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CatsAndBats attacks some prints (easy aseptic printing)


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#21 jkdeth

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Posted 17 April 2017 - 11:41 PM

Almost VIP. Paid and awaiting permissions.
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#22 jkdeth

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Posted 18 April 2017 - 01:10 AM

All paid up and VIP
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#23 SteampunkScientist

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Posted 18 April 2017 - 05:12 AM

Cat....

I meant the bunny​.... ;-)

Edited by SteampunkScientist, 18 April 2017 - 05:13 AM.

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#24 MLBjammer

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Posted 18 April 2017 - 05:27 AM

Nice write-up, Cat.  Glad to see you are still playing around with Redboy.


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#25 CatsAndBats

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Posted 18 April 2017 - 07:13 AM

Cat....

I meant the bunny​.... ;-)

 

 

That'll be on "catsAndBats attacks agar 4.0" I'm just waiting for the right tissue sample!

 

 

d99fe3652c2f7199c53f4a2459f57863.jpg


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#26 CatsAndBats

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Posted 18 April 2017 - 07:44 AM

All paid up and VIP

 

 

Hit me up in mycochat. :biggrin:



#27 jkdeth

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Posted 18 April 2017 - 07:47 AM

Will do, still waiting for the chat invite.
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#28 CatsAndBats

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Posted 18 April 2017 - 07:50 AM

Will do, still waiting for the chat invite.

 

Check your spam box too if it doesn't show in the next couple days.



#29 peacefrog

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Posted 19 April 2017 - 05:36 PM

Nice, brother. Vault material for sure!
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#30 CatsAndBats

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Posted 19 April 2017 - 06:03 PM

Nice, brother. Vault material for sure!

 

 

Thank you sir, it means a lot coming from you.


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#31 peacefrog

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Posted 19 April 2017 - 06:14 PM

You deserve it, man. You are on here helping and advising growers more than anybody, especially with posts like this! Topia wouldn't be the same without you, that's for sure.
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#32 425nm

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Posted 22 April 2017 - 02:28 PM

Nice write up Cat.
My only quibble for those newer to printing would be to specifically recommend 70% isopropyl alcohol rather than 90% (though you don't specify what kind of alcohol). 90% will certainly work but it's less effective than 70%. See:

http://microbiologys...-as_21.html?m=1

You might not see much of a difference since you're aseptic technique is so polished but it could make a difference for those less practiced.
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#33 CatsAndBats

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Posted 22 April 2017 - 04:23 PM

Nice write up Cat.
My only quibble for those newer to printing would be to specifically recommend 70% isopropyl alcohol rather than 90% (though you don't specify what kind of alcohol). 90% will certainly work but it's less effective than 70%. See:

http://microbiologys...-as_21.html?m=1

You might not see much of a difference since you're aseptic technique is so polished but it could make a difference for those less practiced.

Honestly, my mix in my bottle is prolly at 80% cause I get stoned and talk myself into 70% is better one time and 90% is better this time and so on..

 

Good looking out.



#34 Heirloom

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Posted 26 April 2017 - 03:51 PM

After the spores drop, in your clean area, pull the spent caps out, replace the covers and let them dry for a day or so in order to make sure that they don't germinate early.



Cat mentioned making sure the prints has dried for a day or so, I am going to follow that advise.


This is what happened when I didn't let the print dry enough, this print germanated, looked dry when I bagged it.

hey Cat, I am going to put this into a syringe today, any reason not to ?


Great thread.

Attached Thumbnails

  • AS 001.JPG

Edited by Heirloom Spores, 26 April 2017 - 03:54 PM.

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#35 CatsAndBats

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Posted 26 April 2017 - 04:05 PM

 

After the spores drop, in your clean area, pull the spent caps out, replace the covers and let them dry for a day or so in order to make sure that they don't germinate early.



Cat mentioned making sure the prints has dried for a day or so, I am going to follow that advise.


This is what happened when I didn't let the print dry enough, this print germanated, looked dry when I bagged it.

hey Cat, I am going to put this into a syringe today, any reason not to ?


Great thread.

 

 

 

Aside from I'm in the 'germinate to agar' school of thought, why not? I find that being mindful of ambient RH% is helpful in determining how fast to bag a print or how long to leave fruits on the dehydrator etc..


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#36 Heirloom

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Posted 26 April 2017 - 04:08 PM

Thanks
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#37 FieldTrip

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Posted 04 May 2017 - 11:28 AM

I've got a nice fat fruit I'm going to make a print with this morning. Will be following cats instructions to the T. Btw cat, are those malabar prints ready??

#38 CatsAndBats

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Posted 04 May 2017 - 11:36 AM

are those malabar prints ready??

 

I'm on mushroom time. It's finally a low enough rh paired with I the have time to do it.

 

I've got a nice fat fruit I'm going to make a print with this morning. Will be following cats instructions to the T.

 

only one?



#39 FieldTrip

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Posted 04 May 2017 - 11:55 AM

are those malabar prints ready??


I'm on mushroom time. It's finally a low enough rh paired with I the have time to do it.

I've got a nice fat fruit I'm going to make a print with this morning. Will be following cats instructions to the T.


only one?
Touché. Yea, there's only one that is dying to be printed atm. I've got my eye on a couple others, they just aren't big enough yet.

Sent from my LG-H910 using Tapatalk
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#40 CatsAndBats

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Posted 04 May 2017 - 11:59 AM

 

 

are those malabar prints ready??


I'm on mushroom time. It's finally a low enough rh paired with I the have time to do it.

I've got a nice fat fruit I'm going to make a print with this morning. Will be following cats instructions to the T.


only one?
Touché. Yea, there's only one that is dying to be printed atm. I've got my eye on a couple others, they just aren't big enough yet.

Sent from my LG-H910 using Tapatalk

 

 

that's what she said






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