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mycogone perniciosa or paranoia? pleurotus eryngii on pf cakes


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#61 CatsAndBats

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Posted 24 January 2018 - 11:40 AM

post-147940-0-95382300-1516811746.jpg

 

Today.

 

post-147940-0-73333000-1516811749.jpg

 

As you can see, it's not lack of o2, as there are 3 1/2" holes on the face of the dubtub and 3 more on the opposite side, placed higher to force circulation. It's been in a window with southern sunlight, temps 60-70f. No filter over holes.

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#62 CatsAndBats

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Posted 25 January 2018 - 11:24 AM

Bump before spore drop



#63 Ferather

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Posted 25 January 2018 - 07:03 PM

Not true, it's still O2, the stems have stretched and the caps are small.

King oyster is practically the only oyster that can do low O2.

 

Medium O2 (no top + lots of slot vents):

 

IMG_20160729_212252.jpg

 

Maximum O2 (outdoors):

 

IMG_20160510_152615.jpg


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#64 Ferather

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Posted 25 January 2018 - 07:05 PM

Also your images here, have small or no caps, some have "fuzzy feet" which is due to low O2.

 

Hope that helps.


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#65 CatsAndBats

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Posted 25 January 2018 - 08:11 PM

Not true, it's still O2, the stems have stretched and the caps are small.

King oyster is practically the only oyster that can do low O2.

 

Medium O2 (no top + lots of slot vents):

 

attachicon.gifIMG_20160729_212252.jpg

 

Maximum O2 (outdoors):

 

attachicon.gifIMG_20160510_152615.jpg

 

 

 

Very interesting, I've only ever fruited oysters outdoors. I'd take the top off, but the fucking cats will prolly fuck them up. Any idea if that is a pleurotus strain, cuboid pointed out that it could be the one look alike?


Edited by CatsAndBats, 25 January 2018 - 08:11 PM.


#66 Ferather

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Posted 25 January 2018 - 08:39 PM

You will be surprised how many things a tub can "build-up" and how many things can "sink" to the bottom.

The reason you are using the tub is to "build-up" humidity, else why are you using it, hehe?

 

----

 

I am no expert identifier, however they do look like typical oysters, and certainly the indoor ones.

I see trumpet caps, and browning to light, it looks a lot like Pleurotus cornucopiae.

 

Pleurotus cornucopiae varies with substrate and environment.

 

----

 

Should really get it checked, so you don't die!


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#67 Ferather

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Posted 25 January 2018 - 08:41 PM

If you like, pop a sample on T-Gel plain, no additives, in case it has internal bacteria from the wild, happened to me.


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#68 Needles

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Posted 25 January 2018 - 09:33 PM

You will be surprised how many things a tub can "build-up" and how many things can "sink" to the bottom.
The reason you are using the tub is to "build-up" humidity, else why are you using it, hehe?
 
----
 
I am no expert identifier, however they do look like typical oysters, and certainly the indoor ones.
I see trumpet caps, and browning to light, it looks a lot like Pleurotus cornucopiae.
 
Pleurotus cornucopiae varies with substrate and environment.
 
----
 
Should really get it checked, so you don't die!


Your so right when saying how fast things build up. When talking about C02 levels building up it doesn't take long. Using a C02 meter to measure levels I found at around 2000 foot outside elevation that C02 levels were at 350ppm. indoor in my office, about 120 square foot was 675ppm. Within a hour of work with just my restoration brought the room up to 1200ppm. It just shows how fast a small area can accumulate high levels of C02.......
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#69 Ferather

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Posted 25 January 2018 - 09:51 PM

Indeed, mycelium expel CO2 and intake O2, like humans, except they are more micro, and can tolerate low conditions.

It's actually the level % of O2 that should be measured for grows, not CO2 as much, it's not an anaerobe.

 

I agree low O2 pushes out mycelium, but high O2 decays medias more and faster.


Edited by Ferather, 25 January 2018 - 09:52 PM.


#70 Ferather

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Posted 26 January 2018 - 07:44 AM

Most things are in my pocket guide in my journal, here (Mycotopia hosted file).

 

Starch is "in vitro" for lignicolous mycelium, which normally decay phenol's, cellulose and proteins. Cubensis does not have laccase, and does not target phenol's.

Additionally the phenol's which it cannot target, can be inhibitory, unless lots of starch-sugar is added and the pH corrected (leeching or CaCO3).

 

Due to no laccase, Cubensis requires less O2 to decay a substrate, but also requires a replacement carbon source.

 

----

 

Extract from my pocket guide, with added Amylase cleavage:
 
----
 
Enzymes
 
Disclaimer: Optimal pH varies with the organism, and it's enzymes.
 
 
Cellulase (cellulose): 4.5-8.5.
Laccase (phenol's): 5.5-6.0. < Lignicolous mycelium.
Amylase (starch): 3.0-7.0.
 
Protease (protein): 2.5-11.
 
 
For cellulase there are four types:
 
Alkaline cellulase: 7.2-8.5.
Neutral cellulase: 6.0-8.0.
Hybrid cellulase: 4.5-7.0.
Acid cellulase: 4.5-5.0.
 
 
For amylase there are three types:
 
α-Amylase: 6.7-7.0. < Any amount of glucose.
β-Amylase: 4.0-5.0. < Cleaves two units.
γ-Amylase: 3.0. < Cleaves one unit.
 
 
Laccase remains stable at pH 5.0-10.0.
 
 
For protease there are three types:
 
Alkaline protease: 7.0-11.0.
Neutral protease: 6.0-9.0.
Acid protease: 2.5-4.0.
 
----
 
Proteins count both as a carbon source and as a nitrogen source.
 
There are also other nitrogen sources other than protein.
Some nitrogen sources don't need enzymes at all.
 
Calcium bicarbonate also needs no enzymes.

Edited by Ferather, 26 January 2018 - 07:44 AM.

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