The purpose of this step is very simple. You need to get the mycelia rich LC out of the jar and into a syringe so you can inoculate your substrate jars.
You will need:
Start by shaking up the LC jar thoroughly. Flame the airport syringe red hot, let it cool and inject it through one port of the LC jar. Put the needle on the empty syringe and inject it through another injection port. Draw back the plunger and fill the syringe with LC. Remove the syringe and cap it immediately.
- Colonized LC jar
- Sterile airport syringe with needle
- Sterile 10mL (or 60mL) syringe
- Sterile 16 gauge needle
- Mini blowtorch lighter
Removing liquid and replacing air.
If the LC has been allowed to get too thick, the needle may clog with mycelia. If this happens, squirt a little LC back into the jar, shake, and begin drawing back the plunger again. Very thick mycelia water may take five or six start/stops to fill the syringe.
A 60mL syringe filled with LC.
BB makes a mycelial tissue syringe from LC
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Posted 20 August 2006 - 12:46 PM