I translated a couple of Gartz' patents from German awhile ago, it's not perfect but all the important stuff is correct. There was an article in the Entheogen Review years ago, and a guy named Yachaj said the following quote. And it has been repeated a lot since, I will put the patent up soon but nowhere in the patent does it say brown rice flour. It clearly says grains of rice, there is no specification at all to color, variety or anything like that.;
Pollock soon concentrated on cheap and commonly available brown rice grains. He found that brown rice medium was highly selective for P. cubensis (no other mushroom could be fruited on it). Indeed, the selective quality of brown rice protects newbie psilonauts against accidentally growing toxic mushrooms. But there are more reasons to think of brown rice as superb. In the 1980s, German mycologist Dr. Jochen Gartz, went so far as to file a patent (No. 88-09773, Akad.Wiss. DDR) on brown rice after his discovery that this medium supported the cultivation of P. cubensis of unprecedented potency—1% psilocybin/psilocin by dry weight (which almost equals Panaeolus cyanescens), the highest natural potency ever reported of this mushroom.
From 'The Entheogen Review' vol X. no 4. winter solstice 2001. page 127
Gartz, Jochen, Dr.rer.nat.,DD
Process for obtaining tryptamine derivatives by cultivating higher fungi
The invention relates to a process for obtaining tryptamine derivatives psilocybin and psilocin by cultivating the fruiting bodies of higher fungi. The active substances are applicable as model substances in neurobiological research. The fruiting bodies are acheived after inoculation of an autoclaved mixture of rice grains and water with the primary mycelial culture after a few weeks, without further operational steps such as additional preparation of the substrate, use of a special
casing or constantly maintaining the humidity. The recovery of the active ingredients from the dried and powdered fruiting bodies by known methods such as extraction and chromatography.
A process for obtaining tryptamine derivatives by cultivating fruitung bodies of psilocybin and psilocin containing higher fungi, preferably of the genera Psilocybe, Strokaria, Plutens and Conocybe, distinguished by the fact that moist rice grains are used as a solid culture media, innoculated with primary cultures under sterile conditions and after the fruit body formation, the separation of psilocybin and prilocin known per se, as done by extraction or chromatography.
The method according to claim 1, is characterized in that after the growth of the mycelium through the culture media at a temperature of 20-25 * C, the cultivation of Fruit body takes place unsterilized, and depending on the fungus with or without additional exposure.
Culture media for cultivation of higher fungi, which can be used for the production of tryptamine derivatives, characterized by the composition
- Rice Grains 20-70%
- Water 30-80%
the mixture in an autoclaved state.
Field of application of the invention
The invention relates to a process for obtaining tryptamine derivatives by artificial cultivation of higher fungi on suitable nutrient media. The substances psilocybin and Psilocin are applicable as model substances in neurobiological research.
Characterisation of the known technical solutions
The tryptamines psilocybin and psilocin are found naturally occurring in higher fungi and are detectable in different species of the genera Psilocybe, Stropkaria, Plutens, Conocybe and Inocybe.
It is known that the fruiting bodies of various types of fungi of the genera Psilocybe and Stropkaria are artificially cultivated on compost substrates(DE 1087321).
From this the active ingredients can be prepared in their crystal form by means of suitable extraction methods and subsequent chromatographic separation. The method described has several disadvantages. An additional and lengthy process step is the preparation of the corresponding compost substrates. Furthermore, a casing layer for fructification is necessary, fruit body formation often begins only after many weeks. Finally, to achieve fructification, constant humidity levels of the air has to be ensured.
Furthermore, a substrate with rye is described as a main component for fruit body generation (J.Ethnopharm.5  287). the disadvantage of this method is likewise, the necessary application of a complicated composite casing layer with specific water-binding capacity while ensuring constant fertilization.
Object of the invention
The aim of the invention is to develop a simple and inexpensive process for obtaining tryptamine derivatives by culturing fruiting bodies of higher fungi,
in which a high concentration of tryptamine derivatives in the fungal tissue is achieved with simultaneous rapid fructification.
Explanation of the nature of the invention
The inventions object is to select such culture medium for fructification, which ensure a rapid fruit body formation at the same time high concentration of tryptamine derivatives in the mushroom without a casing and the need for constant humidification.
According to the invention the object is achieved by using a mixture of rice grains and water as a solid medium for fruit body formation. After autoclaving the mixture is sterile and the medium is innoculated at room temperature(20 to 25*C) with suitable primary cultures from agar plates. For the nutrient medium, 20 to 70% rice grains and supplemental amounts of water are used to get to 100%. After 3 to 4 weeks growth of the culture with or without additional exposure, per night mushroom species, the first fruiting bodies appear without use of a casing layer or necessary moistening, fruit will be harvested in the next few weeks, depending on the particular fructification wave. After the complete growth of the medium with mycelium, the fruit body formation can be continued even under unsterile conditions. In this procedure, psilocybin and psilocin are in quantities up to 1% and 0.5%, respectively, calculated on the dry masses of the fruiting bodies. The active compounds are prepared in a known manner by extraction with methanol and the following isolated with chromatography.
The invention is explained in more detail by the following examples:
A primary culture of Psilocybe cubensis (Earle)Singer was used for inoculation which was obtained by germination of the spores on 100ml of 6% malt agar, 14 days after innoculation and subsequent cultivation (20 days) on 100ml of the same agar.
A piece of mycelium about 2 x 2cm was removed from it with a sterile spatula and by shaking with 30ml of sterile water and 15g saddle packing, generated a suspension of fine mycelium flakes.
10ml of the suspension was used to inoculate an autoclaved mixture of 100g rice grains and 200ml of water. After 3 weeks the formation of the fruiting bodies began, which in 5 fructification waves appeared.
Yield: 21g dry fruiting bodies
HPLC analysis: Psilocybin content: 0.7%
Psilocin content: 0.1%
Workup is carried out in a known manner by extraction of the powdered material with methanol and subsequent column chromatography on cellulose.
A second strain of Psilocybe cubensis (Earle)Singer was used. Under analogous conditions, the first fruiting bodies were reached after 4 weeks.
Yield: 25g dry fruiting bodies
HPLC: Psilocybin-content: 0.95%