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Cubensis [Data Log]


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#21 Ferather

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Posted 10 November 2018 - 09:55 AM

Some further thoughts, as I was looking at the black oxidation ring (tea phenols turn black when they decay to oxygen).
I may need to increase GE (oxygen intake), more than normal (for example sugars), for improved decay.
 
I cannot conclude without a LAB, what types of laccase and-or peroxidases Cubensis uses.
 
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Next test: 100g Water, 2.4g Agar, 1.6g Black tea, 0.8g Sucrose, 0.04g MG.
 
Note: Around 25-33% of the tea is soluble, not all of it.
 
 
Nutrients:
 
 
 
Proteins, varies:  [ Cx | Hx | Ox | Nx ] x. -- Trace, minimal.
Polyphenols, varies:  [ C6 | H5 | O ] x. -- Phenols.
Cellulose: [ C6 | H10 | O5 ] x. -- Trace, debris.
 
Theobromine:  C7 | H8 | N4 | O2. -- Phenol.
Theophylline:  C7 | H8 | N4 | O2. -- Phenol.
Caffeine:  C8 | H10 | N4 | O2. -- Phenol.
Theaflavin:  C29 | H24 | O12. -- Phenol.
Tannins:  C76 | H52 | O46. -- Phenol.
Catechin:  C15 | H14 | O6. -- Phenol.
 
Carotene:  C40 | H56. -- Hydrocarbon.
 
 


#22 Ferather

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Posted 11 November 2018 - 09:17 AM

I transferred the peg yesterday (24 hours ago), I forgot to wipe the inside of the lid after sterilizing it.
Regardless, the Cubensis has recovered and started growing out (also on the agar).
 
The black oxidation ring has already appeared due to active enzymes.
 
IMG_20181111_124111.jpg IMG_20181111_124208.jpg IMG_20181111_124228.jpg
 
Now with more GE, more oxygen.


#23 Ferather

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Posted 12 November 2018 - 04:03 PM

[Recipe B + More GE]
 
48 hours: Speed, decay rate and morphology have all changed.
 
Oxidation of the phenols is more obvious (black ring), speed has improved, the peg is still producing growth.
Morphology of the mycelium on the agar is no longer cottony, but instead it's flat and linear.
 
Damage from transfer is almost gone, peg growth appears more pushed out.
 
IMG_20181112_192529.jpg IMG_20181112_193112.jpg

----
 
Just noticed a green-blue-black tint, in various areas on the peg, just about visible in the images.
I guess increased nitrogen, and-or increased nitrogen, phosphorus and oxygen?
 
----
 
Baeocystin:  [C11]--[H15]--[N2]--[O4]--[P]
Norbaeocystin: [C10]--[H13]--[N2]--[O4]--[P]
 
Psilocybin:  [C12]--[H17]--[N2]--[O4]--[P]
Psilocin:  [C12]--[H16]--[N2]--[O]
 
 
From above:
 
"Psilocybin is a tryptamine compound with a chemical structure containing an indole ring linked to an ethylamine substituent. It is chemically related to the amino acid tryptophan, and is structurally similar to the neurotransmitter serotonin."
"Psilocybin is a member of the general class of tryptophan-based compounds that originally functioned as antioxidants in earlier life forms before assuming more complex functions in multicellular organisms."
 
"Psilocin is relatively unstable in solution due to its phenolic hydroxy (-OH) group. In the presence of oxygen it readily forms bluish and dark black degradation products"
"Most species of psilocybin-containing mushrooms bruise blue when handled or damaged due to the oxidization of phenolic compounds"
"Psilocin is broken down by the enzyme monoamine oxidase. Some psilocin is not broken down, and forms a glucuronide"
 
 
Notes:
 
Nor-baeocystin are analogs of psilocybin, meaning single or various elements are added or removed.
It appears they can be phosphorylated or dephosphorylated (cleaved, added, a cycle?).
All four compounds contain carbon, hydrogen, nitrogen and oxygen.
 
 
Serotonin:  [C10]--[H12]--[N2]--[O]

Edited by Ferather, 12 November 2018 - 07:20 PM.


#24 Ferather

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Posted 13 November 2018 - 06:12 PM

Today's image, with a modified image to better see the oxidation.
Growth is generally flat, linear, and also populated.
 
IMG_20181113_222310.jpg IMG_20181113_222310_E.jpg


#25 Ferather

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Posted 14 November 2018 - 05:58 PM

Today's image, I conclude Cubensis has the required enzymes to decay cellulose and phenols.
I therefore would take note of the various nutrients and ratios in dung, and it's pH.
 
This makes Cubensis a primary decomposer, but niche (N%, pH or both).
Cubensis also produces various types of protease enzymes.
 
IMG_20181114_221037.jpg
 
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I suggest using 0.5-2g of normal additive with T-Gel, such as malt extract.
 
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T-Gel (plain) and pH modification (not this test):
 
Increasing the pH to ~7, caused 3 types of mycelium to germinate.
The higher pH likely causes the phenols to break down.
 
I got pin mold, trich (green), and a random.
 
----
 
[More images soon]

Edited by Ferather, 14 November 2018 - 08:00 PM.


#26 Ferather

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Posted 15 November 2018 - 07:24 AM

15/11/18 | Morning
 
Visible adaptation, growing towards oxygen, high population rate.
Not the fastest speed, however growth looks robust.
 
IMG_20181115_115217.jpg IMG_20181115_115351.jpg


#27 Ferather

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Posted Yesterday, 11:03 AM

Working as intended, not stalling. My camera, torch and lid are making image quality lower (sorry).
 
Growth is generally uniform, no visible rhizomorphs or overly cottony growth.
Best described as flat(ish), linear and generally populated.
 
IMG_20181117_153424.jpg IMG_20181117_153602.jpg
 
Might need a bit more MG.





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