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Cubensis [Data Log]


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#61 Ferather

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Posted 29 January 2019 - 08:44 AM

The T-Gel wedge has bleached to almost clear, growth is linear, populated and thickening up, not tomentose or rhizomorphic.
This is the first time this culture has decayed starch (grain), the culture was germinated on WL-Tek + sucrose.
 
Order: WL-Tek + sucrose peg > T-Gel (experimental) > T-Gel (current recipe) > Brown rice.
 
IMG_20190129_125639.jpg IMG_20190129_125745.jpg IMG_20190129_130114.jpg
 
No contamination, odd growth or stalling.


#62 Ferather

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Posted 31 January 2019 - 09:43 AM

Working as intended, growing decently on grain with only 50% total water content (reduces after cook contamination chances).
The grain will re-hydrate when it's spawned to the end media, which will have around 72% water content.
 
IMG_20190131_123819.jpg IMG_20190131_124216.jpg IMG_20190131_124602.jpg IMG_20190131_124702.jpg


#63 PistolPete13

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Posted 31 January 2019 - 06:46 PM

Nice work Ferather! You probably saw the post I made, but I translated a heap of Jochen Gartz' old German patents. And there is one where he fruits straight off the rice and gets very respectable potency and yield. 100 grams of rice grains produced over 20 grams of mushrooms with from 0.8% to 1.15% total actives. It may be of some interest to you?

 

 

 

Example 1

 

A primary culture of Psilocybe cubensis (Earle)Singer was used for inoculation which was obtained by germination of the spores on 100ml of 6% malt agar, 14 days after innoculation and subsequent cultivation (20 days) on 100ml of the same agar. A piece of mycelium about 2 x 2cm was removed from it with a sterile spatula and by shaking with 30ml of sterile water and 15g saddle packing, generated a suspension of fine mycelium flakes. 10ml of the suspension was used to inoculate an autoclaved mixture of 100g rice grains and 200ml of water. After 3 weeks the formation of the fruiting bodies began, which in appeared in 5 fructification waves(flushes).

 

Yield: 21g dry fruiting bodies

HPLC analysis: Psilocybin content: 0.7%

Psilocin content: 0.1%

 

Workup is carried out in a known manner by extraction of the powdered material with methanol and subsequent column chromatography on cellulose.

 

Example 2

 

A second strain of Psilocybe cubensis (Earle)Singer was used. Under analogous conditions, the first fruiting bodies were reached after 4 weeks.

 

Yield: 25g dry fruiting bodies

HPLC: Psilocybin-content: 0.95%

Psilocin-content: 0.2%

 

 

source;

DD254395(A1) -1988- VERFAHREN ZUR GEWINNUNG VON TRYPTAMINDERIVATEN DURCH KULTIVIERUNG HOEHERER PILZE

 

If you would like a look at the whole patent(its only a couple of pages), let me know.


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#64 Ferather

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Posted 01 February 2019 - 07:40 AM

Indeed, thank you. I managed to get 16g from 10g of brown rice and 10g of enriched paper. No idea on potency it was just a test.

I tried to copy outdoor oxygen levels, but had issues with humidity. The fruit bodies where very solid.

 

Cubensis-Fruits-1.jpg Cubensis-Fruits-2.jpg Cubensis-Fruits-3.jpg Cubensis-Fruits-4.jpg



#65 Ferather

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Posted 05 February 2019 - 10:29 AM

Getting there, 1 week 2 days now. Roughly the same speed as ME agar, but for a different reason.
ME agar is high in water (something like 95%), whereas my grain is 45-50% water.
 
Bottom growth is thicker and better looking, it also has more water.
 
IMG_20190205_133013.jpg IMG_20190205_133120.jpg
 
====
 
How I assembled the brown rice:
 
24 hour soak in cold water, strained, and then microwaved dry (900W). The idea, to germinate endospores, then: heat, dehydrate and crush them - very rapidly.
I then microwave PC'd the rice for 18 minutes with capacity + cook water: Add water, add a bowl, add container with rice and water.
 
After cooling the rice had a bit of a smell from partial anaerobic hydrolysation, I reheated my rice, with the lid off.
The smell changed to a cooked rice smell, as a positive effect, all excess water was removed.
 
The PC was set for rice cooking which was vent closed (maximum pressure).
 

Edited by Ferather, 05 February 2019 - 10:29 AM.


#66 Ferather

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Posted 08 February 2019 - 06:06 PM

I will be spawning in a few days, I have vigorous growth.
There's some rice in there somewhere...
 
IMG_20190208_202026.jpg IMG_20190208_202038.jpg

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#67 Ferather

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Posted 12 February 2019 - 02:52 PM

 
----
 
As soon as I opened the container (sudden increase in oxygen) some aerial mycelium bruised, about 4 seconds after mixing several areas bruised.
Smell, 50-50 fungi-honey (glucose in various formats, from starch), I apparently have hyper glucose production (more than normal).
 
This most likely is due to T-Gel, where phenols (not made from glucose) must be converted into glucose.
I have spawned two containers, both are the same amount, both are WL-Tek as the media.
 
 
IMG_20190212_183504.jpg IMG_20190212_183509.jpg IMG_20190212_192341.jpg IMG_20190212_192521.jpg

Edited by Ferather, 12 February 2019 - 03:24 PM.





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