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My first Grow -Brazil Cubensis


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#1 Triptomemes

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Posted 09 March 2017 - 12:48 AM

Hello Mycofamily, the last few months have been very exciting for me. I finally joined this site, then went full force into the PF tek. I was really nervous about sterile technique and growing in general but I'm SO glad I gave this a try. This has to be one of the most rewarding, and humbling experience/hobby I've ever done. I only had to post one question throughout the entire grow, because everything I wanted to know had already been asked and answered in incredible detail. Thank you so much Mycotopia, I look forward to growing with this community.

 

 

Now I love reading the grow stories of others, so I decided from the start to keep a journal and pictures of each stage. I'm going to do my best to recapture the fun I've had over the last 2 months. I hope you enjoy it as well.

 

1/26/17

 

I inoculated 5 BRF jars with 1cc each from a MS syringe of Brazil Cubensis.20170125_223359.jpg

They are placed in a cabinet above the fridge to incubate at 73-75F

I was very determined to stretch my syringe through a LC so I used 2cc in a Honey LC(300ml)  and 2cc in a Karo LC(300ml). They are being incubated in my closet at 77-81F

 

1/29/17 

 

I had 1cc left in the Brazil MS syringe so I noced up one more jar(#6).  I wanted to try out an airport lid with a vermiculite contamination layer just to see how it performed.20170210_105335.jpg I figured from the start that it would colonize slowly because of 1 inoculation point so I swirled the syringe as I injected the spore solution. I placed this jar in my closet to compare the effect of Temperature on colonization time.The closet was 77-81F

 

2/1/17

First signs of mycelium growth on jars # 1-5 20170128_200727.jpg

Jar #6 also showing growth today.20170201_113904.jpg There were clumps of black dots in the syringe when I inoculated this jar, so I believe the spores had already germinated, thus speeding up its growth.

 

2/3/17

Jars #1-5 are doing well showing increased growth. Jar #4 (middle jar) has shown the most progress.( I should have put them in order but just for reference the jars are in order from left to right as  #1,2,4,3,5) 20170131_120631.jpg

Jar #6 isn't moving as slow as I thought it would 20170203_091444.jpg

 

2/5/17

The Honey LC grew so much faster than the Karo so I took 4 10cc syringes and collected 40cc's of it. 20170203_091403.jpg

I wanted to test out the LC so I used a tall 1/2 pint jar with 5 holes( 4 around the rim and 1 in the middle) and 1" of dry verm contamination filter. I injected 2cc into this jar (#7)20170205_195659.jpg It was also placed in the closet.

 

2/6/17

Jars # 1,2 and 4 look about 75% colonized while #5 and #3 are near 50%. 20170206_083750.jpg  This being my first grow I'm not sure what fast or slow mycelial growth is, but I'm happy with what I see. 

 

2/8/17

Jars #6 and #7 are coming along nicely. #6 has expanded its hold on the substrate20170208_085945.jpg #7 is showing growth at all the innoculation points 20170208_090103.jpg . I wonder If the 5th hole in the center will help it reach 100% colonization faster.

 

2/10/17

I have to leave for a few days so this will be last check on the jars until the 15th.

Jars #1,2 and 4 are about 90% colonized. Jars # 3 and 5 are around 80%.20170210_104339.jpg

Jar #6 is about 50%20170210_105555.jpg

Jar #7 is growing fast. The different mycelial strands are about to connect with each other on this side. 20170212_102032.jpg

 

2/15/17

Jars #1-5 are fully colonized and now will sit for a week of consolidation before moving to my SGFC. Jar # 6 is at 80% with a few places left uncolonized.20170219_224845.jpg I believe jar #7 is at 100%20170219_224852.jpg The lesson learned here is to never use only 1 inoculation hole. After reading MLBjammers Tek on in vitro quart jars I've decided to fruit #6 and 7 in vitro.

 

2/21/17

PINS!! Jar #6 is showing pins on the side where it was inoculated. Its still not fully colonized on the back side, but within 95%20170221_205201.jpg

 

2/22/17 

Jars #1-5 have been dunked and placed in the fridge. I have pins growing from cake #4 All the cakes smelled fresh and earthy.

 

2/23/17

I rolled the cakes in vermiculite and placed them in the SGFC. The chamber has 3 inches of perlite and the temp is 72-76F with a RH of 85%. I'm fanning and misting every 3 to 6 hours.20170223_091614.jpg

 

2/24/17

The cake from jar #4 has 3 pins which have been growing throughout the day20170225_205841.jpg . The pins in cake # 6 are getting longer20170224_115751.jpg I'm going to dunk it along with cake #7 tomorrow morning.

 

2/26/17 

 Today was "interesting". I started by placing cake #6  into a quart ziplock jars with moist verm under it.20170226_095139.jpg Then by 1:21pm I was having issues with my RH staying near 88-90%  in the SGFC, so I added another inch of perlite and re hydrated it. This went incredibly wrong when I placed the FC back in position only to watch about two cups  of water leak out from the holes. I thought I had  drained the perlite well( Clearly not). Sooo I ended up getting another sterlite container and rebuilding my chamber all evening.  I dunked cakes # 1,2,3, and 5  so they wouldn't dry out and placed cake #4 in a ziplock quart jar. The other cakes dunked for 6 hours and then were moved into the new SGFC with 5inches of perlite;a RH of 95-97% and Temp  of 68-74F.  At 10:15pm I harvested my first mushrooms from cake #4 20170226_222905.jpg This made my whole day and got me even more excited for the rest of the cakes.

 

2/28/17

I DEC jar #6 to help with water retention. I covered up this little guy on the top 20170226_095251.jpg . I hope he will be ok under the wet verm. I've also dunked and DEC jar #4 and placed it into a quart ziplock jar for invitro growing. The new SGFC is doing much better with RH of 97-99% and Temps are 70-75F. The cakes in this fruiting chamber are # 1,2,3 ,5 and 7. Cake #7 is the only one showing pins at this time.

 

3/1/17

Around 1pm I look at cake #6 and see all this fuzzy/hairy white stuff at the base of the fruits20170228_123709.jpg . So in a panic I quarantine it to another room and start asking questions on the site. In my impatience I assumed it was cobweb and treated the fuzzy feet with H202. Now I understand that this is completely normal mycelium formed from lack of FAE. I then took a 1/8th drill bit and added two more holes to the ziplock jar lid. I find that 5-6 holes is working better along with running the ceiling fan 3-4 times a day to move the air around.

 

3/2/17

The veil broke on this chubby guy from cake #620170302_105559.jpg  but I didn't want to remove the cluster so I just removed the cap and left the immature mushrooms to grow. This would of been fine but 3 hours later there was a very sour smell coming from the jars so I removed the whole cluster20170302_204420.jpg . At the end of the day I've harvested 21.8g from two cakes.

 

3/3/17

I harvested a 4g fruit from cake #7 this morning and picked the last fruit from cake #6's first flush. 20170303_092658.jpg Anyone else see the heart?(btw this was the little guy from 2/28/17)

 

3/4/17

I've moved all the cakes out of the SGFC  today. I realized that Its not the best choice for me right now and I don't have the space or room to support it. Instead all the cakes are placed into individual quart ziplock jars. Cakes #1,2,3,and 5 still have no pins. Hopefully I'll have better results with individual humidities and FAE. Cake #4 was dunked and DEC again and now I have this odd growth coming off the side20170304_071238.jpg . I'm sure its a mushroom because I see gills but Its so small I'm not sure what to think. I'll just continue to watch it grow and take pics.

 

3/5/17 -3/6/17

I focused my attention on the mutation on cake #4.20170305_074852.jpg 20170306_063409.jpg 20170306_151618.jpg 20170306_151933.jpg It was so different I barely paid much attention to everyone else.

 

Cake #7 is fruiting nicely. 20170306_093456.jpg The fruits are small but size wasn't my goal. Just actually growing something is more than satisfying.

Cake #6 is on flush 2 and there's 1 fruit coming from the side20170307_182447.jpg

 

3/8/17

Today was probably the funniest day for me. I made some tea with some of the fresh fruits and checked in on cake #6 throughout the day. Every time I came back I just kept laughing at this one fruit.20170308_090740.jpg 20170308_124752.jpg It continually pushed the cake closer and closer to the side of the quart jar. Finally the veil broke around 3pm and that's where I am in this grow20170308_161955.jpg 20170308_162210.jpg . I harvested the fruits on cake #7 and now  I'm seeing pins for its second flush. Cake # 4 is still on flush 2 and has pins coming from the bottom. 20170308_124802.jpg

 

I've loved every day of this experience and I look froward to many more. If anyone has advice or constructive criticism I'm all ears and ready to learn. 

 

 

 

 

 

 

 

 

 

 

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#2 tailsmcsnails

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Posted 09 March 2017 - 02:07 AM

I loved reading this!  How was the tea?



#3 MLBjammer

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Posted 09 March 2017 - 04:39 AM

That's some detail there.  Thanks for sharing.  We love this kind of stuff.


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#4 Hash_Man

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Posted 09 March 2017 - 08:17 AM

Yes great detail thanks. Only criticism might be is cakes like 95-100% humidity
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#5 Triptomemes

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Posted 09 March 2017 - 08:48 AM

I loved reading this!  How was the tea?

The tea was stronger than expected. I was testing it with a micro/sub threshold dose of .25-.35. It ended up feeling like a mellow 1.5g. I think this will be my main way of journeying from now on. 


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#6 archersmark

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Posted 09 March 2017 - 10:51 AM

very impressive documentation and notes.  I've learned more the better notes I take.  Also - the two things that helped me evolve as a grower were (1) Topia and the solid, accurate advice I've gotten here since I joined in Dec/Jan and (2) having a good fast growing strain which in my case was a Corumba Brazil


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#7 Triptomemes

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Posted 11 March 2017 - 08:58 AM

Woke up to cake #4 creating steps to the top of the cake. I'm curious to know if they grow faster overnight? or because I don't have my eye on them it just seems that way?  I'm hooked on the DEC now, it seems to produce more fruits IMO.20170311_0742281.jpg


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#8 MLBjammer

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Posted 12 March 2017 - 02:55 AM

Cubes can grow quickly.  In 24 hours they can often double in size.

 

Yes, a DEC will always make a difference.

 

Man, that is a pretty cake!


Edited by MLBjammer, 12 March 2017 - 02:55 AM.

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#9 fahtster

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Posted 14 March 2017 - 03:00 AM

Woke up to cake #4 creating steps to the top of the cake. I'm curious to know if they grow faster overnight? or because I don't have my eye on them it just seems that way?  I'm hooked on the DEC now, it seems to produce more fruits IMO.attachicon.gif20170311_0742281.jpg


Yeah, that's a photogenic cake for sure! :)

Faht

#10 Triptomemes

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Posted 15 March 2017 - 10:01 AM

UPDATE - I might be growing the first living Goomba  :biggrin:   :tongue:  goomba.jpeg  goomba art.jpg

 

Happy Wednesday Everyone!


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#11 MLBjammer

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Posted 16 March 2017 - 03:48 AM

I think it's a mutant, lol.



#12 orangutan

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Posted 17 March 2017 - 12:17 AM

nice work..  Looks very clean and on point.  This is only your first grow!

 

I didn't really start taking notes till recently.  It's amazing how much more one can learn from reading notes and comparing past grows to the current one.  You always think you will remember everything but you don't.

 

Keep it up.


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#13 spunforfun

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Posted 17 March 2017 - 12:32 AM

Nice write up and congrats on your first grow success!
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#14 Triptomemes

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Posted 17 March 2017 - 08:41 PM

Thanks spun and Orangutan.  I'm extremely grateful for this community; Even though I forage with a wild mushroom hunting group, I don't have the courage/trust to tell them I'm growing. But here I feel safe to express what's going on and you all give the best feedback.

I've started 1 more batch of BRF cakes (A1-A5) 20170315_0834511.jpg using the Honey LC.  I hope to move on to a dub tub after this batch of cakes is over. I'm still reading and trying to understand substrate/spawn ratios, as well as which grains to choose between popcorn and rye berries. 

 

Today I harvested:

cake #120170317_1934571.jpg 20170317_1938581.jpg  

cake # 220170317_1040101.jpg 20170317_1048371.jpg

cake # 320170316_1357161.jpg 20170316_1401391.jpg

cake # 520170317_1924371.jpg 20170317_1931521.jpg

 

Cake #2 I did a little experiment by setting it on a moist paper towel and verm on the top of the cake. Even though the flush was larger than any of my other cakes, I don't think I'll do that again because I also found 2-4 rotting pins on the bottom that had been over-saturated.

 

I'm drying them and planning on making a 3.5g Tea for a Sunday morning journey in my tent. 


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