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Ferather's Corner | Experiments, tests and data [Magic edition]


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#61 Ferather

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Posted 13 May 2019 - 10:09 AM

12/05/19 - Azure sample to T-Gel:
 
Slower than Cubensis, although this may be due to various factors, such as pH. I have varied genetics, and will be isolating 9 o'clock.
The mycelium at 9 o'clock has produced an abundant amounts of cells, it's aerial, either for oxygen or lack of space.
 
IMG_20190512_133417.jpg     IMG_20190512_133632.jpg     IMG_20190512_133743.jpg     IMG_20190512_133902.jpg
 
====
 
13/05/19 - Azure sample to T-Gel:
 
It's still focusing on population, growth is spread and not rhizomorphic. You can generally see where rhizomorphs would have formed (gaps).
The decay and-or phenols appear to be reacting to torch light (LED 6500K), most notable as an outer hue in image 1.
 
The genetics in the 4th quarter (9-12 o'clock) appear to have produced the best overall growth.
 
IMG_20190513_152839.jpg     IMG_20190513_152925.jpg     IMG_20190513_152934.jpg     IMG_20190513_153221.jpg
 
It's seems messages where passed around, rather than genetics.
 
====
 

Edited by Ferather, 13 May 2019 - 01:50 PM.

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#62 Ferather

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Posted 15 May 2019 - 01:45 PM

Azure wood sample to T-Gel:
 
All of the growth has finally adapted, it's almost a brilliant white, no visible contamination. It's a pass, and I can transfer anywhere.
The only downside to using T-Gel is that it takes much longer, due to decay + conversion and lack of sugar.
 
IMG_20190515_192450.jpg     IMG_20190515_192554.jpg     IMG_20190515_192559.jpg     IMG_20190515_192604.jpg
 
Transfer 2 will have additional MG (Miracle-Gro), for testing.
 
MG.png

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#63 Ferather

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Posted 22 May 2019 - 08:59 AM

Azure wood sample to T-Gel (yesterday's images):
 
Visible multi-genetics, no doubt the spawn was made without any isolates (probably from spores). Some of the mycelium has fused, some have not.
In some areas you can see what would have been rhizomorphs, except they are not aggregated (not clumped, instead it's spread).
 
In addition, the total carbon content in phenols-other is much higher than ME per gram (3.2g leaf = 1.2g soluble).
 
IMG_20190521_160118.jpg     IMG_20190521_160126.jpg     IMG_20190521_160141.jpg     IMG_20190521_160156.jpg     IMG_20190521_160205.jpg
 
IMG_20190521_160415.jpg     IMG_20190521_160423.jpg     IMG_20190521_160429.jpg     IMG_20190521_160439.jpg     IMG_20190521_160450.jpg
 
Not yet a uniform colony, with possible multi-genetic competing.
 
====
 
T-Gel, in a nutshell:
 
Phenols-other require oxidation to decay, oxidizing agents also decay organisms (cell-other damage), organisms which produce antioxidants can survive.
T-Gel contains no sugar, starch or cellulose, only organisms capable of decaying and converting phenols-other can grow.
 
Cons: 25-33% slower then standard agar recipes, generally cannot be used to germinate spores.
 
Notes: Requires more oxygen than some current agar setups. 
 
----
 
WL-Tek, in a nutshell:
 
Does not add starch or sugar to the main media, reducing contamination chances largely in comparison to bran-other (contains starch, sugar).
The soluble phenols-other are removed during the paper making process, the only source would be any added materials (wood).
 
Cons: Starch based spawn must be used, as the complex cellulose is relatively non-reactive and slow to decay.
 
Notes: Various mycelium may prefer a different pH, and level of nutrients (example, less).
 
----
 
Feel free to ask me questions in relation to my T-Gel or WL-Tek.

Edited by Ferather, 22 May 2019 - 09:16 AM.

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#64 Ferather

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Posted 28 May 2019 - 01:31 PM

Just assembled my T-Gel for the 2nd transfer of the Azure I have on T-Gel, plain.
 
Recipe: 100g Water, 3.2g Black tea (bag), 2g Agar, 0.08g MG (see here).


#65 Ferather

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Posted 29 May 2019 - 06:44 AM

I have recovery with transfer 2 Azure, with added MG.
Roughly 18 hours, and 3 out of 4 sides.
 
IMG_20190529_113831.jpg


#66 Ferather

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Posted 29 May 2019 - 02:52 PM

Azure transfer 2, + 8 hours from before, notable growth.

 

IMG_20190529_203915.jpg   IMG_20190529_203957.jpg



#67 Ferather

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Posted 30 May 2019 - 05:22 AM

Here is today's Azure transfer 2, and also a Tarragon oyster colony flesh sample, regenerating (48 hours).
The Tarragon flesh sample was pulled from colonized wooden dowels (1.5 years old).
 
IMG_20190530_095845.jpg   IMG_20190530_100145.jpg


#68 Ferather

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Posted 30 May 2019 - 04:13 PM

< ~12 hours later, about twice as fast as T-Gel plain.
 
IMG_20190530_214916.jpg   IMG_20190530_214945.jpg
 
----
 
Flesh sample, 8 hours later (from above).
 
IMG_20190530_192011.jpg

Edited by Ferather, 30 May 2019 - 04:14 PM.


#69 Ferather

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Posted 03 June 2019 - 09:00 AM

I got an interesting reaction to added MG, it's producing more enzymes-reactants than previously, and also penetrative growth.
Still no rhizomorphs, however it's likely this type of growth would do well on solid wood, other medias.
 
Based on previous images (lack of black ring), growth was fueled by the wedge.
 
IMG_20190602_142620.jpg   IMG_20190602_143117.jpg
 
----
 
Adapted Cubensis did the same to start with, with added MG.
 
IMG_20190105_155156.jpg   IMG_20190115_215545.jpg
 
White-rot effect (bleaching).
 
====
 
Now the mycelium is receiving more carbon-other from decay, it visibly morphed overnight to a more normal appearance.
Population rate (amount of mycelium per area) has also increased, it's nearly (and soon to be) radial.
 
It would seem it started with more nitrogen than carbon other, which produced morphs.
 
IMG_20190603_131910.jpg   IMG_20190603_131956.jpg


#70 Ferather

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Posted 04 June 2019 - 02:35 PM

IMG_20190604_202002.jpg   IMG_20190604_202159.jpg



#71 Ferather

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Posted 25 June 2019 - 08:20 AM

Azure transfer 2, T-Gel + MG:
 
Turns out the little patch at the beginning was bacteria-other, the good news it did not colonize, it's cut off but slowly building upwards (still alive).
The added MG seems to have made the bacteria-other more visible, as before it was totally hidden and could not be seen.
 
The downside is, while it's cut off and I can make transfers, the mycelium is unable to destroy, remove it.
In addition, the bacteria-other, came from the vendor wood sample used in transfer 1.
 
IMG_20190625_124604.jpg   IMG_20190625_124653.jpg


#72 Ferather

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Posted 25 June 2019 - 01:08 PM

It turns out some nitrogen fixating bacteria can produce a slime on agar, and they can also decay wood (cellulose-other).
Makes me wonder if the added nitrogen (MG) altered the possible symbiotic relationship (not needed).
 
After careful inspection, I am unable to find any other locations with slime or patches.

Edited by Ferather, 25 June 2019 - 01:08 PM.


#73 Ferather

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Posted 27 June 2019 - 11:19 AM

Decided to run my Cubensis Burma tests. I am using 100% wood pellets, it's light pine (recycled, soaked then pelleted). So far these wood pellets are the best ones I've used, currently they are holding over 75% water.
I'm testing WL-Tek, general (with optional CaCO3, calcium carbonate), but instead of paper, 100% wood, and a wedge. Note, my Burma is adapted to decay T-Gel (phenols-other).
 
----
 
Well, here is some positive info. T-Gel plain contains no sugar, starch or cellulose, the main carbon source is phenols-other (not composed of glucose).
When I removed the top to take a wedge, the outer mycelium (closest to oxygen), begun bruising, so I took images.
 
IMG_20190627_163234.jpg   IMG_20190627_163321.jpg   IMG_20190627_163743.jpg





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