Cubensis [Data Log]
Posted 29 January 2019 - 08:44 AM
Posted 31 January 2019 - 09:43 AM
Posted 31 January 2019 - 06:46 PM
Nice work Ferather! You probably saw the post I made, but I translated a heap of Jochen Gartz' old German patents. And there is one where he fruits straight off the rice and gets very respectable potency and yield. 100 grams of rice grains produced over 20 grams of mushrooms with from 0.8% to 1.15% total actives. It may be of some interest to you?
A primary culture of Psilocybe cubensis (Earle)Singer was used for inoculation which was obtained by germination of the spores on 100ml of 6% malt agar, 14 days after innoculation and subsequent cultivation (20 days) on 100ml of the same agar. A piece of mycelium about 2 x 2cm was removed from it with a sterile spatula and by shaking with 30ml of sterile water and 15g saddle packing, generated a suspension of fine mycelium flakes. 10ml of the suspension was used to inoculate an autoclaved mixture of 100g rice grains and 200ml of water. After 3 weeks the formation of the fruiting bodies began, which in appeared in 5 fructification waves(flushes).
Yield: 21g dry fruiting bodies
HPLC analysis: Psilocybin content: 0.7%
Psilocin content: 0.1%
Workup is carried out in a known manner by extraction of the powdered material with methanol and subsequent column chromatography on cellulose.
A second strain of Psilocybe cubensis (Earle)Singer was used. Under analogous conditions, the first fruiting bodies were reached after 4 weeks.
Yield: 25g dry fruiting bodies
HPLC: Psilocybin-content: 0.95%
DD254395(A1) -1988- VERFAHREN ZUR GEWINNUNG VON TRYPTAMINDERIVATEN DURCH KULTIVIERUNG HOEHERER PILZE
If you would like a look at the whole patent(its only a couple of pages), let me know.
- Ferather likes this
Posted 01 February 2019 - 07:40 AM
Indeed, thank you. I managed to get 16g from 10g of brown rice and 10g of enriched paper. No idea on potency it was just a test.
I tried to copy outdoor oxygen levels, but had issues with humidity. The fruit bodies where very solid.
Posted 05 February 2019 - 10:29 AM
Edited by Ferather, 05 February 2019 - 10:29 AM.
Posted 08 February 2019 - 06:06 PM
- PistolPete13 likes this
Posted 12 February 2019 - 02:52 PM
Edited by Ferather, 12 February 2019 - 03:24 PM.