February 2017 - 09:26 AM

I cleaned myself and my room and got all set up in my rudimentary glove box to inject my spores.  Talk about being all thumbs!! Haha I guess if it was easy, everyone would be doing it.  I hate to think how many inadvertently un-aseptic things I did!  Anyway my jars are inoculated, hopefully only with GT spores!  

 

The jars are in a plastic bin, and it's summer here so I haven't done any kind of heating.  I'm going to read the tek through and make sure I haven't missed anything.

 

We shall seeeee.

 

 

I bet your jars are just fine.  And room temps are all you need.

 

Good luck, and keep us posted.

 

 

 

Just say no to added heat/incubation. IMHO.  "un-aseptic" haha! good stuff. The more stuff that one does in an SAB/GB the better. The more comfortable one is the sharper one's skills will become, especially when precision becomes more critical (agar).


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#7 tailsmcsnails

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Posted 14 February 2017 - 06:40 PM

Everything was in the Sab.... Including possibly a lot of cooties!

Funny you mention agar because I was literally laughing as I recalled my agar ambitions of earlier in the day...
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#8 fahtster

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Posted 15 February 2017 - 03:17 AM

Good luck!

Faht
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#9 tailsmcsnails

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Posted 16 February 2017 - 01:34 AM

This is what they look like today, after about 48 hours...

 

shroom1.jpg

 

shroom2.jpg

 


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#10 CatsAndBats

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Posted 16 February 2017 - 07:53 AM

@tails, did you do 2:1:1 ?



#11 MLBjammer

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Posted 16 February 2017 - 12:29 PM

The bottoms of those jars look pretty wet. They might be all right, but it will slow colonization down somewhat.

#12 tailsmcsnails

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Posted 16 February 2017 - 04:44 PM

The bottoms of those jars look pretty wet. They might be all right, but it will slow colonization down somewhat.

I actually was worried that the mix seemed a bit dry when I put it in, but I stuck to the recipe.

 

I'm thinking that my vermiculite is a bit coarse and I should probably crush it up a bit finer next time to try and keep the rice flour from settling out?

 

Do you think I need to do anything about my current jars or is it just a matter of wait and see?



#13 tailsmcsnails

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Posted 16 February 2017 - 04:45 PM

@tails, did you do 2:1:1 ?

yes.

 

What do you see?



#14 CatsAndBats

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Posted 16 February 2017 - 04:50 PM

I have no idea where the idea of different sized vermiculite being good or bad came from but I don't believe any of it.

 

I don't know what I see. Is there a dry verm layer? Just looks real white below the top layer I guess.



#15 tailsmcsnails

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Posted 16 February 2017 - 05:09 PM

I have no idea where the idea of different sized vermiculite being good or bad came from but I don't believe any of it.

 

I don't know what I see. Is there a dry verm layer? Just looks real white below the top layer I guess.

That was just my own idea because it seems like the rice flour has kind of settled out... I'm all for simple so I won't bother if you think it's not important.

 

Yes, the dry verm layer is visible at the top of the jars.  You can see it better in the enlargedish pic.  The fuzzy whiteness is only in the BRF+ verm layer.



#16 CatsAndBats

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Posted 16 February 2017 - 05:17 PM

If one reads older PF teks there is a lot of debate on what 'grade' the verm should be that's all.


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#17 Arathu

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Posted 17 February 2017 - 10:41 AM

Never heavily packed (although Hyphaenation's puck tek says different but that ain't PF tek) and never too wet (which usually causes bacterial issues)...............other than that you'll be amazed at what the fungi do.........

 

Courser verm was touted as a way to avoid heavy packing of the jar methinks but I've seen all grades of verm work.... loosing the consistency and distribution of nutrients vs moisture holding non nutritional substrate could be troublesome but now you'll see that in action.....If your rice has fallen into a wet layer at the bottom, I can't tell from the pictures .....make notes for yourself............ :cool:

 

Still good vibes coming atcha..........

 

A



#18 tailsmcsnails

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Posted 18 February 2017 - 05:46 AM

Never heavily packed (although Hyphaenation's puck tek says different but that ain't PF tek) and never too wet (which usually causes bacterial issues)...............other than that you'll be amazed at what the fungi do.........

 

Courser verm was touted as a way to avoid heavy packing of the jar methinks but I've seen all grades of verm work.... loosing the consistency and distribution of nutrients vs moisture holding non nutritional substrate could be troublesome but now you'll see that in action.....If your rice has fallen into a wet layer at the bottom, I can't tell from the pictures .....make notes for yourself............ :cool:

 

Still good vibes coming atcha..........

 

A

Some BRF has filtered to the bottom but not all, and I don't think it's that wet... live and learn I guess, we shall see thanks for the vibes!


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#19 peacefrog

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Posted 18 February 2017 - 09:05 AM

I personally don't trust a recipe for field capacity. When I make cakes, I always add water slowly and feel with my hands if there is enough water. Just my preference. I always use LI for cakes so I tend to hold back a little water from the mix so I can add a lot of inoculant without messing up the water content of the cakes.

IMO, slightly on the drier side of field capacity is perfect for cakes. If fruiting as cakes or crumble and case, you can always dunk after birthing. Or if using as spawn, you can dunk before or just spawn as is to whatever substrate you perfer that has been brought to full field capicity and all will be fine.
My 2 pennies.

Edited by peacefrog, 18 February 2017 - 12:39 PM.

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#20 MLBjammer

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Posted 18 February 2017 - 06:01 PM

Yeah, always add water slowly to your cake mix and squeeze it along the way until only a few drips fall from your closed fist.  That's when it's good to go.  Also keep stirring even as you load your jars, as water likes to settle in the bottom of a mix.

 

And, as far as the size of vermiculite, it really makes no difference.  I actually prefer the finer grade stuff, especially for a dry vermiculite barrier (as the smaller pieces filter more air).

 

It could be the lighting in that picture, Tails, that makes me think the jars look wet.  I am sure you will update with more pics soon.

 

Good vibes.


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