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Pans in Liquid Culture


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#1 phlegmbae

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Posted 19 March 2017 - 12:38 PM

I've been wondering if I do an LC of Pans if I should shake it? I found out the hard way that you shouldn't shake them in grain jars. I just don't want to make the same mistake again. Would horse poo broth leftover from pasteurizing work good alone, or should there be a tablespoon of karo added? Could I just soak horse poo for a few days, strain it out, and use that for Pan LC?



#2 peacefrog

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Posted 19 March 2017 - 07:31 PM

Pans should be treated exactly the same way you would any species of mushrooms. Shaking/distribution of grain is not an issue and is highly recommend for all species to aid in distribution of mycelium, allowing the spawn to colonize faster.

Why would you think you messed up by shaking grain spawn? What happened in your instance? The shake does not cause contamination, it exposes it if already present and unseen. That's why myself, and a lot of growers advise shaking up one last time before spawning, just to make sure.

As far as straight manure water as an LC, I couldn't answer that question. I have never tried it. But you could always experiment with it. I always use poo water in my agar for pans, so it just might work.

Edited by peacefrog, 19 March 2017 - 07:37 PM.

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#3 phlegmbae

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Posted 24 March 2017 - 12:47 PM

Thanks for your reply peacefrog. I highly value your opinion. I guess I failed to mention that when I shook my pans on rye berries, it never came back. Well at least not for a very long time, like a month. By that time I figured there was nothing in there anymore. It could have been a little dry from over processing, was my guess. So I was concerned that shaking a pan LC may do the same thing. I also failed to mention that to the horse poo water I will be adding Karo/honey to the broth.


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#4 peacefrog

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Posted 26 March 2017 - 06:43 PM

Gotcha, yea if after you shook up the grain jar and it didn't recover, you had contamination for sure. Wise to toss. Pans are very fast to colonize and if they haven't completely recovered in 24-48 hours after shaking, you know contams are present.

And I bet that any sugar like honey and/or karo in combination with poo water would work fine. As I stated, I always use manure juice in my agar jars with pans. Give it a shot, I would be quite interested in your results.

#5 Shiviah

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Posted 24 April 2017 - 10:39 AM

Just felt like adding a note here, but i do believe there are a couple species you do not want to shake grains for. I've heard that the Gymnopilus species do not recover well from shaking and if you're going for sclerotia on mexicana species shaking can often disrupt any formation of stones. In the latter species, p. mexicana, i've read it's best to innoculate with LC to prevent having to shake. 






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