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Cubensis Mono-tubbing Adventures with peacefrog


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#1 peacefrog

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Posted 07 May 2018 - 06:50 AM

I haven’t done a good old bulk cube grow in a few years, but decided to do one for the fun of it. And I went back to my favorite substrate for this grow for nostalgic reasons, cased plain pasteurized wheat straw.

It seems that I do not see many newer members using straw alone for bulk these days for actives. I used to use it exclusively for cubes back when and loved it. If prepared and treated properly, it makes for a very awesome substrate for many higher fungi. It of course takes a bit more work and attention to detail verses coir, but still one of my favorite subs to work with.

Let’s get started. I decided to do a MS grow of P. cubensis PES Amazonian. I am working on other projects at this time, and I wanted to grow this fast as possible, so no isolation past clean growth was done here.

Master grain jar fully colonized:
F593CEA5-CD66-4C73-83B2-E6F9759BCA6C.jpeg

G2G spawn jars fully colonized:
03EFAF8B-C4D7-4B55-AF3F-42782EDC044A.jpeg

Wheat straw cut up and ready for pasteurization using the good old pillow case tek:
E0E6E6C2-C9D3-4F8C-B909-1D7484D6FE16.jpeg


If using the pillow case tek, there is one trick that I highly recommend to anyone. Place the pillow case/or other porous vessel in the pot and add water to it until the water level is 2-3 inches or so above the pillow case. You will have to physically hold it down with your hands as it will tend to float. Then once the water level is where you want it, remove the case from the water and let drain for a few minutes back into the pot:
BADD4643-9615-45B5-BF9C-07EF13C99C43.jpeg 95AE3AF0-E6DE-4A37-B336-E8461E2DF8F8.jpeg E3BE2BEC-75EE-4026-BEC0-61C660D97112.jpeg C43DA463-4573-4747-9181-BEE9E5E42DB2.jpeg 3630C086-E716-4695-96E8-66D7A15BE569.jpeg 3454D8B9-1961-4D24-BACA-546EA3EAF2EB.jpeg

Pasteurizing temps are generally 140-160 degrees Fahrenheit. So now, bring the pot of water up to 140 degrees. Carefully place the vessel into the water and weigh it down (I use 5-pound dumbbells) and monitor the temperature of the water. If the temps drop below 140, just stop your time and bring it back up to the desired temps before starting the time again. If the temps go above 160, don’t worry too much, just get the temp down quickly as possible. Hold these temperatures for a minimum of 1 hour (2-3 is better IME):
8F364B87-C814-4DFC-9068-1D52FFC3A02A.jpeg

After the time is up, remove very carefully, let it drain as much as possible in the pot, and place it somewhere clean to cool and drain the remainder of the water out. I use a large clean tote to do this. I place the pillow case on top of a colander and allow to drain and cool fully:
F31D0D26-644F-4F31-9861-CC9DB4515038.jpeg

Spawned and tightly compacted straw:
50A7A4AA-9CB2-49BC-A127-8548D5E52C1F.jpeg

3 days later and the mycelium is starting to run:
A31A9F84-54CF-46FD-80F6-7B21DA7B360E.jpeg 0E2BF199-9D52-42AD-8E8F-2D4E4DD98674.jpeg 6AE40DD5-080E-49E1-8F4A-8C0F434D4051.jpeg

Fully colonized straw 9 days later (12 days after spawning):
74F26FF3-6C73-4C17-97DA-3FF3C245A221.jpeg 7A089343-829B-40C0-9C0B-B2458740B634.jpeg

Casing added (per my standard pasteurized 50/50+). Since straw tends to dry out very quickly, I always used to case it thick with cubes. I have placed a 1-1.5” layer of casing material on top to help keep my sub from drying out too quickly and to aid in keeping my microclimate at the maximum. It takes longer for the casing to colonize with this thicker casing layer, but will aid in keeping the straw from drying out over multiple flushes:
25DAABE1-A63E-40A1-A6C1-5B17A23140D9.jpeg BEF26410-2638-4FE2-A09D-B2B4ED0BB8A0.jpeg

Casing run complete and now set into fruiting mode (i.e. introduction of FAE):
B5C34132-E356-4AE3-BB04-74B32BCFC247.jpeg DAEAD514-E03B-4140-BB19-E81C8583151A.jpeg

I am old school in this practice of letting the casing run occur with GE only and introducing FAE once the casing run is complete. There are many growers that introduce FAE after spawning and/or after casing. There are plenty of examples on the OMC where that practice performs well. I am just stuck in my ways on certain things. What’s the saying, “if it isn’t broken don’t fix it” lol. I only say this cause if you have done this hobby for a while, you know there are many ways to successfully cultivate mushrooms. The only “best way” is the one that works for you. This is just my personal preference with cubes.

1st flush:
EEB7E5AD-5236-40B8-A86B-C04C63419908.jpeg 29BBA4F7-0A56-4CED-AC5F-63CF939B5734.jpeg

2nd flush and the boomers came (very nice large specimens):
2777163B-8F3B-43CD-AD02-4C99F015E311.jpeg 6E7C1887-FBEC-4CC2-8EC0-9E1CEC3E4AA9.jpeg 1B3F6723-112C-42D2-B635-F692C0A268BE.jpeg 167F598A-62D7-42E8-978E-E20B5123DE1C.jpeg

I threw the fruits and the substrate out after the 2nd flush. I am just not a cube guy anymore and am not going to keep them, but I had fun working with them and utilizing my old favorite substrate, straw. For 6-10 bucks you can purchase a bail that will last a LONG time! As stated above, it just takes more time and more attention to detail verses coir. But is one of the best and versatile subs I have ever used for cubes, if treated and spawned correctly.

A side note, check the gnarly huge invitro specimens at the bottom after harvesting the 2nd flush:
01417A0A-5B2E-4A1A-94C1-B8B37843840E.jpeg

And as always, happy growing and good vibes!

 

Acronym guide https://mycotopia.ne...ms/?hl=acronyms


Edited by wharfrat, 12 May 2018 - 02:06 PM.

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#2 Microbe

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Posted 07 May 2018 - 10:22 AM

That is the most economically sound substrate IMO other then dirt. Well i guess some people have access to free stuff.

Nice write up and i believe you demonstrated the most critical factor for runing 100% straw......Caaaaaaasing. I learned the hard way and it dried out constantly!
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#3 Hash_Man

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Posted 07 May 2018 - 12:06 PM

Nice Peacefrog.. straw brings my back ...
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#4 ipc

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Posted 07 May 2018 - 01:40 PM

those are some monsters!good stuff dude

 

how often did you fan/mist with this holeless tub. i think i dont see any anyways


Edited by ipc, 07 May 2018 - 01:42 PM.

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#5 wharfrat

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Posted 07 May 2018 - 01:57 PM

beautiful  :thumbs_up: 


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#6 bottlebomber

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Posted 07 May 2018 - 05:35 PM

Awesome write-up, you make it look easy. Thanks for taking the time to do that, very comprehensive. It’s just breaking my heart to think of the cubes going to waste
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#7 PsyBearknot

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Posted 08 May 2018 - 06:03 AM

This will be one for the archives
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#8 Bonsai911

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Posted 10 May 2018 - 08:46 PM

I have got to give that a try!
Thanks for the inspiration Brother.
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#9 Sidestreet

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Posted 11 May 2018 - 04:27 AM

Great writeup and grow, thanks!  It's definitely archive material and I'm adding it to your member library.


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#10 Arathu

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Posted 11 May 2018 - 09:08 PM

Oh my just awesome dude! Ah the cube days................very nice work frog......

 

A


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#11 bezevo

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Posted 12 May 2018 - 03:42 AM

very interesting 

could  you  say what your standard 50/50 casing is

thanks


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#12 peacefrog

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Posted 12 May 2018 - 04:09 AM

those are some monsters!good stuff dude
 
how often did you fan/mist with this holeless tub. i think i dont see any anyways

I never fan really with monos. I leave the lid loose with a gap on 1-2 sides when I’m away. When I’m home, I leave the lid completely off for a few hours, and mist when needed.

Awesome write-up, you make it look easy. Thanks for taking the time to do that, very comprehensive. It’s just breaking my heart to think of the cubes going to waste

Yea I know. I just will never ingest them, so why keep um is my thoughts. Back when, I would thought it blasphemous to toss them out like that lol. But I just did that grow for the fun of it, as I haven’t done one like that in a few years now.

very interesting 
could  you  say what your standard 50/50 casing is
thanks

A detailed recipe can be found here: https://mycotopia.ne...pans-grow-log/.

But I still recommend testing a few batches for ph levels, just In case. But this recipe has worked well for me using many different brands peat.

Thank you for the kind words guys!
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#13 bezevo

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Posted 12 May 2018 - 04:51 AM

thanks very interesting and informative



#14 Sidestreet

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Posted 12 May 2018 - 07:27 AM

 

I never fan really with monos. I leave the lid loose with a gap on 1-2 sides when I’m away. When I’m home, I leave the lid completely off for a few hours, and mist when needed.

 

Interesting, I wouldn't think that would be enough air movement but obviously it is!


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#15 peacefrog

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Posted 12 May 2018 - 07:35 AM

Yes sir I have ran them this way for several years now and I always do the same with copes. Seems to produce plenty of fresh air for um.

Edited by peacefrog, 12 May 2018 - 07:36 AM.

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