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Ferather's Corner | Experiments, tests and data [Magic edition]


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#1 Ferather

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Posted 12 July 2017 - 03:09 PM

Decided to pump all of my experiments and data related to magic rather than gourmet mycelium here, rather than the gourmet section.

 

 

Currently I am attempting to germinate Psilocybe cubensis "Burma" spores onto a pH modified black tea bag.

The reason is the tea bags contain all the nutrients I need, and only trace sugars no starch.

 

Nutritional composition, external links: Tea leaves, Brown rice, Wheat bran.

 

If it works I will be using it to spawn to WL-Tek for testing.

 


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#2 Ferather

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Posted 12 July 2017 - 03:42 PM

Here is my previous attempt with cyans, which grew out well, but needed pH modifying.

 

1.jpg 2.jpg 3.jpg 4.jpg


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#3 Arathu

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Posted 12 July 2017 - 06:01 PM

Looking forward to this............are you going to get some to fruit?  :rolleyes:  You NEED to eat some for testing...........hahahahaha

 

I love tripping with intellectuals..................if I had the cash I'd fly across the pond............. and take you to Amsterdam..........

 

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#4 Ferather

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Posted 12 July 2017 - 06:13 PM

Fruiting is intended, once I am complete with cubensis I will be studying Psilocybe cyanescens then Psilocybe ovoideocystidiata.

I am starting with cubensis because of their fruiting temperature, and high CO2 tolerance, making it easier.


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#5 Arathu

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Posted 12 July 2017 - 06:50 PM

Excellent..............

 

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#6 Cue

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Posted 14 July 2017 - 05:33 AM

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#7 Ferather

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Posted 17 July 2017 - 10:48 AM

The cubensis spores have germinated, and started growing out, this proves cubensis is primary and does not need starch.

Additionally it likes a higher pH substrate, the same target pH as oysters and cyans (pH 7-8), eats wood too.

 

The black at the top of the peg is rust, I could not find my normal cutters.

 

IMG_20170717_161015.jpg


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#8 Ferather

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Posted 17 July 2017 - 10:49 AM

It took 4-5 days to germinate, pretty normal in germination speed.



#9 Ferather

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Posted 18 July 2017 - 02:47 PM

Well, interestingly its growing up and from the wood peg. The tea leaves contain 3% nitrogen (rich), whereas the wood contains about 0.2% nitrogen (weak).

Like oysters, its pushing out for more nitrogen, whereas the tea at 3% is slow to colonize (nitrogen heavy), I will be transferring to T-Gel soon.

 

Hopefully the cubensis will properly adapt, and produce cellulase and laccase to decay plant fiber materials, other than straw.

Current growth is weak, however it appears to be digesting both the wood peg and the tea leaves.

 

IMG_20170718_170232.jpg IMG_20170718_170355.jpg



#10 Ferather

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Posted 18 July 2017 - 04:34 PM

My inconclusive data so far:
 
--[A: Black tea bag, wood peg, pH 7 --[B: Organic rye grain, pH 7 --[C: WL-Tek, few drops of sugar water, pH 7.
 
Samples B and C eventually stalled to bad GE, so I need to reproduce B and C.
 
A: IMG_20170718_170355.jpg B: IMG_20170515_163635.jpg C: IMG_20170531_130448.jpg


#11 OysterFarmer

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Posted 16 September 2017 - 03:48 PM

This is interesting.  I'm starting to work on something similar with cyans.  We should compare notes.


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#12 Ferather

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Posted 08 October 2017 - 03:59 PM

Based on recent tests with gelatin (protein), I will be attempting to germinate cyan's and cubensis on the same media.
I will not be adding sugar or starch, I will be using paper pellets, tea, soluble nutrients and 240B gelatin.
 
Calcium carbonate (CaCO3) will be used to balance the pH to required levels, as it's not toxic.
There is no known overdose limit with CaCO3, only the high pH is an issue, raw.
 
For the cyan's I will need to germinate at 10-18°C, the same as fruiting.
Once germinated, the cyan's will be colonized at 20-24°C.
 
 
Experimental recipe:
 
1g > MG, soluble nutrients.
75g > Boiling hot water.
25g > Paper pellets.
0.8g > 240B gelatin.
3g > Tea leaves.
4-6g > CaCO3.
 
pH 6.5 - 7.
 
----
 
Here is the cubensis, and cyan's (in a fridge), 08/10/2017 : 18:42 (UK).
For the cubensis I picked up some dry spores from a print.
 
1.jpg 2.jpg 3.jpg

Edited by Ferather, 08 October 2017 - 04:01 PM.


#13 Ferather

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Posted 09 October 2017 - 05:19 PM

I have a question, I managed to store a tissue sample for over 4 months in calcium bicarbonate water, essentially preventing oxidization (preserved).

Since the active ingredients, in actives, decay to oxygen, would carbonated water or even sparkling lemonade, coke etc work to store it?

 

If you picked lets say cubensis fresh, and blended it with lemonade, then stored in a bottle to be consumed later.

 

Hypothetical recipe:

 

> 20g fresh magic mushrooms.

> 480ml lemonade, other.

> 2 teaspoons of sugar.

 

Add 240ml lemonade to a blender, add the mushies and sugar, blend.

Add the other 240ml, add everything to a 500ml bottle, store.

 

The bottle will cut off the air, lemonade is carbonated.



#14 Jeepster

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Posted 09 October 2017 - 05:27 PM

You have my attention.

#15 Ferather

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Posted 09 October 2017 - 05:37 PM

Here is the post I made a while ago, post 15 is oysters oxidizing in the air, slow rate of decay due to low temperature.

The tissue sample was not fully sealed, and likely some oxygen dissolved into the water, best sealed.

 

The sample was used and grew out in 4-5 days, so over 4 months is expected @ 12°C.

Cutting off the O2, using fluids, and lower temperatures all play a role.



#16 Ferather

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Posted 09 October 2017 - 05:39 PM

CaCO3 (calcium carbonate) conclusion:
 
> Calcium bicarbonate water, prevented oxidization, and keeps tissue samples alive for long periods.
> CO2 expelled by mycelium, reacts with insoluble "CaCO3" to produce soluble "Ca(HCO3)2".
> Heavy hydration, sudden pH or carbon ratio changes, trigger pinning (may vary).
> I got a growth response from a 2 year old spent cake, full of waste.
 
Can be used to help remove waste from substrates.
 

Calcium oxalate, calcium-oxalate production.


Edited by Ferather, 09 October 2017 - 05:44 PM.


#17 Ferather

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Posted 12 October 2017 - 01:17 PM

Ok so 4 days now, and the cubensis test has so far no cubensis (expected), however it does have a random wood lover.
Whatever it is, germinated in 4 days using the tea and tea extracts, it appears to be growing pretty quick.
 
Basic conclusion so far: Adding tea, and extracts, turns the enriched paper into rich wood.
Due to wood loving spores in the air, this method requires aseptic working.
 
1.jpg 2.jpg


#18 Ferather

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Posted 12 October 2017 - 01:32 PM

Instead of exporting a ton of data from another forum, I will simply add the following:

 

Cubensis do not produce laccase, if they do it's very little, whereas species armed with laccase will germinate and grow out using lignin, and other similar materials.

These materials, are also known as plant extractives (water soluble), being soluble makes it "quick" and "easy" to utilize, lignin can cause contamination.

 

Additionally the absence of the tea and extractives, reduces contamination to, none seen for over 6 months, semi exposed, 100% cellulose.

Spores should not germinate on complex cellulose, even enriched, alone, "quick" carbon must be present to do so.

 

Cubensis germinates best on cellulose, when minimal levels of sugar or starch (both quick), are present.

Cellulose compatible bacteria, will generate solubles via digestion, can trigger germination.

 

1.jpg


Edited by Ferather, 12 October 2017 - 01:37 PM.


#19 Ferather

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Posted 12 October 2017 - 02:36 PM

Just did some reading, from here, and it looks like cubensis will produce tiny amounts of laccase on certain medias.

It should be possible to grow out and isolate growth on medias requiring the laccase enzyme.

 

Eventually you "might" end up with some very "special" genetics.

 

Edit: nvm wrong cubensis.


Edited by Ferather, 12 October 2017 - 03:18 PM.


#20 Ferather

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Posted 12 October 2017 - 02:44 PM

Cubensis primary enzyme list:

 

Proteins | Protease | Carbon and nitrogen source.
Cellulose, other | Cellulase | Carbon source.
Starch | Amylase | Carbon source.
 
Sugars, Carbon source.

Edited by Ferather, 12 October 2017 - 03:23 PM.





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