Intro time: My name is Greddy. Im a black male with honey eyes and I enjoy cat naps, cat nips, and playing with the mouse on the computer.
Ive been out of the myco world for a few years and I am just getting settled into my new home and thought I would start back up with this hobby.
I ordered 1 b+ spore syringe and 2 amazonian spore syringes from a trusted source and noc'd up 6 wbs jars from each strains syringe after making some karo lcs. I took the second amazonian spore syringe and noc'd up 12 wbs qts.
I had ZERO success with the B+ the jars showed no growth at all. The LC showed no clouding despite being able to see visible spores floating in the water.
I had 3 jars of the 12 amazonian colonize normally and while the other 9 slowly battled with bacteria and were thrown out. The remaining 3 clean amazonian will further be referred to as "a237"
After the failed attempts with the first two syringes I buckled down a bit. I completely started from scratch as if it was my first day in the hobby.
Lc was redone in a new liquid culture specific jar with a new liquid culture specific lid with SFD and ship. Care was taken to ensure the lid was etched and the silicon would NOT come off without serious work.
I redid my wbs following a new tek and being incredibly cautious to water content and burst grain. My wbs was finally up to par. Dry to the touch, moist on the inside, plump, and burst kernels under 5%.
I shot 4cc of the remaining amazonian syringe into a fresh Karo Lc and 8 days later had a great looking cloud of myc. I took the LC and ran 4 test jars. "C1-C4" I ended up with 4 fully colonized beautiful jars 6 days later. Being limited in my quest to retain good CLEAN spores/lc, I began to research slurries and proceeded to slurry the qt of "C4" wbs. I used this to noc up 20 jars with approx. 1 tbsp of slurry. I used the remaining slurry to directly spawn a monotub of coir following the bucket tek. All the jars colonized very very quickly with only one showing a what appeared to by trich. Contam jar was removed before sporulation took place. Monotub spawned directly with slurry colonized very quickly but did develop trich before fruiting and was thrown out. At this point I decided to use up the rest of the colonized wbs jars I had laying around and save my remaining 6cc of spore syringe until I can get things figured out.
The wbs jars from the possibly tanted slurried "c4" jar will be referred to as "c4s2" I used these "c4s2" jars to do a few monotubs....
This tub was a C4S2 qt that was direct slurried to a monotub of coir/verm/and a 1 qt of black kow oven tek pasteurized for 6 hours. It was mixed and had a 1/8th inch casing layer put on top. This grew through extremely quick with extreme aerial mycelium. I thought this was due to lack of fae so I moved it into a large storage tote being fed by a coolmist, all of this inside my fruiting room which stays at 72-75F and approximately 50% RH. After moving to large coolmist fed tote aerial mycelium stopped and a non dense fuzzy mycelium developed. It did not appear to be cobweb to me, but I have been out of this for a while so maybe Im wrong. Its been sitting essentially unchanged for 3 days now.
The second tub was a coir bucket tek followed to the T with 5 qts of c4s2 spawn. Again this tub grew through the 1/8 casing quickly with a ton of aerial myc and at approximately 30% colonized casing it was placed into fruiting conditions. Only this time instead of slowing down it in three days grew over the whole casing. Surface is whispy and light not like bacteria though... this is close up, what do you guys think?
This third tub was done exactly as the second tub however this tub did not get nearly as much aerial mycelium and when place into fruiting conditions at 30% it sort of just halted. Looks sort of like a bacterial contam in the next close up picture. What do you guys think
I have some mini monotubs that I will be posting up when I return home that are exhibiting the exact same qualities despite different spawn from a different syringe entirely (a237) So now Im really starting to question what I have going on here as it does not appear to be genetic. When I was growing before I never had any of these aerial mycelium issues. Please advise great myco ones.