Hello Mycofamily, the last few months have been very exciting for me. I finally joined this site, then went full force into the PF tek. I was really nervous about sterile technique and growing in general but I'm SO glad I gave this a try. This has to be one of the most rewarding, and humbling experience/hobby I've ever done. I only had to post one question throughout the entire grow, because everything I wanted to know had already been asked and answered in incredible detail. Thank you so much Mycotopia, I look forward to growing with this community.
Now I love reading the grow stories of others, so I decided from the start to keep a journal and pictures of each stage. I'm going to do my best to recapture the fun I've had over the last 2 months. I hope you enjoy it as well.
They are placed in a cabinet above the fridge to incubate at 73-75F
I was very determined to stretch my syringe through a LC so I used 2cc in a Honey LC(300ml) and 2cc in a Karo LC(300ml). They are being incubated in my closet at 77-81F
I had 1cc left in the Brazil MS syringe so I noced up one more jar(#6). I wanted to try out an airport lid with a vermiculite contamination layer just to see how it performed. I figured from the start that it would colonize slowly because of 1 inoculation point so I swirled the syringe as I injected the spore solution. I placed this jar in my closet to compare the effect of Temperature on colonization time.The closet was 77-81F
Jars #1-5 are doing well showing increased growth. Jar #4 (middle jar) has shown the most progress.( I should have put them in order but just for reference the jars are in order from left to right as #1,2,4,3,5)
I wanted to test out the LC so I used a tall 1/2 pint jar with 5 holes( 4 around the rim and 1 in the middle) and 1" of dry verm contamination filter. I injected 2cc into this jar (#7) It was also placed in the closet.
Jars #6 and #7 are coming along nicely. #6 has expanded its hold on the substrate #7 is showing growth at all the innoculation points . I wonder If the 5th hole in the center will help it reach 100% colonization faster.
I have to leave for a few days so this will be last check on the jars until the 15th.
Jars #1-5 are fully colonized and now will sit for a week of consolidation before moving to my SGFC. Jar # 6 is at 80% with a few places left uncolonized. I believe jar #7 is at 100% The lesson learned here is to never use only 1 inoculation hole. After reading MLBjammers Tek on in vitro quart jars I've decided to fruit #6 and 7 in vitro.
Jars #1-5 have been dunked and placed in the fridge. I have pins growing from cake #4 All the cakes smelled fresh and earthy.
Today was "interesting". I started by placing cake #6 into a quart ziplock jars with moist verm under it. Then by 1:21pm I was having issues with my RH staying near 88-90% in the SGFC, so I added another inch of perlite and re hydrated it. This went incredibly wrong when I placed the FC back in position only to watch about two cups of water leak out from the holes. I thought I had drained the perlite well( Clearly not). Sooo I ended up getting another sterlite container and rebuilding my chamber all evening. I dunked cakes # 1,2,3, and 5 so they wouldn't dry out and placed cake #4 in a ziplock quart jar. The other cakes dunked for 6 hours and then were moved into the new SGFC with 5inches of perlite;a RH of 95-97% and Temp of 68-74F. At 10:15pm I harvested my first mushrooms from cake #4 This made my whole day and got me even more excited for the rest of the cakes.
I DEC jar #6 to help with water retention. I covered up this little guy on the top . I hope he will be ok under the wet verm. I've also dunked and DEC jar #4 and placed it into a quart ziplock jar for invitro growing. The new SGFC is doing much better with RH of 97-99% and Temps are 70-75F. The cakes in this fruiting chamber are # 1,2,3 ,5 and 7. Cake #7 is the only one showing pins at this time.
Around 1pm I look at cake #6 and see all this fuzzy/hairy white stuff at the base of the fruits . So in a panic I quarantine it to another room and start asking questions on the site. In my impatience I assumed it was cobweb and treated the fuzzy feet with H202. Now I understand that this is completely normal mycelium formed from lack of FAE. I then took a 1/8th drill bit and added two more holes to the ziplock jar lid. I find that 5-6 holes is working better along with running the ceiling fan 3-4 times a day to move the air around.
The veil broke on this chubby guy from cake #6 but I didn't want to remove the cluster so I just removed the cap and left the immature mushrooms to grow. This would of been fine but 3 hours later there was a very sour smell coming from the jars so I removed the whole cluster . At the end of the day I've harvested 21.8g from two cakes.
I've moved all the cakes out of the SGFC today. I realized that Its not the best choice for me right now and I don't have the space or room to support it. Instead all the cakes are placed into individual quart ziplock jars. Cakes #1,2,3,and 5 still have no pins. Hopefully I'll have better results with individual humidities and FAE. Cake #4 was dunked and DEC again and now I have this odd growth coming off the side . I'm sure its a mushroom because I see gills but Its so small I'm not sure what to think. I'll just continue to watch it grow and take pics.
Today was probably the funniest day for me. I made some tea with some of the fresh fruits and checked in on cake #6 throughout the day. Every time I came back I just kept laughing at this one fruit. It continually pushed the cake closer and closer to the side of the quart jar. Finally the veil broke around 3pm and that's where I am in this grow . I harvested the fruits on cake #7 and now I'm seeing pins for its second flush. Cake # 4 is still on flush 2 and has pins coming from the bottom.
I've loved every day of this experience and I look froward to many more. If anyone has advice or constructive criticism I'm all ears and ready to learn.