That's a red flag right there.
The hot water, and steam has probably killed off anything malicious living in there.
Of course one should try things and experiment, however it's not like you're the first member that has tried to skip "unnecessary work" or to try to pull off grows without proper pasteurization or sterilization (whichever is applicable). The bigger trap there is that if you are successful IMHO. I mean I could probably pull off a successful bulk grow skipping all sorts of steps and many growers have and will, however I'm trying to help set you up for success, and ignoring the empirical data and experience of the many growers that came before us is unwise.
On a more personal note: I believe in trying your own stuff, seeing what does and doesn't work so you feel confident that you're not doing unnecessary work. Should this box fail miserably, then I'll feel a lot better about the time I have to spend on my pasteurization.
Fair point. It was an educated guess though:
I was using a very small amount of substrate (about 1L) which allowed me to mix it properly right away.
Now, to the science:
As you can see, the temperature of the mixture (if properly mixed!) should be 72C after adding the boiling water. Google then told me that at 72C pasteurization takes just 15s while at 63C it takes 30min. I could then also calculate the heat loss from evaporation etc to see if that is maintained for long enough but decided to just add the hot water in the other bucket to delay the cooling. It was then left for more than long enough to fullfill the lower limit of 63C-30m.
For people reading this though: This is a very specific case. Normally you won't be able to properly mix the sub which would lead to certain areas possibly not being treated enough. Don't use a calculation like this if you can't ensure proper heat distribution!
Pasteurization is a process utilized to kill mesophillic microbes (in our case for mushroom substrates, not milk ). Bacteria (or their endospores) covers virtually every surface on earth, but the bacteria that we want to kill is the kind that thrive at the same temperatures that we and the desired mycelium do. Don't forget mold spores! Improperly treated substrates can harbor mold spores that are parasitic and can destroy an entire grow and compromise that space for a long time.
A couple extra hours at the proper temperatures using flawless technique is so much easier to do than the amount of cleaning that one has to employ after waking up to mold spores covering a substrate. Once it sporulates, yer fucked. That means that a parasitic mold that favors eating mycelium has just covered the entire area with billions of spores, just waiting to germinate on it's next 'victim'.
Thanks for the info! I think I should get a book about the background of it to better understand the microbial stuff. I'm a mechanical engineer so biology is not really my forte.
Might be fun to try this:
Volumetric Air Sampling For Detecting Viable Airborne Mould Spores And Fragments
The settle plate method is generally not recommended. A better method involves impacting a known amount of air on some suitable growth media. The media could be liquid or solid. With this method viable spores or vegetative material would form visible colonies (referred to colony forming units) in the media. These are counted in the lab and expressed as colony forming units (CFU) per cubic meter of air.
When I get my agar set up to see what my CFU/m3 is in my room.
I was lying in bed yesterday thinking about the bags I'm going to be sterilizing (shrooms seem to be the only thing on my mind as of late ). I planned to use the tyvek sleeve tek, basically tyvek in the top of the bag folded like an accordeon. An idea popped into my head though:
Would it be possible to take an air-port syringe (with some polyfill in there), stick it through the microfilter, tape it with some gentle tape and pressure cook with it in there? The syringe would allow clean air exchange during the sterilization and afterwards I would just remove it. That should give me perfect sterilization and no chance of contaminating it while trying to close the bag. Any thoughts? I'll give it a whirl when my finals are done but I would like to hear what you guys think I tried it out with a regular plastic bag and it let through enough air that I couldn't pop the bag so that's one part?
Anyone have any thoughts on this?
Edit: Almost forgot the jar update:
Edited by MrModeon, 11 April 2017 - 04:04 AM.