I know there has been talk of this before, but I cant seem to find if anyone has actually done this yet. The idea is to add DMT to the mycellium, either in jars during colonization, or in the spawning medium, or both, to increase the production/concentration of DMT derivitives. Primarily, to increase production of 4HO-DMT and it's esters. The DMT would be added as one of the water soluble salts and thus would be freely available to the mycellium for conversion.
According to what I could find, the mechanism of conversion from Tryptamine (T) to and active (4PO-DMT, 4HO-DMT, or the NMT analogs thereof) is still unknown, but it has been found that elevated levels of T reduces the phosphate esters (4PO-DMT, 4PO-NMT, and 4PO-T) produced but increases the levels of psilocin (4HO-DMT). Both DMT and T have this effect.
So, since the alkaloid content is somewhere about less than 1% normally, and I have read about a psilocin content upwards of 3% with added tryptamine to the substrate. I wonder if adding DMT might increase this level further still.
From the paper:
"A dried cow dung/rice-grain mixture (2:1) with twice the amount of water was used to obtain fast fructifications without casing of a strain (3) of P. Cubensis. A 25 mM concentration of tryptamine (as hydrochloride) was added to this medium."
25mM of Tryptamine HCl is equivalent to: 4.9g/L
25mM of DMT Fumarate is equivalent to: 12.4g/L or would need about 9 grams freebase dmt to make into whatever salt of your choice.
That is an awful lot of DMT to be using, so I'm not so sure anyone has tried this before. I guess it really depends on which of the following is the new rate limited factor for producing psilocin:
If the methylation is the more difficult part for the fungus to achieve, then the levels of psilocin should be well above 3%.
Noone really seems sure exactly how all of this works in order of preference or in order of limiting enzymatic action:
Any thoughts on this?
Edited by BioTron, 02 April 2017 - 06:40 PM.