Typically when we see mycelium growing, it is very probable that is dikaryotic or already mated. By spore dilution i mean taking a piece of nicrome wire and touching a spore print then swirl it around in 100 ml or more of water then aspirate the water into a syringe then disperse single drops via 27 gauge sharp onto agar. Couldn't imagine how many drops that would be. Just talking about it makes me anxious about it allover again. Im trying to balance life and a new career at the moment but once i balance it, i will hit the mycology with intensity again.That is very interesting, I would love to hear where your research led.
Creating a double haploid or doubling the chromosomes in a single monokaryotic culture is something i was researching but i needed a break.Exactly, overcoming the mating types and achieving complete homozygosity would be a game changer!
If successful it would open the doors wide open for selective breeding including crossing or creating hybrids by being able to truly control the lineage.
I have noticed that a dilution onto agar followed by lowering the incubation temp, makes the germination play out a lot slower and you would have a bigger window to grab cultures as they germinate.
Edited by Microbe, 15 March 2019 - 07:30 AM.