Posted 29 April 2017 - 12:58 AM
@Arathru you said it accurately, Amateurs and jelly jars. You wont go to a lab and find scientist using jelly jars and believe you me, these lab teks and scientist know about our amatuer teks lmao.
Now this is not a attempt to insult or throw stones, im simply a amateur myco myself who always tries to play with the pros ;)
Jelly jars are best for SAB's for sure. Not saying one cant pull off plates in a SAB, im just saying there are pro's and cons as there are with plates.
Plates are ideal for flow hoods but also have pros and cons.
IMO using jelly jars is handicapping someone who cant execute a more of a 'lab' process. Similar to tossing verm in the bottom of a grain jar. Absolutely no difference what so ever. It is simply a fail safe method which requires minimal technicue.
Now where i like the jelly jars is if you were going to harvest the mycelium from the agar surface or use @hyphenation popcorn/agar tek. Jars hands down destroy plates for that application.
Because there is no disciplined technicue, respectively speaking, it is very easy to produce clean nutrient agar. Hence @Amatuers. I love amatuer porn btw, especially when a hot Jewish chick stores her anal beads in a jelly jar because i know the strand had been sterilized! Wait i should not have said that, why would i care if they were sterilized or not, they are not for me, im sorry just forget i said any of that and please whomever reads this, tell noone!
So now the plates. Dang i already used up my mojo on that amatuer porn thing up there^.
1st off the view is much better, i dont care if someone sais they can see their cultures just fine. If you have a wild multi genetics running all over the place, you cant even come close to viewing what you can in a a jelly jar as a plate unless you pop the lid off.
If your working with a iso then no need to be able to look down and examine.
Manipulation of the lid, man this is self explanatory, you can control the amount of culture exposed while fluently inserting your utensil with precision. With a jelly jar you have to remove the lid completely or almost but you stick a heck of a lot more stainless in there and there is no way anyone will convince me that you can have the same accuracy of selection and fluency as you would with a plate, no way.
Plates may have a larger foot print unless you use 65's like i did/do i guess, but plates stack vertically to greater heights allowing for more culture per square inch storage in a specified space. Whoopy no big deal i right.
Condensation is much easier delt with glass to glass then the heat transfer from metal lid to glass. I have the highest level certification of Firefighting in my state which required a degree in fire science/thermal physics was a must. However that was 20 years ago and just like most other training required for the job such as Paramedics, we only used 90% of what we were required to know. Whoa off track my friends. There is also more surface area of glass further away from the warm agar surface exposed to ambient air/temps, condensation is more of a challenge to combat.
Plates just me feel better about myself. They make me feel like a scientist and im ready to write a book.....
Joking! My grammar is to horrible to write a book.....
I have a
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