Oh yeah, not too soon. That's why we tag it--so we can search "archive material" later after the dust has settled on the thread.
And I will do agar, probably by this summer.
Posted 25 February 2018 - 02:15 PM
Oh yeah, not too soon. That's why we tag it--so we can search "archive material" later after the dust has settled on the thread.
And I will do agar, probably by this summer.
Posted 02 March 2018 - 02:10 PM
I really need to get some jars like those. I've had a little anxiety thinking about ordering plates all the time... So wasteful :D So we dont need filters or anything added? Good to know :D
Posted 02 March 2018 - 02:28 PM
Edited by peacefrog, 02 March 2018 - 02:30 PM.
Posted 04 March 2018 - 12:56 AM
Alright, I need y'all to imagine some things in this video/gif. I've been meaning to "film" how to grab myc and slice it into new agar. In this footage I need y'all to imagine that I'm wearing gloves like we should always do. I'm not wearing them here because I can't hit the record button and put my gloves on and make this vid/gif short. I also need y'all to imagine that I'm slicing that wisp of myc into a new (blank) agar plate.
So I took the blunt side (but still the tip) of my scalpel, dragged it through the desired myc (I had already grabbed the myc I wanted before recording this, so here I grabbed lower for educational purposes
) and then sliced it into the agar as if it were a new plate.
Understand rubber band?!
I posted this here to show newer agar enthusiasts how I grab myc with a scalpel.
It should be noted that one should grab a much smaller piece if possible, but here I want the viewer to see the wisp so I dragged the scalpel more than necessary.
Edited by CatsAndBats, 04 March 2018 - 01:18 AM.
Posted 04 March 2018 - 01:30 AM
Posted 04 March 2018 - 01:47 AM
Nicely done, brother. I need you to teach me about these gifs lol.
Thank you my main man peace!
I just record with my phone's regular video and then use an android app to convert video to gif (free in the play store), upload it and here we are. The only real challenge is to make it short/small enough to be a reasonable size. I don't want to kill mycotopia's server space/bandwidth, but I also don't want to leave a youtube "footprint", so I convert it to a gif.
Posted 04 March 2018 - 01:53 AM
"just the tip"
Posted 04 March 2018 - 01:57 AM
Edited by peacefrog, 04 March 2018 - 02:09 AM.
Posted 04 March 2018 - 02:26 AM
Do you ever find that not taking more agar with you during the transfer can allow the transferred peice to dry out before it takes hold? I only ask cause I have had that happen in the past.
No I do not. I suspect that I've never noticed that happening because I do 3-5 transfers per desired result. So if I throw out four plates per five tries then I'm still "successful" (that was hyperbole, I usually only throw out a couple jars per twenty or so). I employ a numbers friendly technique.
When I do an agar night, I blast through 15-20 jars, so if one or two don't take, I barely notice, those jars go to the compost pile without a second thought.
Posted 04 March 2018 - 02:43 AM
Posted 04 March 2018 - 10:32 AM
Law of averages works every time IME. Nice dude.
The extras just become inoculant. :biggrin
Posted 04 March 2018 - 04:16 PM
wait do you actually throw out these "plate" jars or can we generally reuse them - minus like trich and stuff ?
Also, thanks for the gif :D
Edited by ipc, 04 March 2018 - 04:16 PM.
Posted 04 March 2018 - 04:58 PM
wait do you actually throw out these "plate" jars or can we generally reuse them - minus like trich and stuff ?
Also, thanks for the gif :D
Noooooooo, I just throw out the agar inside the jars and wash them. As soon as I have 15 empty, I do another batch.
Oh and you're welcome!
Posted 05 March 2018 - 05:58 AM
Posted 05 March 2018 - 07:34 AM
Hey Cat, do you know if your agar produces better results than plain old dog (or cat) food agar?
Posted 05 March 2018 - 12:10 PM
Hey Cat, do you know if your agar produces better results than plain old dog (or cat) food agar?
Let's be clear, agar is an open ended tool that one can use however one sees fit, adjusting to one's style/goals. My style is to do large runs, let's call it "agar carpet bombing". I play a numbers game and use all my extras as inoculant for quart jars, as I'm isolating out desired genetics and a true mono culture/s at the same time. I switch up my nutrition to keep the myc interested and because I like fucking around with recipes.
As seen here:
https://mycotopia.ne...r-30/?p=1358542
And gave me the experience to answer this with confidence:
https://mycotopia.ne...r-30/?p=1358111
Dog food agar was my first successful agar recipe and I never looked back. I am of the opinion that every agar enthusiast should have 2-3 go to agar recipes. None of mine are better, but they serve a purpose (usually )!
Posted 06 March 2018 - 07:23 PM
For those of you following along, that tallest strand is where I'd grab myc for the next transfer. It's also easier to free the myc from glass than from agar. Cheers!
Posted 07 March 2018 - 03:34 PM
Posted 07 March 2018 - 04:12 PM
Posted 07 March 2018 - 04:36 PM
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