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Fuzzy's First Agar Adventure


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#61 CatsAndBats

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Posted 19 April 2017 - 10:16 AM

  • admin please do not remove anything @arathu posted
  • admin please change @sandman's name back to @sandyVagina
  • admin please change my name to @batsAndCats instead of @catsAndBats
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  • admin please make bullet points a requirement in every post

Edited by CatsAndBats, 19 April 2017 - 10:18 AM.

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#62 Arathu

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Posted 19 April 2017 - 10:24 AM

Hahahahahahahahahaha...................holy shit I did spit my coffee out...............fucking brilliant!

 

A


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#63 CatsAndBats

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Posted 19 April 2017 - 10:31 AM

..............fucking brilliant!

 

 

 

You are!



#64 tailsmcsnails

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Posted 19 April 2017 - 06:30 PM

Hahaha I came here specifically to demand mandatory bullet points but I was too slow
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#65 fuzzyfunguy

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Posted 20 April 2017 - 04:12 AM

*Omg its 5 am and I almost peeded myself
*I've been pouring in disposable plates latley
*I like the idea of waiting until just the right temp for the agar to cool
*I store freshly poured plates in the fridge, I'm assuming this will cause condensation issues?
*I don't like stacking due to accidently removing lids a lot
*Mmmmmmmm, slants......


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#66 CatsAndBats

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Posted 20 April 2017 - 06:35 AM

*I store freshly poured plates in the fridge, I'm assuming this will cause condensation issues?

 

 

That'll do it.


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#67 sandman

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Posted 20 April 2017 - 08:20 AM

funny_yep_theres_your_problem_you_need_a


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#68 CatsAndBats

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Posted 20 April 2017 - 08:36 AM

funny_yep_theres_your_problem_you_need_a

Theres_Your_Problem1258.jpg


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#69 fuzzyfunguy

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Posted 20 April 2017 - 09:03 AM

I mean obviously it'd cause condensation I just didn't think it would interfere with the growth as much as it has, a valid learning experience, I'll store them on a clean shelf double wrapped in parafilm from now on.

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#70 sandman

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Posted 20 April 2017 - 09:18 AM

You can also just store you plates in ziplock bags unwrapped, in and out in the hood of course after an alcohol wipe down of the bag.


Edited by sandman, 20 April 2017 - 09:21 AM.

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#71 Arathu

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Posted 20 April 2017 - 11:33 AM

Stability of conditions has proven important in this hobby, understanding that along with patience and a repeated standardized set of procedures that work for me has brought success...........

 

Each must find their niche.........that is certainly what the fungi are doing........IMHO

 

A


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#72 fuzzyfunguy

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Posted 27 April 2017 - 07:35 PM

I gave up on the close up shots as the camera on my phone wasn't cooperating but this is more of an accurate representation of the progress I've made so far what do you guys think? 5d992aa922736c3ecbbfa0711261e84b.jpgab26cd6a308d2b52a9e7e4ddb8c92846.jpgfde4be788ba34e65e83a53ca2679756a.jpg

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#73 CatsAndBats

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Posted 27 April 2017 - 08:30 PM

Strain, date, etc.. C'mon man, you know how we do!  1f408.png :biggrin:

They look gorgeous by the way. Nice even healthy uniform radial growth, no signs of contamination. Give those a week or two and you'll have healthy inoculant IMHO. What's next captain?


Edited by CatsAndBats, 27 April 2017 - 08:31 PM.

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#74 Arathu

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Posted 27 April 2017 - 08:37 PM

Dude.........hell yeah!

 

What the Cat said............very nice indeed........

 

Great job fuzzy......... :thumbs_up:

 

A


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#75 fuzzyfunguy

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Posted 27 April 2017 - 09:03 PM

A+ "Albino" Innoculated on 4/24 4th transfer from spore print

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#76 fuzzyfunguy

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Posted 27 April 2017 - 09:09 PM

Strain, date, etc.. C'mon man, you know how we do! 1f408.png
They look gorgeous by the way. Nice even healthy uniform radial growth, no signs of contamination. Give those a week or two and you'll have healthy inoculant IMHO. What's next captain?

Next is to take the more even ones (do any look like mono cultures?) and see how they do on some grain, and isolate the obviously uneven ones with another transfer. After that compare how the different isolates grow on grain spawn and how it grows and fruits on a small bulk substrate, then clone a fruit body from the most promising cluster and get that going in some monotubs, all the while shooting for that strong even pinset.

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#77 fuzzyfunguy

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Posted 27 April 2017 - 09:11 PM

Strain, date, etc.. C'mon man, you know how we do! 1f408.png
They look gorgeous by the way. Nice even healthy uniform radial growth, no signs of contamination. Give those a week or two and you'll have healthy inoculant IMHO. What's next captain?

I'm still a newb at agar, do you mean give them a week or two to grow on the plates and inoculate using a wedge from said plates?

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#78 CatsAndBats

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Posted 27 April 2017 - 09:59 PM

Next is to take the more even ones (do any look like mono cultures?) and see how they do on some grain, and isolate the obviously uneven ones with another transfer. After that compare how the different isolates grow on grain spawn and how it grows and fruits on a small bulk substrate, then clone a fruit body from the most promising cluster and get that going in some monotubs, all the while shooting for that strong even pinset.

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They look pretty isolated out to me, if you want to verify this, make another transfer with two pieces on the same plate and see if they merge seamlessly. Let me be super clear, your excellent scalpel work and your clean technique are very evident. Well done sir, well done!

 

I'm still a newb at agar, do you mean give them a week or two to grow on the plates and inoculate using a wedge from said plates?

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I'm saying based on when inoculated, those plates will be to the edge of the agar surface in approx 4-7 days and will be ripe to be used as inoculant for grain spawn. Clean, radial, rapid growth, all terrific signs to move to grain.

 

Good stuff.


Edited by CatsAndBats, 27 April 2017 - 10:01 PM.

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#79 fuzzyfunguy

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Posted 27 April 2017 - 10:36 PM

Next is to take the more even ones (do any look like mono cultures?) and see how they do on some grain, and isolate the obviously uneven ones with another transfer. After that compare how the different isolates grow on grain spawn and how it grows and fruits on a small bulk substrate, then clone a fruit body from the most promising cluster and get that going in some monotubs, all the while shooting for that strong even pinset.

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They look pretty isolated out to me, if you want to verify this, make another transfer with two pieces on the same plate and see if they merge seamlessly. Let me be super clear, your excellent scalpel work and your clean technique are very evident. Well done sir, well done!

I'm still a newb at agar, do you mean give them a week or two to grow on the plates and inoculate using a wedge from said plates?

Sent from my SM-G900T using Tapatalk


I'm saying based on when inoculated, those plates will be to the edge of the agar surface in approx 4-7 days and will be ripe to be used as inoculant for grain spawn. Clean, radial, rapid growth, all terrific signs to move to grain.

Good stuff.
Yay!!! That's refreshing to hear and a good tip when I want to be sure, thanks Cat :)

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#80 Microbe

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Posted 29 April 2017 - 12:58 AM

@Arathru you said it accurately, Amateurs and jelly jars. You wont go to a lab and find scientist using jelly jars and believe you me, these lab teks and scientist know about our amatuer teks lmao.

Now this is not a attempt to insult or throw stones, im simply a amateur myco myself who always tries to play with the pros ;)

Jelly jars are best for SAB's for sure. Not saying one cant pull off plates in a SAB, im just saying there are pro's and cons as there are with plates.

Plates are ideal for flow hoods but also have pros and cons.

IMO using jelly jars is handicapping someone who cant execute a more of a 'lab' process. Similar to tossing verm in the bottom of a grain jar. Absolutely no difference what so ever. It is simply a fail safe method which requires minimal technicue.

Now where i like the jelly jars is if you were going to harvest the mycelium from the agar surface or use @hyphenation popcorn/agar tek. Jars hands down destroy plates for that application.

Because there is no disciplined technicue, respectively speaking, it is very easy to produce clean nutrient agar. Hence @Amatuers. I love amatuer porn btw, especially when a hot Jewish chick stores her anal beads in a jelly jar because i know the strand had been sterilized! Wait i should not have said that, why would i care if they were sterilized or not, they are not for me, im sorry just forget i said any of that and please whomever reads this, tell noone!

So now the plates. Dang i already used up my mojo on that amatuer porn thing up there^.

1st off the view is much better, i dont care if someone sais they can see their cultures just fine. If you have a wild multi genetics running all over the place, you cant even come close to viewing what you can in a a jelly jar as a plate unless you pop the lid off.

If your working with a iso then no need to be able to look down and examine.

Manipulation of the lid, man this is self explanatory, you can control the amount of culture exposed while fluently inserting your utensil with precision. With a jelly jar you have to remove the lid completely or almost but you stick a heck of a lot more stainless in there and there is no way anyone will convince me that you can have the same accuracy of selection and fluency as you would with a plate, no way.

Plates may have a larger foot print unless you use 65's like i did/do i guess, but plates stack vertically to greater heights allowing for more culture per square inch storage in a specified space. Whoopy no big deal i right.

Condensation is much easier delt with glass to glass then the heat transfer from metal lid to glass. I have the highest level certification of Firefighting in my state which required a degree in fire science/thermal physics was a must. However that was 20 years ago and just like most other training required for the job such as Paramedics, we only used 90% of what we were required to know. Whoa off track my friends. There is also more surface area of glass further away from the warm agar surface exposed to ambient air/temps, condensation is more of a challenge to combat.

Plates just me feel better about myself. They make me feel like a scientist and im ready to write a book.....

Joking! My grammar is to horrible to write a book.....

I have a
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