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Stone producer group grow


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#21 PinkMenace

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Posted 24 July 2018 - 10:13 PM

@425nm noted that these fungi (Sclerotinia sclerotiorum) may function differently than the mushrooms we are interested in (Psilocybe mexicana and friends). They are a fungus of a different phylum (Ascomycete) and are at least in someways a very different beast.

 

Anyone know if what's transferable in that paper between species? @Microbe?


Edited by PinkMenace, 24 July 2018 - 10:15 PM.


#22 Microbe

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Posted 25 July 2018 - 09:44 AM

@425nm noted that these fungi (Sclerotinia sclerotiorum) may function differently than the mushrooms we are interested in (Psilocybe mexicana and friends). They are a fungus of a different phylum (Ascomycete) and are at least in someways a very different beast.

Anyone know if what's transferable in that paper between species? @Microbe?

I believe it is transferable. Here is one of my favorite resources to go to for stuff like this. Its not specific to sclerotia and does touch in it a bit, I feel that caffeine is detrimental to many species of fungi including sclerotia producing species. It can even be used as a biocontrol agent against other organisms including trichoderma.

https://www.ncbi.nlm...les/PMC4871636/

Edited by Microbe, 25 July 2018 - 09:46 AM.

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#23 Soliver

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Posted 25 July 2018 - 10:48 AM

OK kids - I'm in!  Hopefully have the spores in a week(ish) . . .

 

Who's got the daddy spawn recipe?

 

:)

 

soliver



#24 bezevo

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Posted 25 July 2018 - 01:05 PM

On the chat last night there was a bit of discussion of the possibility that  stone production might be triggered when nutrients were running out .   

There were suggestions of using grains with  less nutrients and smaller jars .so based on this discussion , i am guessing ........that possibly using popcorn ... less nutrients ....and .....small jars.... like pints instead of quarts might  be worth trying ?

 

Any thoughts or  Speculation  internet experts ?


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#25 RamenShaman

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Posted 25 July 2018 - 01:12 PM

I have seen many stone grows on popcorn. Pints may produce stones faster but quarts will likely produce more in volume.

Also for those interested in fruiting these guys I have read that the fruits originate from the sclerocia so harvesting all of the stones before spawning May hinder the fruiting phase.

I have no sources to back this up but may be something to look into.
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#26 Soliver

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Posted 26 July 2018 - 06:56 PM

My first and last stone grow was on popcorn . . .

 

One major benefit of using popcorn is that you don't have to pick annoying tidbits of tiny grains from the stones.  At first, I wasn't sure what was corn and what was stone, but once they dried for a day, the corn kernels separated from the stones, and - voila! 

 

FYI - the popcorn that grew around the stones were also highly active.  For all I know, the whole goddamn jar may be - again, I didn't do much research, but I'm ready to do so   :)

 

xxoo

 

:)

 

soliver


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#27 bezevo

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Posted 26 July 2018 - 10:04 PM

hummm  active corn nuggets ....  interesting



#28 Microbe

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Posted 27 July 2018 - 06:54 AM

On the chat last night there was a bit of discussion of the possibility that stone production might be triggered when nutrients were running out .
There were suggestions of using grains with less nutrients and smaller jars .so based on this discussion , i am guessing ........that possibly using popcorn ... less nutrients ....and .....small jars.... like pints instead of quarts might be worth trying ?

Any thoughts or Speculation internet experts ?

I would think there is some validity to this. I do know that stones are produced by certain species as a survival mechanism. Its purpose is similar to how other species create what's called chlamydospores which is a thick cell walled hyphae that functions as a spore and is intended to survive harsh conditions.

I have yet to attempt to produce any stones yet but when I do, im certainly going to do it in less then ideal conditions including but not limited to, low nutritional substrate, extreme temps, minimal gas exchange, and potentially introducing a competitor.

#29 PsyBearknot

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Posted 27 July 2018 - 07:33 AM

Where this chat conversation came from was an idea I had for germinating spores on a standard nutrient level agar and then stepping down with each transfer in nutrients to see if I could force a stone to grow on the agar quicker or easier and then clone that stone to then take back to a grain jar such as white rice or a corn jar with white rice courtly ground as the first food source

Another thought I had thrown around was making a deeper non nutrient agar jar and dropping a piece of hydrated corn so it is submerged completly to one side of the agar. as one of the step down transfers Place the transfer to one side of jar and let the mycelium find the corn to see if you can force a stone to grow. Thought being let the myc spend energy finding the low nutrient corn and then decide to use the space between the agar and the corn to start building a stone. Stones form between corn in corn spawn jars and also against the glass. This would give the space between the corn and the glass and the corn and the agar.

Lastly thinking on the way to work this morning of the grows I have done with the one coir based substrate and the jar attempts. The coir based sub produced a large stone and the corn based jars produced many pebble size of low potancy. This led me to the thought of the weight or compactness of the soil effects the size. Was it hippie3 that did the spawn bags tight full of grain? I think the coir based substrate mimicked the more natural soil structure.

Use something to the grain isolation tek and colonize one layer of corn in a jar. Then give it a layer of brf or other substrate to get the myc running and the a thick casing layer for the stones to grow in and then eventually fruit out the top while the stones are aging. Harvest the fruit bodies and then let the stones age more as the substrate starts to dry out forcing the stones to start bulking up thinking it’s time to preserve its self since there are no longer proper fruiting conditions.

Edited by PsyBearknot, 27 July 2018 - 07:49 AM.

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#30 PinkMenace

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Posted 27 July 2018 - 11:09 AM

[...]
Use something to the grain isolation tek and colonize one layer of corn in a jar. Then give it a layer of brf or other substrate to get the myc running and the a thick casing layer for the stones to grow in and then eventually fruit out the top while the stones are aging. Harvest the fruit bodies and then let the stones age more as the substrate starts to dry out forcing the stones to start bulking up thinking it’s time to preserve its self since there are no longer proper fruiting conditions.

 

Wowzer, loving this. Essentially do an invitro grow of both stones and fruit at the same time! Gee golly!

 

Also, do you mind if I get started with the agar process this weekend or should I wait for you?


Edited by PinkMenace, 27 July 2018 - 11:09 AM.

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#31 PsyBearknot

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Posted 27 July 2018 - 11:31 AM

GO GO GO! ! !

#32 onediadem

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Posted 27 July 2018 - 01:45 PM

Has anyone here ever actually dosed with these? 



#33 PsyBearknot

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Posted 27 July 2018 - 02:16 PM

Yes

#34 Da-1

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Posted 27 July 2018 - 02:27 PM

Hiya Onediadem

 

I have had them once and it was amazing, a lot different then just eating fruit bodies more clear headed and I mainly got visuals towards the end, during the journey I faced a lot of self inner issues and I also thought I was communicating telepathically with the friends I was with, during the trip I also noticed that when closed eyed it felt like my eyes were still open and I could see my surroundings, I know that sounds strange but it is what it is I guess, in the morning after the trip as I battled to sleep even after smoking so many joints, making smoke was really hard as everything looked like worms in my hands but all I wanted was another smoke, I even got my friends girl to make them and she does not even smoke lol, so when I did wake about 3 or 4 hours later I felt rejuvenated as I always do when journeying on this scared friend of wisdom, sorry to sidetrack the topic just needed to pipe in.

 

P.s I can`t wait to join you gents and ladies at throwing stones, I am in the process of trying to locate some old friends in the hopes of getting some print in a trade 

 

Regards, Da-1



#35 onediadem

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Posted 27 July 2018 - 02:31 PM

The reason I ask is that I tossed over a LB last year because I wasn't sure if it was just a novelty or a bad strain. Friends did not get anything from them, and I felt hardly anything and I am very sensitive to our fungal friends. I kept upping the dose thinking ok this will do it, but nope. A few slight visuals, some tingly body load, but all in all I just didn't get it. They were beautiful stones too. So, yes, I am just curious if what I experienced was the norm? I know they are "philosopher" stones, and it has made me think a lot about the whatever aspect lol.


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#36 PsyBearknot

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Posted 27 July 2018 - 03:15 PM

The one I ate that was from the coir based substrate worked. All Other attempts have failed.

#37 Soliver

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Posted 27 July 2018 - 03:53 PM

I dosed P. Mex I grew in corn - I have the writeup somewhere, but I essentially lost space and time while in the shower, with my glasses on the counter - I couldn't make sense of anything by the time I got out of the shower and spent an unquantified period of time crawling around naked and wet looking for my glasses (a towel would have been a good idea too). 

 

I lowered the dose for future experiments.  The funny thing is I literally couldn't give them away, as no one knew WTF they were, and most of them dried out hard as rocks.  This was before I discovered extractions, and I had tons of shrooms on hand anyway.  Don't remember what happened to the rest, but .. yeah, for me they were pretty impressive.  I'm looking forward to trying again  :)

 

soliver


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#38 PsyBearknot

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Posted 27 July 2018 - 04:39 PM

I think that a reason why people have underwhelming experiences is because the stones dries so hard and it takes so long for them to digest that you just don’t get the goodies in your system quick enough before they start to breakdown

#39 onediadem

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Posted 27 July 2018 - 08:39 PM

You aren't kidding, they call them stones for a reason. Even before they dried though, they were pretty hard. I did try breaking one with a hammer and it hurt my hand, so they went into the trash. No way was I going to mess up my grinder for something that did nothing for me. To me, it was just a pretty lackluster grow experience until I was able to harvest them and found them. I had quite a few pretty large ones but most were nickel size. And your right, I couldn't give them away either, and the friends who did try them didn't want any more of them.


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#40 PsyBearknot

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Posted 27 July 2018 - 09:05 PM

It yet they thrive in Amsterdam in the shops. Somebody has the process down and working it gotta be something behind it.




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