515 replies to this topic

[PinkMenace]

Let's grow some sclerotia producing mushrooms! I have a Psilocybe mexicana var tampanensis print I'm cutting up and sending to @psybearknot and wanted to know who else wants to join us in this thread (you're on your own for spores, though). The plan is to fruit them for the mushrooms, but I'm also going to put aside some jars for a rainy day. 
 
Here are some links to get acquainted:
 
A Comprehensive Sclerotia Cultivation Guide-From Spore Print To Spore Prints(VER 1.0)  - sunstone
 
Psilocybe Tampanensis Multispore Mycobag - CaptainFuture (it should be noted tampanensis is now considered as being of the species mexicana)
 
Psilocybe tampanensis - waylit (covers some agar information)
 
Exotic Magic Mushroom Species:MEXICANA, SCLEROTIA (vault)
 
The Shadowbox - A Low Maintenance Universal Fruiting Chamber Tek + Easy Ps. Mexicana Grow Tutorial

Edited by coorsmikey, 23 September 2018 - 02:51 PM.

[Kraven]

I dont have access to those spores right now, so I'll watch....I started AA+, Red Boy, PE, Burma plus expanding a cube[Florida wild P. cubensis] thats a clone so this will be the first clone run of this cube..I also have 6 trays of P. cyan Cambodian "Goliath" I just started fruiting yesterday....My plan was to kncok up a few jars and let them sit while I work this round..plus if the got knocked up now it would be between Christmas and Thanksgiving coming off, so not real great timing. Have a blast and

[PsyBearknot]

Hey Pink.
Thank you for sharing...fo SHO!

This one has come and gone from my hands a couple of times.

I have colonized and incubated many quart stone jars in the past with lack luster results. Lots of big looking stones up against the glass that turn out to be thin and flat when harvested to large pea size you just wonder is the corn you used and not true stone.
Then there is the metallic bitter taste

Around the same time a couple years back Wharfrat and I had a couple of tray grows about the same time. I was making an attempt to fruit at that time. I got no fruits but got 2 stones about the total size of a
Golf ball. First successful trip from them and agree with the general reports as the trip being similar but different and “more friendly” but can still kick you in the ass

My interest in the fruit also is in the small but plenty mushrooms thst grow I. Slightly smaller /
Thinner substrates like the pans seem to like

I plan on starting from agar and have a couple of ideas I’d like to try. I have seen starting by agar that produces a stone on the agar and cloning from there.

I know hash man has also done some growing as well. Hopefully he and others will join in when able

[Oneyedraven]

Pink - I think I might be interested.. I'm a true neophyte though probably as with most of you the successful grow with the PF Tek , MFTM is doing awesome.. still trying to find the fanning sweet spot tho lol.  Getting supplies together to start LCs  and clones on agar.  Took a peek at the Tek links you included , can this be done in LC or agar cloned? If so I'm in 

might need extra coaching on the grow. let me know .. awesome 

[PinkMenace]

Pink - I think I might be interested.. I'm a true neophyte though probably as with most of you the successful grow with the PF Tek , MFTM is doing awesome.. still trying to find the fanning sweet spot tho lol.  Getting supplies together to start LCs  and clones on agar.  Took a peek at the Tek links you included , can this be done in LC or agar cloned? If so I'm in 

might need extra coaching on the grow. let me know .. awesome 

 

You will need to get some spores of sclerotia (stone) producing mushroom to follow along. Once you have the spores, it's up to you how you want to germinate those spores and then spread the mycelium around. You could for example grow your spores out on agar, then transfer that agar to a small grain jar for milking or to an LC. Cloning usually comes into play once you already have a fruit body or sclerotia, though it's possible to grow sclerotia on agar and then clone that for maximum results.

 

If you're super new, I'd do a couple cubensis grows on cakes then bulk first unless you're not discouraged by failures. And we will absolutely help you, regardless. :-).

Edited by PinkMenace, 20 July 2018 - 05:22 PM.

[Oneyedraven]

I am super new.

What... I don't get to skip a grade? lol . Think I'll take the advice and do a few more cakes then bulk. I don't take failures very well. Probably good to have the basics down and confidence up first before I sit at the big kids table.Thanks for the advice

[PinkMenace]

I am super new.

What... I don't get to skip a grade? lol . Think I'll take the advice and do a few more cakes then bulk. I don't take failures very well. Probably good to have the basics down and confidence up first before I sit at the big kids table.Thanks for the advice

 

Always have a couple things in the pipeline to make the failures sting a little less. When you want to do a stone grow, let me know and I'll do another :-).

[PsyBearknot]

Here is the Grow I did with the mega stone
Last page of the thread has most of the info

https://mycotopia.ne...box-subs/page-4

Edited by PsyBearknot, 20 July 2018 - 11:07 PM.

[Soliver]

I'm down with the stone.  It's been too long . . .

 

Anyone / someone PM me with your (reputable) spore supplier, and it's on like donkey kong!

 

 

:)

 

soliver

[PsyBearknot]

I am super new.
What... I don't get to skip a grade? lol . Think I'll take the advice and do a few more cakes then bulk. I don't take failures very well. Probably good to have the basics down and confidence up first before I sit at the big kids table.Thanks for the advice

The making of spawn jars used for the most basic tek for growing the stones is usually a lot of times the next step for some or even an inbetween step. This process is truly the ultimate set and forget / neglect tek
Quart jar with hydrated grain is inoculated by spore syringe and then put in a closet
For 6-9 months
Many start this type project while waiting for their brf jars or bulk substrate to colonize or fruit
So get the spores when you can and jump in any time...
...your really just a stones throw away ...

Edited by PsyBearknot, 20 July 2018 - 11:20 PM.

[PsyBearknot]

I'm down with the stone.  It's been too long . . .
 
Anyone / someone PM me with your (reputable) spore supplier, and it's on like donkey kong!
 
 
:)
 
soliver

Whudup stoner!?!

[Da-1]

 

I am super new.
What... I don't get to skip a grade? lol . Think I'll take the advice and do a few more cakes then bulk. I don't take failures very well. Probably good to have the basics down and confidence up first before I sit at the big kids table.Thanks for the advice

The making of spawn jars used for the most basic tek for growing the stones is usually a lot of times the next step for some or even an inbetween step. This process is truly the ultimate set and forget / neglect tek
Quart jar with hydrated grain is inoculated by spore syringe and then put in a closet
For 6-9 months
Many start this type project while waiting for their brf jars or bulk substrate to colonize or fruit
So get the spores when you can and jump in any time...
...your really just a stones throw away ...

 

 

I can`t wait to join you guy`s and gal`s at throwing stones at glass houses 

 

 

I'm down with the stone.  It's been too long . . .
 
Anyone / someone PM me with your (reputable) spore supplier, and it's on like donkey kong!
 
 
:)
 
soliver

Whudup stoner!?!

 

 

whaaaat uuuuup!!!!!!!!!!!!!!!!!!!!!!! 

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[bezevo]

SUBSCRIBE

[PinkMenace]

Mailed the spores! I'll wait until bear gets them to get going. Soliver, did you get hooked up with info?

[PsyBearknot]

<looks in mail box>

DAMN NOT THERE


<looks in mail box>

DAMNIT

<looks in mail box>

Arrrgggg

<looks in males box>

DOH! Wrong slot....I’ll be back later...

[RamenShaman]

I will be following this thread good vibes to all involved in this endeavor

[Soliver]

Who's got the good P. Mex - anyone?

Mailed the spores! I'll wait until bear gets them to get going. Soliver, did you get hooked up with info?

:) soliver

Edited by PsyBearknot, 24 July 2018 - 08:55 PM.

[PinkMenace]

I just read two contradictory things regarding caffeine/coffee and sclerotia development. 

 

The first is from Hippie3 who says here in 2004 that he "read that caffeine has been correlated with a boost in sclerotia formation with mexicana". This would explain the coffee soaks in some mexicana teks.

 

However, in a response to Mycotopia post Caffeine, Tannins, and Polyphenols from Coffee Residues Bad for Mushroom Growing user Invitro cites a scientific article entitled Increase in Endogenous and Exogenous Cyclic AMP Levels Inhibits Sclerotial Development in Sclerotinia sclerotiorum (pdf) saying "Caffeine, IBMX, and NaF all block sclerotial development and are expected to increase cAMP levels. Elevated cAMP levels appear to block early sclerotial development since sclerotial initials are not observed or are greatly reduced in number in cultures amended with cAMP. Developing cultures transferred to cAMP-containing medium before sclerotial initiation did not produce sclerotia, whereas cultures transferred after sclerotial initiation continued to develop mature sclerotia. Although cAMP regulates sclerotial development, the nature of this regulation remains uncertain. cAMP regulation may be indirect in that signaling through a cAMP-dependent pathway may stimulate filamentous growth, resulting in hyphae which fail to differentiate into sclerotia." The species of mushroom here is S. sclerotiorum, but I imagine the knowledge is transferable.

 

I hate to say it, but citation wins over no-citation. I will not be mixing in a caffeine source.

Edited by PinkMenace, 24 July 2018 - 09:14 PM.

[PsyBearknot]

That could be why a lot of people who use coffee in a soak make an”weak” batch for the soak and use spent coffee grounds in their substrates but then again most of that is not for a sclorita producer grow.

[PinkMenace]

That could be why a lot of people who use coffee in a soak make an”weak” batch for the soak and use spent coffee grounds in their substrates but then again most of that is not for a sclorita producer grow.

 

The weak batch is because of the acidity, I believe.