33 replies to this topic

[Lazlo]

Have you done any sterile work with the flowhood?

[wayback]

Nice work. Iwould love to get the funding and time necessary to build one. The risks of working with still undiscovered contamed jars and cultures could not help but increase one's success rate.

[Hippie3]

what is the cubic feet per minute flow on your fan ?
what is the static resistance of the hepa filter ?

[sandman]

1250 CFM fan and the w.g. pressure drop of the hepa is 100fpm @ 0.74max

[tbonus]

DUDE YOUR THE COOLEST MOFO ROUND THESE PARTS

[Lazlo]

Hey Sandman. Have you done any sterile work with the hood yet?

[sandman]

besides the botched print attempt I did a g2g about 5 days ago. I found out there is a zone of turbulence arround the 2" or so of the side walls AFTER doing this and the bag I used to g2g 8 more bags was sitting directly in this area of turbulance for like 5 minutes lol so 8 of the 10 bags contammed that I g2g in that procedure. The 2 that didnt contam were g2g at the same time as other 8, just used a quart jar for spawn on those 2 so I know the hood is working just to avoid the 2" or so arround the walls.

I just finished right now this very second doing some LC jars. Opened all the jars up, scraped in spores directly from a print, and shook em up in the flowhood. It was fun. 12 1/2 pint jars of karo and distilled water with self healing injection lids ( i know I could have just shot a syringe in to them with no hood but then I would have to make a syringe...)

[Hippie3]

how'd that turn out ?

[dial8]

I thought you could. You just have to transfer up wind. Am I right?

The benefits of a GB over a flowhood:
You can work with contaminated cultures, to transfer to clean dishes. Cant do this in a flowhood cause it blows the contams everywhere....

[Lazlo]

You can transfer upwind from the contaminated culture, but the spores still blow all over you and everywhere alse in the mean while.

[StroFun]

If you make the flow hood end with the filter(no walls for the work area) will that take care of any turbulence in the work area?

[Hippie3]

eh, only in the center
i got better results after i enclosed my unit

[sandman]

the turbulence is only 1.5-2" between each side wall so its not so bad. I just use that area to hold spray bottles and nother non sterile tools.

[siam_jim]

sandman you out did yourself once again...great hood! my hat off to you sir.


siam